Depletion is used to reduce the complexity of biological samples such as serum, plasma, or biofluids, which contain high concentrations of albumin and immunoglobulins. These products utilize immunoaffinity techniques to enable the removal of the most abundant proteins, thus enhancing the detection of lower-abundance proteins in both discovery and targeted proteomic analyses. 

  • Efficient—kits remove > 95% of albumin and > 90% of IgG (Top 2), plus other abundant proteins (Top 12)
  • Fast—spin column formats enable the processing of samples in less than an hour
  • Economical—cost-effective spin columns are priced for single use
  • Consistent—one-time use helps prevent abundant protein carryover and experimental variability

 

Choose the right abundant protein depletion product for your mass spec application

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  Pierce™ Albumin Depletion Kit Pierce™ Top 2 Abundant Protein Depletion 
Spin Columns Pierce™ Top 12 Abundant Protein Depletion 
Spin columns
 
Pierce™ Albumin
Depletion Kit

Pierce™ Top 2 Abundant Protein Depletion Spin Columns

Pierce™ Top 12 Abundant Protein Depletion Spin columns
Proteins depleted Albumin Albumin, IgG Albumin, IgG,
and 10 other proteins*
Sample volume capacity 10–50 µL 10 µL (600 µg) 10 µL (600 µg)
Processing time 20–30 min 40–60 min 40–60 min
Format Loose resin, spin columns, buffers Pre-filled spin columns Pre-filled spin columns

*Top 12 proteins include albumin, IgG, apolipoprotein A-II, fibrinogen, α1-acid glycoprotein, haptoglobin, α1-antitrypsin, IgA, α2-macroglobulin, IgM, apolipoprotein A-I, transferrin


Video for abundant protein depletion

Featured product data

Protein removal achieved using Pierce™ Top 12 Abundant Protein Depletion Spin Columns (Cat. No. 85165). Values were determined by targeted mass spectrometry. The albumin depletion percentage was cross-validated by ELISA and was in agreement with >99% removal.

Protein Fold reduction Percent depletion
albumin 3369 99.97
transferrin 266 99.62
alpha-1-antitrypsin 37 97.30
haptoglobin 127 99.21
alpha-1-acid glycoprotein 402 99.75
alpha-2-macroglobulin 116 99.14
peptides identified following abundant protein removal

Greater numbers of peptides identified following abundant protein removal. This proportional Venn diagram displays the relative number of unique peptides identified by mass spectrometry when human serum is depleted using the Pierce Top 2 or Top 12 columns (Cat. No. 85182 and 85165, respectively) compared to non-depleted human serum. A simple, fast depletion using the Top 12 columns doubled the number of unique peptides identified when compared to non-depleted human serum.