Abundant Protein Depletion for Mass Spectrometry
Depletion is used to reduce the complexity of biological samples such as serum, plasma, or biofluids, which contain high concentrations of albumin and immunoglobulins. These products utilize immunoaffinity techniques to enable the removal of the most abundant proteins, thus enhancing the detection of lower-abundance proteins in both discovery and targeted proteomic analyses.
- Efficient—kits remove > 95% of albumin and > 90% of IgG (Top 2), plus other abundant proteins (Top 12)
- Fast—spin column formats enable the processing of samples in less than an hour
- Economical—cost-effective spin columns are priced for single use
- Consistent—one-time use helps prevent abundant protein carryover and experimental variability
Choose the right abundant protein depletion product for your mass spec application
|Order now||Order now||Order now|
Pierce™ Top 2 Abundant Protein Depletion Spin Columns
Pierce™ Top 12 Abundant Protein Depletion Spin columns
|Proteins depleted||Albumin||Albumin, IgG||Albumin, IgG,
and 10 other proteins*
|Sample volume capacity||10–50 µL||10 µL (600 µg)||10 µL (600 µg)|
|Processing time||20–30 min||40–60 min||40–60 min|
|Format||Loose resin, spin columns, buffers||Pre-filled spin columns||Pre-filled spin columns|
*Top 12 proteins include albumin, IgG, apolipoprotein A-II, fibrinogen, α1-acid glycoprotein, haptoglobin, α1-antitrypsin, IgA, α2-macroglobulin, IgM, apolipoprotein A-I, transferrin
Video for abundant protein depletion
Featured product data
Protein removal achieved using Pierce™ Top 12 Abundant Protein Depletion Spin Columns (Cat. No. 85165). Values were determined by targeted mass spectrometry. The albumin depletion percentage was cross-validated by ELISA and was in agreement with >99% removal.
|Protein||Fold reduction||Percent depletion|
Greater numbers of peptides identified following abundant protein removal. This proportional Venn diagram displays the relative number of unique peptides identified by mass spectrometry when human serum is depleted using the Pierce Top 2 or Top 12 columns (Cat. No. 85182 and 85165, respectively) compared to non-depleted human serum. A simple, fast depletion using the Top 12 columns doubled the number of unique peptides identified when compared to non-depleted human serum.
For Research Use Only. Not for use in diagnostic procedures.