Clinical Gene Expression and Profiling
RNAlater® Tissue Collection: RNA Stabilization Solution is an aqueous tissue storage reagent that protects RNA within intact, unfrozen tissue samples. RNAlater Solution was designed to eliminate the need to immediately process tissue samples or to freeze samples in liquid nitrogen for later processing. This has proved advantageous in situations in which sample deep freezing methods are not convenient and sample numbers are large, e.g., when sampling a large number of patients in a clinical setting. In the studies described below, Drs. Cui and Gardner both use RNAlater Solution to store and stabilize clinical skin biopsy samples, utilizing gene expression analysis via real time RT-PCR and array analysis, respectively.
RNAlater® Solution Preserves Colorectal Biopsy Samples for Real-time PCR Studies
The integrity of RNA from samples stored for eight days (RIN=8.50 ± 0.42) in RNA later Solution was comparable to that from samples stored for just one hour (RIN = 9.04 ± 0.27) indicating that RNA remained undegraded during transport in RNA later Solution even after eight days at room temperature. The stability afforded by RNA later Solution improved the clinical use of real-time PCR for quantification of TNF-α gene expression in these colorectal biopsies.
RNAlater® Solution Stabilizes and Preserves Scleroderma Skin Samples for Gene Profiling with Affymetrix Arrays
The results from RNA later Solution-treated tissue indicated that scleroderma has a distinct gene profile that is incompletely reflected in profiles from fibroblast cultures. Results confirmed a constant yet different expression pattern across the controls, indicating that RNA later Solution can stabilize RNA for subsequent gene profiling. RNA later Solution provides flexibility for sample collection and storage, and helps ensure that high quality RNA is preserved in samples.
- Cui G, Olsen T, Christiansen I, Vonen B, Florholmen J and Goll R (2006) Improvement of real-time polymerase chain reaction for quantifying TNF-α mRNA expression in inflamed colorectal mucosa: An approach to optimize procedures for clinical use, Scand J Clin Lab Invest 66:249–260.
- Gardner H, Shearstone J, Bandaru R, Crowell T, Lynes M, Trojanowska M, Pannu J, Smith E, Jablonska S, Blaszczyk M, Tan F, and Mayes M (2006) Gene profiling of scleroderma skin reveals robust signatures of disease that are imperfectly reflected in the transcript profiles of explanted fibroblasts. Arthritis & Rheumatism 54:1961–1973.