- Purify total RNA and viral RNA from diverse sample types
- Obtain high RNA yields from a broad range of sample sizes
- Recover RNA samples in 20–50 µl
- Use manual or robotic sample processing
Magnetic beads offer many benefits for isolating RNA from cells and viral RNA from cell-free samples. For example, compared to glass fiber filter-based methods, Ambion’s MagMAX™ technology (patent pending) eliminates problems with filter clogging due to particulates in the samples. In addition, since only a small volume of magnetic beads is needed for high efficiency binding, the bound RNA can be eluted in just 20–50 µl of nuclease free water. This makes the MagMAX magnetic bead technology extremely useful for concentrating RNA from large, dilute samples.
The quality and purity of the eluted RNA is suitable for most common downstream applications, such as qRT-PCR, microarray analysis, Northern blotting, and ribonuclease protection assays (RPA).
Mammalian Cells and Tissues
All of the MagMAX Kits use a guanidinium thiocyanate-based solution to rapidly solubilize cellular membranes and simultaneously inactivate nucleases [1-2]. The protocol in the MagMAX-96 for Microarrays Total RNA Isolation Kit offers scientists the option of using TRI Reagent® (patented product) for sample lysis. To maximize efficiency and success, Ambion provides two alternative protocols for the kit. The “Spin” method has fewer steps, requires no DNase treatment, and allows for larger sample amounts (up to 20 mg) to be processed. The “No Spin” method can easily be integrated with a robotic liquid handler. This is the recommended kit for high throughput sample processing for microarray experiments. (Samples may be fresh or flash-frozen; the Spin method can be used with samples treated with RNAlater®.)