Organism TypeMouseATCC CRL-1934 and CRL-11632; pluripotent embryonic stem cells, full name is ES-D3 The cells spontaneously differentiate into embryonic structures in the absence of a feeder layer or conditioned medium. They can be maintained in the undifferentiated state by frequent subculture (every 2 to 3 days) on confluent feeder layers of irradiated (3000 rads) or mitomycin C treated (0.01 mg/ml for 90 minutes) primary mouse embryonic fibroblasts. Medium conditioned by Leukemia Inhibitory Factor (LIF) may also be used to maintain the undifferentiated state. LIF may also be added to ES-D3 cells cultured on feeder layers for additional support of the undifferentiated state.
ApplicationCell Culture / Growth Conditions,Transient Transfection

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