Next-Generation Sequencing (NGS)
Next-generation sequencing (NGS) is a high-throughput methodology that enables rapid sequencing of the base pairs in DNA or RNA samples. Supporting a broad range of applications, including gene expression profiling, chromosome counting, detection of epigenetic changes, and molecular analysis, NGS is driving discovery and enabling the future of personalized medicine.
Comprehensive solutions
NGS research areas and applications
Inherited Disease Research
From targeted gene panels to RNA expression and aneuploidy detection
HLA Typing
Genotyping Class I and Class II loci
Cancer Research
From basic to translational to clinical research
Human Identification (HID)
From genotyping to STRs to mitochondrial DNA
Reproductive Health Research
From pre-implantation to congenital research
Infectious Disease
From viruses to microbial communities
NGS Workflows & Data Management
Manage and track genomic data for NGS or Sanger sequencing in one platform.
Leading in NGS
Meet the new Ion GeneStudio S5 systems
Our latest NGS systems provide the versatility and scalability to handle multiple applications on a single platform. How can a single instrument scale so easily to accommodate multiple applications, throughputs, and data outputs? Watch the video to find out.
Targeted NGS performance of Oncomine Assays and NCI-MATCH Trial Assay
The Oncomine Comprehensive Assay and NCI-MATCH Trial Assay (based on the Oncomine Comprehensive Assay) have demonstrated robust performance even with very limited FFPE samples. See the data ›
New to Sanger or NGS?
Build your NGS knowledge in the Sequencing Education Center.
Featured application: liquid biopsy research
From sample to answer, Ion Torrent technology enables comprehensive solutions for analysis from cell-free DNA (cfDNA) and cell free RNA (cfRNA). Our liquid biopsy NGS portfolio now offers a new, broad, 52-gene pan-cancer assay
Check out our new cfDNA white paper that discusses the analytical methods used in verification of the Oncomine Lung cfDNA Assay with the Ion S5 XL System.
How does Ion Torrent NGS work?
Ion Torrent next-generation sequencing exploits the fact that addition of a dNTP to a DNA polymer releases a hydrogen ion. We measure the pH change resulting from those hydrogen ions using semiconductors, simultaneously measuring millions of such changes to determine the sequence of each fragment.
The semiconductor approach—unlike optics or modified nucleotides used in other NGS technologies—helps you implement a fast and simple workflow that scales to your research needs across multiple applications including inherited disease, oncology, infectious disease, human identification (HID), human leukocyte antigen (HLA) typing, and agrigenomics.
Our targeted sequencing approach introduces a PCR-based sequence enrichment step using Ion AmpliSeq technology that focuses on genes or even genetic variants of interest; a good example is the targeting of oncogenes and tumor suppressors in a cancer research study.
Support
Next-Generation Sequencing Support Center
Find tips, troubleshooting help, and resources for your next-generation sequencing applications.
For Research Use Only. Not for use in diagnostic procedures.
