With the CellInsight CX5 high-content instrument you can take advantage of the entire fluorescent spectrum to optimize your assay—and multiplex your components to ask more in-depth biological questions. Use the tables below to select reagents for each channel; they are divided into application areas, including products for segmentation, cell structure, and more.

  • Select the Fixed cells or Live/fixable cells tab to see a reagent selection that matches your detection protocol.
  • Click on individual product links (within colored cells) to see product details including spectral profiles, images, sample data sets, citations, and cell-based protocols.
  • Click on an application area to see selection guides for additional fluorescence-based tools and assays for imaging.
Blue
Green
Orange/Red
Deep Red
Near IR

Antibody labels for secondary detection

Alexa Fluor 350 Alexa Fluor 488 Alexa Fluor 555 Alexa Fluor 594 Alexa Fluor 647 Alexa Fluor 750
  Goat anti-chicken IgY Goat anti-chicken IgY Goat anti-chicken IgY Goat anti-chicken IgY  
Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG
Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG
Goat anti-rat IgG Goat anti-rat IgG Goat anti-rat IgG Goat anti-rat IgG Goat anti-rat IgG  
  Goat anti-guinea pig IgG Goat anti-guinea pig IgG Goat anti-guinea pig IgG Goat anti-guinea pig IgG  
  Goat anti-human IgG Goat anti-human IgG Goat anti-human IgG Goat anti-human IgG  
Donkey anti-goat IgG Donkey anti-goat IgG Donkey anti-goat IgG Donkey anti-goat IgG Donkey anti-goat IgG  
  Donkey anti-mouse IgG Donkey anti-mouse IgG Donkey anti-mouse IgG Donkey anti-mouse IgG  
  Donkey anti-rat IgG   Donkey anti-rat IgG    
  Rabbit anti-goat IgG Rabbit anti-goat IgG Rabbit anti-goat IgG Rabbit anti-goat IgG  
Rabbit anti-mouse IgG Rabbit anti-mouse IgG Rabbit anti-mouse IgG Rabbit anti-mouse IgG Rabbit anti-mouse IgG  
  Rabbit anti-rat IgG   Rabbit anti-rat IgG    
  Chicken anti-goat IgG   Chicken anti-goat IgG Chicken anti-goat IgG  
  Chicken anti-mouse IgG   Chicken anti-mouse IgG Chicken anti-mouse IgG  
  Chicken anti-rabbit IgG   Chicken anti-rabbit IgG Chicken anti-rabbit IgG  
  Chicken anti-rat IgG   Chicken anti-rat IgG Chicken anti-rat IgG  

Cell segmentation

Whole cell segmentation
HCS CellMask Blue HCS CellMask Green HCS CellMask Orange   HCS CellMask Deep Red  
Nuclear segmentation
HCS NuclearMask Blue SYTO 9 Green fluorescent nucleic acid stain SYTO 82 Orange fluorescent nucleic acid stain HCS NuclearMask Red HCS NuclearMask Deep Red  
DAPI SYTOX Green nucleic acid stain   HCS NuclearMask Red TO-PRO-3 stain  

Cell structure

Cytoskeleton—actin
Alexa Fluor 350 phalloidin Alexa Fluor 488 phalloidin Alexa Fluor 555 phalloidin Alexa Fluor 594 phalloidin Alexa Fluor 647 phalloidin  
Plasma membrane
Wheat germ agglutinin, Alexa Fluor 350 conjugate Wheat germ agglutinin, Alexa Fluor 488 conjugate Wheat germ agglutinin, Alexa Fluor 555 conjugate Wheat germ agglutinin, Alexa Fluor 594 conjugate Wheat germ agglutinin, Alexa Fluor 647 conjugate  
           
Blue
Green
Orange/Red
Deep Red

Cell segmentation

Plasma membrane segmentation
  CellMask Green plasma membrane stain CellMask Orange plasma membrane stain   CellMask Deep Red plasma membrane stain
Nuclear segmentation
HCS NuclearMask Blue SYTO 9 Green fluorescent nucleic acid stain SYTO 82 Orange fluorescent nucleic acid stain HCS NuclearMask Red HCS NuclearMask Deep Red

Cell tracking & tracing

CellTracker Blue CellTracker Green CellTracker Orange CellTracker Red CellTracker Deep Red

Cell structure

Cytoskeleton—tubulin
  Tubulin Tracker Green      
Endoplasmic reticulum
  ER-Tracker Green (BODIPY FL Glibenclamide)   ER-Tracker Red (BODIPY TR Glibenclamide)  
Lysosomes
      LysoTracker Red DND-99  
Mitochondria
    MitoTracker Orange CMTMRos MitoTracker Red CM-H2XRos MitoTracker Deep Red FM

Cell function probes

Cell viability
  Image-iT DEAD Green viability stain   Propidium iodide  
Cell proliferation
  Click-iT EdU Alexa Fluor 488 HCS Assay Click-iT EdU Alexa Fluor 555 HCS Assay Click-iT EdU Alexa Fluor 594 HCS Assay Click-iT EdU Alexa Fluor 647 HCS Assay
Apoptosis—caspase activity
  CellEvent Caspase-3/7 Green Detection Reagent      
Apoptosis—DNA fragmentation TUNEL
  Click-iT TUNEL Alexa Fluor 488 Imaging Assay   Click-iT TUNEL Alexa Fluor 594 Imaging Assay Click-iT TUNEL Alexa Fluor 647 Imaging Assay
Oxidative stress—general
  CellROX Green reagent     CellROX Deep Red reagent
Endocytosis—lysosome-directed
  pHrodo Green Dextran, 10,000 MW for endocytosis pHrodo Red Dextran, 10,000 MW for endocytosis    
  Dextran, Alexa Fluor 488, 10,000 MW Dextran, Alexa Fluor 555, 10,000 MW Dextran, Alexa Fluor 594, 10,000 MW Dextran, Alexa Fluor 647, 10,000 MW
Endocytosis—receptor-mediated
  Transferrin from human serum, Alexa Fluor 488 conjugate     Transferrin from human serum, Alexa Fluor 647 conjugate
Cell cycle
  Premo FUCCI Cell Cycle Sensor Premo FUCCI Cell Cycle Sensor    
Autophagy
  Premo Autophagy Tandem Sensor RFP-GFP-LC3B Premo Autophagy Tandem Sensor RFP-GFP-LC3B    

New protein synthesis

Click-iT HPG
  Alexa Fluor 488 Azide Alexa Fluor 555 Azide Alexa Fluor 594 Azide Alexa Fluor 647 Azide
Click-iT AHA
  Alexa Fluor 488 Alkyne Alexa Fluor 555 Alkyne Alexa Fluor 594 Alkyne Alexa Fluor 647 Alkyne
Click-iT OPP
  Alexa Fluor 488 Picolyl Azide Toolkit Alexa Fluor 555 Picolyl Azide Toolkit   Alexa Fluor 647 Picolyl Azide Toolkit
Blue
Green
Orange/Red
Deep Red
Near IR

Antibody labels for secondary detection

Alexa Fluor 350 Alexa Fluor 488 Alexa Fluor 555 Alexa Fluor 594 Alexa Fluor 647 Alexa Fluor 750
  Goat anti-chicken IgY Goat anti-chicken IgY Goat anti-chicken IgY Goat anti-chicken IgY  
Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG Goat anti-mouse IgG
Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG Goat anti-rabbit IgG
Goat anti-rat IgG Goat anti-rat IgG Goat anti-rat IgG Goat anti-rat IgG Goat anti-rat IgG  
  Goat anti-guinea pig IgG Goat anti-guinea pig IgG Goat anti-guinea pig IgG Goat anti-guinea pig IgG  
  Goat anti-human IgG Goat anti-human IgG Goat anti-human IgG Goat anti-human IgG  
Donkey anti-goat IgG Donkey anti-goat IgG Donkey anti-goat IgG Donkey anti-goat IgG Donkey anti-goat IgG  
  Donkey anti-mouse IgG Donkey anti-mouse IgG Donkey anti-mouse IgG Donkey anti-mouse IgG  
  Donkey anti-rat IgG   Donkey anti-rat IgG    
  Rabbit anti-goat IgG Rabbit anti-goat IgG Rabbit anti-goat IgG Rabbit anti-goat IgG  
Rabbit anti-mouse IgG Rabbit anti-mouse IgG Rabbit anti-mouse IgG Rabbit anti-mouse IgG Rabbit anti-mouse IgG  
  Rabbit anti-rat IgG   Rabbit anti-rat IgG    
  Chicken anti-goat IgG   Chicken anti-goat IgG Chicken anti-goat IgG  
  Chicken anti-mouse IgG   Chicken anti-mouse IgG Chicken anti-mouse IgG  
  Chicken anti-rabbit IgG   Chicken anti-rabbit IgG Chicken anti-rabbit IgG  
  Chicken anti-rat IgG   Chicken anti-rat IgG Chicken anti-rat IgG  

Cell segmentation

Whole cell segmentation
HCS CellMask Blue HCS CellMask Green HCS CellMask Orange   HCS CellMask Deep Red  
Nuclear segmentation
HCS NuclearMask Blue SYTO 9 Green fluorescent nucleic acid stain SYTO 82 Orange fluorescent nucleic acid stain HCS NuclearMask Red HCS NuclearMask Deep Red  
DAPI SYTOX Green nucleic acid stain   HCS NuclearMask Red TO-PRO-3 stain  

Cell structure

Cytoskeleton—actin
Alexa Fluor 350 phalloidin Alexa Fluor 488 phalloidin Alexa Fluor 555 phalloidin Alexa Fluor 594 phalloidin Alexa Fluor 647 phalloidin  
Plasma membrane
Wheat germ agglutinin, Alexa Fluor 350 conjugate Wheat germ agglutinin, Alexa Fluor 488 conjugate Wheat germ agglutinin, Alexa Fluor 555 conjugate Wheat germ agglutinin, Alexa Fluor 594 conjugate Wheat germ agglutinin, Alexa Fluor 647 conjugate  
           
Blue
Green
Orange/Red
Deep Red

Cell segmentation

Plasma membrane segmentation
  CellMask Green plasma membrane stain CellMask Orange plasma membrane stain   CellMask Deep Red plasma membrane stain
Nuclear segmentation
HCS NuclearMask Blue SYTO 9 Green fluorescent nucleic acid stain SYTO 82 Orange fluorescent nucleic acid stain HCS NuclearMask Red HCS NuclearMask Deep Red

Cell tracking & tracing

CellTracker Blue CellTracker Green CellTracker Orange CellTracker Red CellTracker Deep Red

Cell structure

Cytoskeleton—tubulin
  Tubulin Tracker Green      
Endoplasmic reticulum
  ER-Tracker Green (BODIPY FL Glibenclamide)   ER-Tracker Red (BODIPY TR Glibenclamide)  
Lysosomes
      LysoTracker Red DND-99  
Mitochondria
    MitoTracker Orange CMTMRos MitoTracker Red CM-H2XRos MitoTracker Deep Red FM

Cell function probes

Cell viability
  Image-iT DEAD Green viability stain   Propidium iodide  
Cell proliferation
  Click-iT EdU Alexa Fluor 488 HCS Assay Click-iT EdU Alexa Fluor 555 HCS Assay Click-iT EdU Alexa Fluor 594 HCS Assay Click-iT EdU Alexa Fluor 647 HCS Assay
Apoptosis—caspase activity
  CellEvent Caspase-3/7 Green Detection Reagent      
Apoptosis—DNA fragmentation TUNEL
  Click-iT TUNEL Alexa Fluor 488 Imaging Assay   Click-iT TUNEL Alexa Fluor 594 Imaging Assay Click-iT TUNEL Alexa Fluor 647 Imaging Assay
Oxidative stress—general
  CellROX Green reagent     CellROX Deep Red reagent
Endocytosis—lysosome-directed
  pHrodo Green Dextran, 10,000 MW for endocytosis pHrodo Red Dextran, 10,000 MW for endocytosis    
  Dextran, Alexa Fluor 488, 10,000 MW Dextran, Alexa Fluor 555, 10,000 MW Dextran, Alexa Fluor 594, 10,000 MW Dextran, Alexa Fluor 647, 10,000 MW
Endocytosis—receptor-mediated
  Transferrin from human serum, Alexa Fluor 488 conjugate     Transferrin from human serum, Alexa Fluor 647 conjugate
Cell cycle
  Premo FUCCI Cell Cycle Sensor Premo FUCCI Cell Cycle Sensor    
Autophagy
  Premo Autophagy Tandem Sensor RFP-GFP-LC3B Premo Autophagy Tandem Sensor RFP-GFP-LC3B    

New protein synthesis

Click-iT HPG
  Alexa Fluor 488 Azide Alexa Fluor 555 Azide Alexa Fluor 594 Azide Alexa Fluor 647 Azide
Click-iT AHA
  Alexa Fluor 488 Alkyne Alexa Fluor 555 Alkyne Alexa Fluor 594 Alkyne Alexa Fluor 647 Alkyne
Click-iT OPP
  Alexa Fluor 488 Picolyl Azide Toolkit Alexa Fluor 555 Picolyl Azide Toolkit   Alexa Fluor 647 Picolyl Azide Toolkit