Real-Time PCR Master Mixes for Gene Expression Analysis

Real-time PCR master mixes for gene expression analysis

Applied Biosystems qPCR and RT-qPCR master mixes are engineered to deliver exceptional specificity and reliability for your gene expression analysis. Broadly compatible and supported by trusted expertise, our master mixes are ideal for applications in infectious disease research, cancer research, biomarker discovery, and pharmacogenomics. Whether you need single-target SYBR green dye-based detection or advanced TaqMan probe-based multiplexing, our reagents enable fast, accurate, and reproducible results across a wide range of target concentrations. Accelerate your path to discovery with the accuracy and consistency you can trust.

TaqMan probe and SYBR Green qPCR master mixes

	TaqMan Fast Advanced Master Mix	PowerTrack SYBR Green Master Mix	PowerUp SYBR Green Master Mix
	Order now	Order now	Order now
Overview	Wide dynamic range and bench-top stability supports accurate quantification, even in duplex reactions	Universal 2X reagent master mix with built-in visual indicators for accurate reaction setup	Featuring 72-hour benchtop stability with a dual hot-start mechanism for excellent SYBR assay specificity
Chemistry	Probes, 2-step qPCR	SYBR Green dye, 2-step qPCR	SYBR green dye, 2-step qPCR
Instrument compatibility	Any qPCR instrument, from any manufacturer
Target	cDNA	cDNA	cDNA
Concentration	2X	2X	2X
Multiplexing	Up to 2 targets	No	No
Passive reference dye	Rox dye	Rox dye	Rox dye
Cycling mode: run time	Fast: ~40 min Standard: ~50 min	Fast: ~30 min Standard: ~60 min	Fast: ~30 min Standard: ~60 min
Carryover contamination control	Available with and without dUTP, UNG/UDG	Includes dUTP, UNG/UDG	Includes dUTP, UNG/UDG
Benchtop stability in preassembled reaction	72 hours	8 hours	72 hours
Sample tracking dye	No	Yes	No
Reverse transcriptase	SuperScript IV VILO Master Mix	SuperScript IV VILO Master Mix	High-Capacity RNA-to-cDNA Kit
Product page	TaqMan Fast Advanced Master Mix	PowerTrack SYBR Green Master Mix	PowerUp SYBR Green Master Mix

Other real-time PCR master mixes

Our master mixes have long supported researchers with their qPCR gene expression analysis. However, for optimal results we recommend upgrading to one of our newer master mixes listed in the table above, which offer superior performance.

Legacy TaqMan Master Mixes include:

Legacy SYBR Green master mixes include:

1-step RT-qPCR master mixes

	TaqMan Fast Virus 1-Step RT-qPCR Master Mix	TaqPath Duraplex 1-Step RT-qPCR Master Mix
	Order now	Order now
Overview	Highly sensitive, inhibitor-tolerant 1-step master mix that supports fast results with up to 4-target multiplexing	Featuring 27 benchtop stability, exceptional lot-to-lot consistency, and up to 6-target multiplex with minimal signal interference.
Chemistry	Probes, 1-step RT-PCR	Probes, 1-step RT-PCR
Instrument compatibility	Any qPCR instrument, from any manufacturer
Target	RNA	RNA
Concentration	4X	4X
Multiplexing	Up to 4 targets	Up to 6 targets
Passive reference dye	Available with and without Rox dye	Available with and without Rox dye
Cycling mode: run time	Fast: ~60 min Standard: ~90 min	Fast: ~60 min Standard: ~110 min
Product page	TaqMan Fast Virus 1-Step RT-qPCR Master Mix	TaqPath Duraplex 1-Step RT-qPCR Master Mix

Ordering information

TaqMan Fast Advanced Master Mix and reverse transcriptase

PowerTrack SYBR Green Master Mix and reverse transcriptase

PowerUp SYBR Green Master Mix and reverse transriptase

TaqMan Fast Virus 1-Step RT-qPCR Master Mix

TaqPath Duraplex 1-Step RT-qPCR Master Mix

Related products for gene expression analysis

Elevate qPCR performance and eliminate uncertainty with our high-performance products for every step of your workflow. Designed to work seamlessly with our master mixes, our sample preparation kits, reverse transcription reagents, and TaqMan Assays ensure high-quality results every time. Achieve reliable qPCR results with our comprehensive solutions

TaqMan Predesigned Gene Expression Assays

Choose from over 2.8 million predesigned assays covering more than 30 species to detect virtually any gene transcript with reliable precision.

Order TaqMan Assays

SuperScript VILO Master Mix for two-step RT-qPCR

SuperScript VILO Master Mix is a first-strand cDNA synthesis mix featuring Superscript IV Reverse Transcriptase for reliable synthesis and high cDNA yields.

Learn more

Gene expression resources and related pages

Specificity in gene expression analysis

Target specificity is a critical and sometimes challenging aspect of gene expression analysis. Without it, your data can be compromised, leading to inaccurate results and potentially flawed conclusions. Before running your next experiment, read our white paper and infographic to learn why specificity can be challenging in gene expression analysis. Review the role of qPCR detection chemistry in assay specificity and discover key insights to help you achieve reliably accurate results.

Download resources

Educational Resources for Gene Expression Analysis

Featured resources

For Research Use Only. Not for use in diagnostic procedures.