Figure 4. Fast cDNA synthesis capability. The Invitrogen Millennium RNA Marker was used in a 10 μL SuperScript IV RT reaction with oligo(dT) according to the product protocol. Alternative RTs were used according to their respective recommended protocols, except that reaction times were reduced to 10 min. First-strand cDNAs were resolved by alkaline gel electrophoresis, and cDNA was stained using SYBR Gold Nucleic Acid Gel Stain. During electrophoresis NaOH hydrolyzes all RNA, resulting in visualization of cDNA only.