Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Cell samples washed and concentrated using the Rotea instrument exhibit excellent viability and recovery in small volumes. T cell quality and composition are maintained after Rotea instrument processing, and PBMC isolation can be completed in much less time than was possible with density media protocols.
Input volume (L) |
Processing time (min) |
Cell recovery |
---|---|---|
T cell wash and concentrate (1x106 cells/mL) | ||
0.5 | 11 | >90% |
1.0 | 18 | |
3.0 | 38 | |
5.0 | 58 | |
10.0 | 114 | |
20.0 | 226 |
Input volume (L) |
Processing time (min) |
Cell recovery |
---|---|---|
PBMC isolation | ||
~0.25 (¼ Leukopak bag) | 25 |
>90% |
~0.5 (½ Leukopak bag) | 45 |
|
~1.0 (full Leukopak bag) | 85 |
|
5 | 116 |
|
10 | 230 |
Flow cytometry data shows that PBMC cell isolation with the CTS Rotea instrument taking less than 30 minutes produces similar results to a 2-hour Ficoll® media separation.
Flow cytometry data shows that CD4/CD8 T cell composition within the CD3⁺ total T cell population remains largely unchanged after washing and concentration with the CTS Rotea instrument.
T cell quality and composition after washing and concentration.
After PBMCs were isolated from healthy donors using the CTS Rotea System, the T cells were expanded in culture and assessed for CD4 and CD8 markers using flow cytometry. They were then washed and concentrated using the CTS Rotea System and assessed again. Comparison showed that CD4 and CD8 populations were consistent between the two analyses, demonstrating that the relative proportions were unaffected by CTS Rotea system processing.
For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. Caution: Not intended for direct administration into humans or animals.