Vanquish Online SPE HPLC and UHPLC Systems

Automated online sample preparation to save time and solvents.

Vanquish Online SPE HPLC and UHPLC Systems

Ensure confidence in complex sample analysis using the Thermo Scientific Vanquish Online SPE HPLC and UHPLC Systems. These systems perform analyte pre-concentration and sample clean-up using an automated, repeatable workflow while saving solvent and time compared to offline sample preparation steps. The system is configured using modules from the Vanquish Core HPLC, Vanquish Flex UHPLC or Horizon UHPLC systems, retaining all the performance, robustness and ease of use of the Vanquish LC platform.


Greater confidence

Automated online solid-phase extraction (SPE) provides maximum repeatability and confidence for challenging samples by trapping and enriching the analyte to remove unwanted sample matrix, such as salts and other contaminants, before loading onto the analytical column.


The modularity of the Vanquish LC systems makes it easy to build a system to best meet your application requirements with flexibility to later upgrade a standard HPLC instrument to a more advanced configuration for more complex analyses. 

Save time and resources

The automated workflow, with predefined trap and elution steps in the instrument method, allows for unattended sample analysis while reducing human error, saving time and solvent compared to offline approaches.

Easy and reliable tool-free connections 

Vanquish Online SPE HPLC and UHPLC systems are fitted with Thermo Scientific Viper fingertight fittings, which support near-zero dead volume connections and enable sharper peaks and tool-free setup.

CDS software

The Vanquish HPLC and UHPLC systems seamlessly integrate with our Thermo Scientific Chromeleon Chromatography Data System (CDS) to deliver superior compliance tools, networking capabilities, automation and data processing. 

What is online SPE for HPLC and UHPLC?

The online SPE system allows you to automate sample clean up to remove the sample matrix via a bidirectional trap column prior to being eluted for separation on the analytical column.


The flow scheme illustrates how the six-port valve is used to switch the trap column for sample loading and elution.


System specifications

The table below provides an overview of the Vanquish Online SPE HPLC and UHPLC system specifications for standard setups. Not all specifications are listed. Specifications may differ based on the specific system components selected. See detailed Component Specifications for more information.

Category Vanquish Online SPE HPLC and UHPLC Systems
System pressure, maximum 700, 1000 or 1500 bar, depends on system configuration
Flow range 0–5, 0-8 or 0-10 mL/min, depends on pumps selected
Pump options (default gradient delay volume)

Binary (35, 200, or 400 µL) 
Quaternary (679 µL) 

Dual (679 µL)  

Delay volume depends on pump(s) selected

Biocompatible Yes, depends on system configuration
pH range

2–12, chloride concentration up to 1 mol/L

System design Modular
Touchscreen user interface display compatible (optional) Yes, depends on system configuration
Solvent monitoring system compatible (optional) Yes, depends on system configuration
Customizable gradient delay volume to simplify method transfer Yes, depends on system configuration
SmartInject technology for maximum injection precision and improved column lifetime Yes
Injection volume range

Standard: Up to 0.01-25 or 0.01-100 µL (Vanquish Flex/Vanquish Horizon)  
Optional: Up to 0.01-100 or 0.01-1000 µL (Vanquish Core) Depends on sampler selected

Sample cooling

Yes, 4-40°C

Depends on system configuration

Sample capacity (standard) 
Based on 12 mm OD vials (≤1.5 mL) for comparison

4 racks of 54 vials (≤ 1.5 mL) each, other rack formats available 
+ capacity of 12 × 22.5 mm OD vials (≤ 10 mL) in the carousel
Sampler extension available (optional) Yes, with Vanquish Charger
Column compartment thermostatting

Yes, 5-120°C, depends on column compartment selected 
Still air and forced air

Column compartment pre-heater

Active and passive  
Depends on system configuration

Column compartment post-column cooler


Depends on column compartment selected

Detector choices

Vanquish Charged Aerosol Detector 

Vanquish Diode Array Detector 

Vanquish Fluorescence Detector 

Vanquish Multiple Wavelength Detector

Vanquish Variable Wavelength Detector

Refractive Index Detector

Mass Spectrometer

Component specifications

Standard Vanquish Online SPE HPLC and UHPLC systems are made up of the following components. Download the associated specifications sheet for more detail on each component. 

Contact us to get a quote or discuss an HPLC/UHPLC solution