Phase contrast images of Primary Human Mammary Epithelial Cells (HMEC) growing in culture
Day 1 - HMEC
Day 3 - HMEC
Day 5 - HMEC
Superior HMEC growth in serum-free HuMEC medium
Figure 1. HMEC grown in HuMEC Medium during first culture after thaw (left) and in continuous long-term culture (right) exhibit faster growth compared to a competitor’s HMEC culture system.
High-efficiency transduction with BacMam-enabled reagents
Using an optimized procedure (protocol available) HMEC can be transduced and will express exogenous proteins with > 95% efficiency using the BacMam system. The Premo™ Cameleon calcium sensor is a ratiometric calcium-sensitive fluorescent protein (cameleon) that is delivered using BacMam technology. Organelle Lights™ and Cellular Lights™ reagents combine BacMam technology in a ready to use format enabling expression of autofluorescent proteins that are directed to specific subcellular compartments and organelles via signal peptide or protein fusions (see figure below).
- Efficient delivery allows for large quantity of primary cells to be transduced
- Transduce in batch mode, aliquot, store frozen and use as needed
- Fluorescent protein expression unaffected by frozen storage
A. Premo™ Cameleon Calcium Sensor
B. Cellular Lights™ fluorescent proteins
C. Organelle Lights™ fluorescent proteins
Figure 2. HMEC were transduced with BacMam encoding proteins that enable robust visualization of distinct subcellular regions.
A. Premo™Cameleon Calcium Sensor (cat. P36207) shown in green, live cell image at 100X
B. Cellular Lights™ Actin-RFC (cat. C10127) shown in red, fixed cell image at 200X
C. Organelle Lights™ Golgi-RFP (cat. O10098) shown in red, fixed cell image at 200X
Note: Nuclei stained blue in panels B & C
For Research Use Only. Not for use in diagnostic procedures.