Mammary Epithelial Cell Culture Systems

Robust cell growth for your experiments

  • ≥500,000 cells/vial, cryopreserved at the end of 6’ culture
  • ≥70% viability
  • ≥16 population doublings
  • Superior growth rate over leading competitors
  • Expression of cytokeratin 5/6, 8, 18 and E-cadherin verified for each lot

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Phase contrast images of Primary Human Mammary Epithelial Cells (HMEC) growing in culture

HMEC grown in HuMEC medium with the addition of HuMEC growth supplement (included). Images of cells from first culture after thaw shown below.

Day 1 - HMEC



Day 3 - HMEC



Day 5 - HMEC

Superior HMEC growth in serum-free HuMEC medium

HuMEC medium is a serum-free medium engineered specifically for the culture of Human Mammary Epithelial Cells (HMEC). HuMEC medium supports superior cell growth in the first culture after thaw and throughout the guaranteed lifespan, when compared to a major competitors' culture system (Figure 1).

Figure 1. HMEC grown in HuMEC Medium during first culture after thaw (left) and in continuous long-term culture (right) exhibit faster growth compared to a competitor’s HMEC culture system.

High-efficiency transduction with BacMam-enabled reagents

Using an optimized procedure (protocol available) HMEC can be transduced and will express exogenous proteins with > 95% efficiency using the BacMam system. The Premo™ Cameleon calcium sensor is a ratiometric calcium-sensitive fluorescent protein (cameleon) that is delivered using BacMam technology. Organelle Lights™ and Cellular Lights™ reagents combine BacMam technology in a ready to use format enabling expression of autofluorescent proteins that are directed to specific subcellular compartments and organelles via signal peptide or protein fusions (see figure below).

  •  Efficient delivery allows for large quantity of primary cells to be transduced
  •  Transduce in batch mode, aliquot, store frozen and use as needed
  •  Fluorescent protein expression unaffected by frozen storage

A.  Premo™ Cameleon Calcium Sensor



B.  Cellular Lights™ fluorescent proteins



C.  Organelle Lights™ fluorescent proteins

Figure 2.  HMEC were transduced with BacMam encoding proteins that enable robust visualization of distinct subcellular regions.
A.  Premo™Cameleon Calcium Sensor (cat. P36207) shown in green, live cell image at 100X
B.  Cellular Lights™ Actin-RFC (cat. C10127) shown in red, fixed cell image at 200X
C.  Organelle Lights™ Golgi-RFP (cat. O10098) shown in red, fixed cell image at 200X
Note: Nuclei stained blue in panels B & C

Resources

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Resources

HMEC Technical White Paper

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