Gibco Zeocin is highly effective in a variety of organisms, including mammalian and insect cell lines, as well as in yeast, bacteria, and plants. As a member of the bleomycin family, Zeocin causes cell death by intercalating into and cleaving DNA.
Resistance to Zeocin is conferred by the Sh ble gene product, which binds the antibiotic and prevents it from binding DNA. This selection agent is effective in multiple cell types, so eukaryotic expression vectors only need to carry one drug selection marker. This reduces the overall size of the vector and makes subcloning and transfection easier and more efficient.
Table 1. Selection agents and resistant vectors.
|Cell types||Conc. (μg/mL)*||Invitrogen vectors carrying resistance marker|
|HeLa||~150||pcDNA4 vectors (Constitutive mammalian expression)|
|NIH3T3||~400||T-REx System, pcDNA4/TO vectors (Inducible mammalian expression)|
|CHO||~250||pSecTag2, pSecTag2/Hygro (Secreted mammalian expression)|
|COS-1||~400||ZeoCassette vectors (Constructing Zeocin-resistant vectors)|
|293 HEK||200–400||InsectSelect System, pIZ/V5-His vectors (Stable expression in insect cells)|
|Jurkat T-Cell||~200||pPICZ and pPICZ alpha vectors (Inducible expression in Pichia pastoris)|
|Sf9 insect||~250||pGAPZ and pGAPZ alpha vectors (Inducible expression in Pichia pastoris)|
* The optimal concentration for selection of your cell line should be determined using kill curves.