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MagMAX FFPE DNA/RNA Ultra Kit
Sequentially extract DNA and RNA into separate eluates from the SAME FFPE section
- Same yields you would expect from isolating DNA from fresh frozen blood
- Ideal for Oncomine Comprehensive and Oncomine Focus assays
- Easy to automate using KingFisher instruments
- Compatible with downstream applications like PCR and NGS, even when using FFPE tissue over 25 years old
- Contains Dynabeads MyOne Silane for consistent isolation of RNA, microRNA, and DNA
Automation Friendly
Use the MagMAX FFPE DNA/RNA Ultra kit with KingFisher instruments for a faster workflow suitable for downstream applications such as Oncomine Assays, PCR, and NGS.
Combine with AutoLys for a faster deparaffinization process
Speed up your deparaffinization process with the Applied Biosystems AutoLys system. Comprised of pretreated tubes and a rack system to reduce pipetting steps, the system eliminates the need for hazardous chemicals and minimizes loss of tissue sample.
Note: Not included with the Applied Biosystems MagMAX FFPE DNA/RNA Ultra Kit.
MagMAX FFPE DNA/RNA Ultra Kit
| Sample type | FFPE tissue samples, such as resections, biopsies, and aspirates |
| Sampel input volume | 5-40 μm standard input of slides or curls |
| Eution volume | 20-50 μL |
| Total processing time | 20 min for DNA, 48 min for RNA |
| Reactions (rxn) | 96 isolations (48 RNA and 48 DNA, or 96 DNA or 96 RNA) |
| Cat. No. | A31881 |
Read our application notes
How to use this kit with samples, such as solid tumor biopsies, core needle biopsies, and fine-needle aspirates and recover DNA, RNA, and microRNA suitable for downstream molecular applications, including qPCR and NGS, even when using FFPE tissue over 25 years old.
How the quality, purity, and yield obtained from FFPE samples is comparable to fresh-frozen blood (FF) counterparts.
Comparison of DNA and RNA from fresh-frozen vs. FFPE tissue samples
The magnetic bead–based technology in the new Applied Biosystems MagMAX FFPE DNA/RNA Ultra Kit allows sequential isolation of DNA and RNA from the same formalin-fixed, paraffin-embedded (FFPE) tissue sample (Figure 1 and 2). Recovering both DNA and RNA (including microRNA) from the same sample enables analysis and comparison of all the important biomarkers contained within your sample, such as hotspot mutations, copy number variations, gene fusions, and indels. The DNA and RNA are recovered in separate eluates—and both are compatible with a broad range of applications, including real-time PCR and next-generation sequencing (Figure 3 and 4).
Figure 1. DNA yields compared to column purification. DNA and RNA from consecutive 1 x 5 µm sections of four FFPE cancer tumor resection blocks were isolated with the MagMAX FFPE DNA/RNA Ultra Kit on the Thermo Scientific KingFisher Flex Purification System and with the Qiagen AllPrep kit. Samples were quantitated with Invitrogen Quant-iT DNAassay kit for DNA yield.
Figure 2. RNA yields compared to column purification. DNA and RNA from consecutive 1 x 5 µm sections of four FFPE cancer tumor resection blocks were isolatedwith the MagMAX FFPE DNA/RNA Ultra Kit on the Thermo Scientific KingFisher Flex Purification System and with the Qiagen AllPrep kit. RNA samples were quantitated with Invitrogen Quant-iT RNA assay kit for RNA yield.
Figure 3. Comparable or better targeted DNA sequencing results. The MagMAX FFPE DNA/RNA Ultra kit or a leading competitor’s kit was used to isolate both DNA and RNA from the same FFPE sections. DNA libraries were created using the Ion AmpliSeq Cancer Hotspot Panel v2, prepared using the Ion Chef System, and sequenced using the Ion PGM System. Data was analyzed using Torrent Suite Software.
Figure 4. Comparable or better RNA targeted sequencing results. The MagMAX FFPE DNA/RNA Ultra kit or a leading competitor’s kit was used to isolate both DNA and RNA from the same FFPE sections. RNA libraries were created using the Ion AmpliSeq RNA Cancer Panel, prepared using the Ion Chef System, and sequenced using the Ion PGM System. Data was analyzed using Torrent Suite Software.
MagMAX kits purify high-quality nucleic acids from cell-free samples suitable for a range of research applications, including next-generation sequencing (NGS), qPCR, and digital PCR.
| MagMAX Cell-Free Total Nucleic Acid Isolation Kit Purify free-circulating DNA, RNA and miRNA | MagMAX Cell-Free DNA Isolation Kit Isolate cell-free DNA in | |
 |  | |
| Sample volumes | 1.0–6 mL plasma | 100 µL–10 mL |
| Elution volumes | 15–60 μL | 15–50 µL |
| Time per run | 90 minutes total, 10–15 min hands-on time with KingFisher instruments | 45–60 min |
| Automation/HT |
|
|
| — | Learn more | |
| Order now | Order now |
Automation Friendly
Use the MagMAX FFPE DNA/RNA Ultra kit with KingFisher instruments for a faster workflow suitable for downstream applications such as Oncomine Assays, PCR, and NGS.
Combine with AutoLys for a faster deparaffinization process
Speed up your deparaffinization process with the Applied Biosystems AutoLys system. Comprised of pretreated tubes and a rack system to reduce pipetting steps, the system eliminates the need for hazardous chemicals and minimizes loss of tissue sample.
Note: Not included with the Applied Biosystems MagMAX FFPE DNA/RNA Ultra Kit.
MagMAX FFPE DNA/RNA Ultra Kit
| Sample type | FFPE tissue samples, such as resections, biopsies, and aspirates |
| Sampel input volume | 5-40 μm standard input of slides or curls |
| Eution volume | 20-50 μL |
| Total processing time | 20 min for DNA, 48 min for RNA |
| Reactions (rxn) | 96 isolations (48 RNA and 48 DNA, or 96 DNA or 96 RNA) |
| Cat. No. | A31881 |
Read our application notes
How to use this kit with samples, such as solid tumor biopsies, core needle biopsies, and fine-needle aspirates and recover DNA, RNA, and microRNA suitable for downstream molecular applications, including qPCR and NGS, even when using FFPE tissue over 25 years old.
How the quality, purity, and yield obtained from FFPE samples is comparable to fresh-frozen blood (FF) counterparts.
Comparison of DNA and RNA from fresh-frozen vs. FFPE tissue samples
The magnetic bead–based technology in the new Applied Biosystems MagMAX FFPE DNA/RNA Ultra Kit allows sequential isolation of DNA and RNA from the same formalin-fixed, paraffin-embedded (FFPE) tissue sample (Figure 1 and 2). Recovering both DNA and RNA (including microRNA) from the same sample enables analysis and comparison of all the important biomarkers contained within your sample, such as hotspot mutations, copy number variations, gene fusions, and indels. The DNA and RNA are recovered in separate eluates—and both are compatible with a broad range of applications, including real-time PCR and next-generation sequencing (Figure 3 and 4).
Figure 1. DNA yields compared to column purification. DNA and RNA from consecutive 1 x 5 µm sections of four FFPE cancer tumor resection blocks were isolated with the MagMAX FFPE DNA/RNA Ultra Kit on the Thermo Scientific KingFisher Flex Purification System and with the Qiagen AllPrep kit. Samples were quantitated with Invitrogen Quant-iT DNAassay kit for DNA yield.
Figure 2. RNA yields compared to column purification. DNA and RNA from consecutive 1 x 5 µm sections of four FFPE cancer tumor resection blocks were isolatedwith the MagMAX FFPE DNA/RNA Ultra Kit on the Thermo Scientific KingFisher Flex Purification System and with the Qiagen AllPrep kit. RNA samples were quantitated with Invitrogen Quant-iT RNA assay kit for RNA yield.
Figure 3. Comparable or better targeted DNA sequencing results. The MagMAX FFPE DNA/RNA Ultra kit or a leading competitor’s kit was used to isolate both DNA and RNA from the same FFPE sections. DNA libraries were created using the Ion AmpliSeq Cancer Hotspot Panel v2, prepared using the Ion Chef System, and sequenced using the Ion PGM System. Data was analyzed using Torrent Suite Software.
Figure 4. Comparable or better RNA targeted sequencing results. The MagMAX FFPE DNA/RNA Ultra kit or a leading competitor’s kit was used to isolate both DNA and RNA from the same FFPE sections. RNA libraries were created using the Ion AmpliSeq RNA Cancer Panel, prepared using the Ion Chef System, and sequenced using the Ion PGM System. Data was analyzed using Torrent Suite Software.
MagMAX kits purify high-quality nucleic acids from cell-free samples suitable for a range of research applications, including next-generation sequencing (NGS), qPCR, and digital PCR.
| MagMAX Cell-Free Total Nucleic Acid Isolation Kit Purify free-circulating DNA, RNA and miRNA | MagMAX Cell-Free DNA Isolation Kit Isolate cell-free DNA in | |
| | |
| Sample volumes | 1.0–6 mL plasma | 100 µL–10 mL |
| Elution volumes | 15–60 μL | 15–50 µL |
| Time per run | 90 minutes total, 10–15 min hands-on time with KingFisher instruments | 45–60 min |
| Automation/HT |
|
|
| — | Learn more | |
| Order now | Order now |
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