SARS-CoV-2

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Nucleic acid isolation kits and consumables for SARS-CoV-2 research

The following products—including KingFisher Flex Purification System and MagMAX Viral Pathogen Nucleic Acid Isolation Kits—are recommended for SARS-Cov-2 viral nucleic acid extraction.

The following products are recommended for SARS-CoV-2 viral nucleic acid isolation.

MagMAX Viral/Pathogen Nucleic Acid Isolation Kit

MagMAX Viral/Pathogen Nucleic Acid Isolation Kit (MVP I)

MagMAX Viral/Pathogen II Nucleic Acid Isolation Kit (MVP II)

Bulk reagents

Sample types

Universal viral transport media (VTM), bronchoalveolar lavage (BAL) and other respiratory research samples, urine, whole blood, and saliva

What are the differences?

Binding beads: In order to support the unprecedented demand for RNA extraction products for COVID-19 testing. There has been a raw material change in the bead component contained in the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit. The MagMAX Viral/Pathogen II Nucleic Acid Isolation Kit contains these beads. The chemistry driving the RNA to the beads is identical, and the nucleic acid binding capacity of each bead type is sufficient to provide similar results between the two products.

Bulk reagent format: The MagMAX Viral/Pathogen II kit is only available in bulk prep format with 1,000 or 2,000 reactions.

Bulk reagents can be purchased separately.

Cat. No.

A48310 (up to 2,000 preps/kit)
A42352 (up to 200 preps/kit)
A48383 (up to 2,000 preps/kit) A42359 (binding solution)
A42360 (wash solution) 
A42362 (binding beads)
A42363 (proteinase K)
A42364 (elution buffer)
KingFisher Flex

The KingFisher Flex Purification system is the most versatile benchtop automated extraction instrument in the lab­­­­ for processing 24–96 samples per run (<40 minutes). Experience consistent extraction and purification of RNA, DNA, protein and cells from your samples.

Thermo Scientific KingFisher plastics are ideal for magnetic particle processing due to their low binding affinity for biomolecules. Both the yield and the quality of the isolated DNA/RNA are significantly improved with special Thermo Scientific KingFisher plates and tips.

See how-to videos to learn how to use the MagMAX Viral/Pathogen Nucleic Acid Isolation kits with KingFisher Instruments and saliva collection devices.

How to use the MagMAX Viral/Pathogen Kit with the KingFisher Flex Purification System

Watch how to extract RNA for SARS-CoV-2 research and testing using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit with the KingFisher Flex Purification System for high-throughput nucleic acid isolation.

How to use the MagMAX Viral/Pathogen Kit with the KingFisher Duo Prime Purification System

Watch how to extract RNA for SARS-CoV-2 research and testing using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit with the KingFisher Duo Prime Purification System for medium-throughput nucleic acid isolation.

How to use the MagMAX Viral/Pathogen Kit using the manual extraction protocol for SARS-CoV-2 sample preparation

Watch how to extract RNA for SARS-CoV-2 research and testing using the MagMAX Viral/Pathogen kit using the manual extraction protocol for SARS-CoV-2 sample preparation.

How to use a Multidrop Combi Reagent Dispenser to prepare KingFisher plates for SARS-CoV-2 sample preparation

Watch how to use the Thermo Scientific Multidrop Combi Reagent Dispenser to prepare KingFisher Flex plates for MagMAX Viral/Pathogen Nucleic Acid Isolation of SARS-CoV-2.

How to use a Spectrum DNA SDNA-1000 saliva collection kit

Watch this video to learn techniques on how to collect saliva effectively for nucleic acid isolation using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit.

How to use a DNA Genotek OMNIgene ORAL (OM-505) collection kit

Watch this video to learn techniques on how to collect saliva effectively for nucleic acid isolation using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit.

How to use a 50 mL conical tube for saliva collection

Watch this video to learn techniques on how to collect saliva effectively for nucleic acid isolation using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit.

How to use a GeneFIX Saliva DNA/RNA collection device

Watch this video to learn techniques on how to collect saliva effectively for nucleic acid isolation using the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit.

Sample input volume reduction

The following protocols can be used for either the MagMAX Viral/Pathogen Kit (MVP I) and MagMAX Viral/Pathogen II Kit (MVP II) for SARS-CoV-2 testing, which supports sample input volume reduction of 400 μL to 200 μL, and reduction from 3 wash steps to 2 wash steps. Protocols can be downloaded for the KingFisher Flex 96 Deep-well Head (5400630).

Protocol type Standard wash (3 wash steps) Reduced wash (2 wash steps)
200 μL script MVP_Flex_200ul.bdz MVP_2Wash_200_Flex.bdz
400 μL script MVP_Flex_400ul.bdz MVP_2Wash_400_Flex.bdz

Saliva collection

The following protocol can be used for either the MagMAX Viral/Pathogen Kit (MVP I) and MagMAX Viral/Pathogen II Kit (MVPII) for SARS-CoV-2 testing, which supports the isolation of viral nucleic acid from 200 µL of saliva (raw or preserved).

Nucleic acid Sample type Kit name SKU Format Software protocol
RNA Saliva MagMAX Viral/Pathogen Kit (MVP I)
A48310
A42352
up to 2,000 preps
up to 200 preps
MagMAX Viral/Pathogen RNA Saliva
 Flex
MagMAX Viral/Pathogen II Kit (MVPII) A48383 up to 2,000 preps

The following products are recommended for SARS-CoV-2 viral nucleic acid isolation.

MagMAX Viral/Pathogen Nucleic Acid Isolation Kit

MagMAX Viral/Pathogen Nucleic Acid Isolation Kit (MVP I)

MagMAX Viral/Pathogen II Nucleic Acid Isolation Kit (MVP II)

Bulk reagents

Sample types

Universal viral transport media (VTM), bronchoalveolar lavage (BAL) and other respiratory research samples, urine, whole blood, and saliva

What are the differences?

Binding beads: In order to support the unprecedented demand for RNA extraction products for COVID-19 testing. There has been a raw material change in the bead component contained in the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit. The MagMAX Viral/Pathogen II Nucleic Acid Isolation Kit contains these beads. The chemistry driving the RNA to the beads is identical, and the nucleic acid binding capacity of each bead type is sufficient to provide similar results between the two products.

Bulk reagent format: The MagMAX Viral/Pathogen II kit is only available in bulk prep format with 1,000 or 2,000 reactions.

Bulk reagents can be purchased separately.

Cat. No.

A48310 (up to 2,000 preps/kit)
A42352 (up to 200 preps/kit)
A48383 (up to 2,000 preps/kit) A42359 (binding solution)
A42360 (wash solution) 
A42362 (binding beads)
A42363 (proteinase K)
A42364 (elution buffer)
KingFisher Flex

The KingFisher Flex Purification system is the most versatile benchtop automated extraction instrument in the lab­­­­ for processing 24–96 samples per run (<40 minutes). Experience consistent extraction and purification of RNA, DNA, protein and cells from your samples.

Thermo Scientific KingFisher plastics are ideal for magnetic particle processing due to their low binding affinity for biomolecules. Both the yield and the quality of the isolated DNA/RNA are significantly improved with special Thermo Scientific KingFisher plates and tips.

See how-to videos to learn how to use the MagMAX Viral/Pathogen Nucleic Acid Isolation kits with KingFisher Instruments and saliva collection devices.

How to use the MagMAX Viral/Pathogen Kit with the KingFisher Flex Purification System

Watch how to extract RNA for SARS-CoV-2 research and testing using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit with the KingFisher Flex Purification System for high-throughput nucleic acid isolation.

How to use the MagMAX Viral/Pathogen Kit with the KingFisher Duo Prime Purification System

Watch how to extract RNA for SARS-CoV-2 research and testing using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit with the KingFisher Duo Prime Purification System for medium-throughput nucleic acid isolation.

How to use the MagMAX Viral/Pathogen Kit using the manual extraction protocol for SARS-CoV-2 sample preparation

Watch how to extract RNA for SARS-CoV-2 research and testing using the MagMAX Viral/Pathogen kit using the manual extraction protocol for SARS-CoV-2 sample preparation.

How to use a Multidrop Combi Reagent Dispenser to prepare KingFisher plates for SARS-CoV-2 sample preparation

Watch how to use the Thermo Scientific Multidrop Combi Reagent Dispenser to prepare KingFisher Flex plates for MagMAX Viral/Pathogen Nucleic Acid Isolation of SARS-CoV-2.

How to use a Spectrum DNA SDNA-1000 saliva collection kit

Watch this video to learn techniques on how to collect saliva effectively for nucleic acid isolation using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit.

How to use a DNA Genotek OMNIgene ORAL (OM-505) collection kit

Watch this video to learn techniques on how to collect saliva effectively for nucleic acid isolation using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit.

How to use a 50 mL conical tube for saliva collection

Watch this video to learn techniques on how to collect saliva effectively for nucleic acid isolation using the MagMAX Viral/Pathogen Nucleic Acid Isolation kit.

How to use a GeneFIX Saliva DNA/RNA collection device

Watch this video to learn techniques on how to collect saliva effectively for nucleic acid isolation using the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit.

Sample input volume reduction

The following protocols can be used for either the MagMAX Viral/Pathogen Kit (MVP I) and MagMAX Viral/Pathogen II Kit (MVP II) for SARS-CoV-2 testing, which supports sample input volume reduction of 400 μL to 200 μL, and reduction from 3 wash steps to 2 wash steps. Protocols can be downloaded for the KingFisher Flex 96 Deep-well Head (5400630).

Protocol type Standard wash (3 wash steps) Reduced wash (2 wash steps)
200 μL script MVP_Flex_200ul.bdz MVP_2Wash_200_Flex.bdz
400 μL script MVP_Flex_400ul.bdz MVP_2Wash_400_Flex.bdz

Saliva collection

The following protocol can be used for either the MagMAX Viral/Pathogen Kit (MVP I) and MagMAX Viral/Pathogen II Kit (MVPII) for SARS-CoV-2 testing, which supports the isolation of viral nucleic acid from 200 µL of saliva (raw or preserved).

Nucleic acid Sample type Kit name SKU Format Software protocol
RNA Saliva MagMAX Viral/Pathogen Kit (MVP I)
A48310
A42352
up to 2,000 preps
up to 200 preps
MagMAX Viral/Pathogen RNA Saliva
 Flex
MagMAX Viral/Pathogen II Kit (MVPII) A48383 up to 2,000 preps

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