Choosing the correct first-strand cDNA synthesis kit is critical to obtaining high yields of quality, full-length cDNA that accurately represents the input RNA. The cDNA generated can be amplified using PCR, and can then be used in many downstream applications such as cloning and sequencing. Choose the SuperScript™ IV First-Strand cDNA Synthesis Kit for challenging RNA samples or the High-Capacity cDNA Reverse Transcription Kit for commonly used RNA templates. 

Which first-strand cDNA synthesis kit for RT-PCR is right for you?

Value choice for commonly used RNA templates Most effective cDNA synthesis kit for all RNA templates
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Optional 2 tubes
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  High-Capacity cDNA Reverse Transcription Kit

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SuperScript™ IV First Strand Synthesis System

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Reverse transcriptase MultiScribe™ MuLV SuperScript™ IV
Optimal reaction temperature 37°C 50–55°C
RT reaction time 50 min 10 min
Sensitivity (total RNA) 20 ng–2 µg 10 pg–5 μg
cDNA yield Fair High
Reduced RNase H activity No Yes
Resistance to reaction inhibitors None High
Protocol Download protocol Download protocol

What is the difference between first-strand and second strand cDNA synthesis?