First-Strand cDNA Synthesis Kit for RT-PCR
Choosing the correct first-strand cDNA synthesis kit is critical to obtaining high yields of quality, full-length cDNA that accurately represents the input RNA. The cDNA generated can be amplified using PCR, and can then be used in many downstream applications such as cloning and sequencing. Choose the SuperScript™ IV First-Strand cDNA Synthesis Kit for challenging RNA samples or the High-Capacity cDNA Reverse Transcription Kit for commonly used RNA templates.
Which first-strand cDNA synthesis kit for RT-PCR is right for you?
|Value choice for commonly used RNA templates||Most effective cDNA synthesis kit for all RNA templates|
Optional 2 tubes
|High-Capacity cDNA Reverse Transcription Kit
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|SuperScript™ IV First Strand Synthesis System
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|Reverse transcriptase||MultiScribe™ MuLV||SuperScript™ IV|
|Optimal reaction temperature||37°C||50–55°C|
|RT reaction time||50 min||10 min|
|Sensitivity (total RNA)||20 ng–2 µg||10 pg–5 μg|
|Reduced RNase H activity||No||Yes|
|Resistance to reaction inhibitors||None||High|
|Protocol||Download protocol||Download protocol|
What is the difference between first-strand and second strand cDNA synthesis?
Other cDNA synthesis kits for end-point PCR applications
For Research Use Only. Not for use in diagnostic procedures.