The Invitrogen Attune NxT Autosampler, an optional accessory for the both the Attune NxT and the original Attune flow cytometers, enables rapid processing of up to 384 samples. See how the Autosampler works with the Attune NxT flow cytometer by requesting an in-lab demo from one of our trained representatives.
The Attune NxT Autosampler, with intelligent probe design, is designed to minimize clogging and carryover (1.0%) (Table 1) and prevent damage to the instrument. Mixing by gentle aspiration helps to ensure sample homogeneity and to maintain cell viability (Table 2), and consistent results are observed regardless of whether the sample is delivered from a tube or from a plate using the Attune NxT Autosampler (Figure 1).
Table 1. Minimal carryover using the Attune NxT Autosampler. Jurkat cells at a concentration of 1 x 106 cells/mL were dispensed into a 96-well, v-bottom plate, sampled using the Attune NxT Autosampler, and analyzed on the Attune NxT flow cytometer at two sample rates. Percent sample carryover was calculated for each wash routine.
Number of washes and % carryover
High throughput mode
Table 2. Increasing the number of mixing cycles on the Attune NxT Autosampler does not adversely affect cell viability. Ammonium chloride–lysed whole blood (LWB) and NIH/3T3 (live/heat-treated) cells were stained with 2 μg/mL propidium iodide (a dead-cell indicator), loaded in a 96-well plate (in triplicate), mixed 0–5 times using the Attune NxT Autosampler, and analyzed on the Attune NxT flow cytometer.
Percent dead cells (%)
Number of mix cycles
Figure 1. Attune NxT Autosampler offers consistent results regardless of sampling method. Lysed whole blood was stained for CD45, CD4, and CD8 markers, aliquotted into both a tube and a plate, and analyzed on an Attune NxT flow cytometer. Minimal variation was observed between the sampling modes.
Setting up a plate sampling run
Setting up a multiwell plate sampling run using the Attune NxT software is simple. Using a virtual plate layout view, you can create compensation wells, define instrument settings, and identify samples. The software also allows you to define multiple experiments on a single plate and recover unused sample (for samples collected or acquired in tube mode only).
The intuitive software is easy to use and enables quick data analysis. For example, a heat-map view allows rapid screening and confirmation of samples while the instrument is acquiring data (Figure 2).
Figure 2. Attune NxT Autosampler heat map function identifies variation within a parameter across a 96-well plate. Live and heat-killed THP-1 cells were stained with propidium iodide and dispensed into a 96-well plate. Samples were applied to the Attune NxT flow cytometer using the Attune Nxt Autosampler. Heat map (indigo correlating with live cells and red with dead cells) and numeric overlay text provide real-time feedback on the assay being performed.