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Automated Plasmid Purification |
Automated plasmid purification workflow
Experience the future of automated maxi-scale plasmid DNA purification. Introducing the KingFisher PlasmidPro Maxi Processor, designed to help simplify the entire plasmid purification process—from sample loading to elution. Our instrument allows researchers to walk away, starting from the overnight bacterial culture to purified plasmid DNA in under 75 minutes with less than 5 minutes of hands-on time. Improve efficiency without compromising quality. Free your scientists from laborious, time-consuming manual plasmid purification methods.
The KingFisher PlasmidPro Maxi Processor is a benchtop system that yields advanced transfection-grade plasmid DNA, suitable for a range of applications including transfection, protein expression, gene therapy, DNA sequencing, and vaccine research. Scientists in biotech, biopharma, research institutions, service labs, and beyond—grow your culture, and our instrument will handle the plasmid purification with push-button simplicity.
Learn more about automated plasmid prep with the KingFisher PlasmidPro Maxi Processor
eBook: Free up your plasmid purification workflows
Discover how automated plasmid purification can free up your lab's workflows and significantly improve its efficiency, scalability, and consistency without compromising quality. Get your copy of our eBook to explore real-world insights, actionable strategies, and innovative products you need to future-proof your pDNA purification processes.
Advantages of automating plasmid DNA purification
There are several advantages of automating plasmid DNA isolation including saving time, improving efficiency, and increasing reliability of results. The KingFisher PlasmidPro system offers a range of key benefits for maxi-scale plasmid DNA purification. Enhance your productivity and performance in plasmid DNA purification with this user-friendly bench-top instrument. The system helps provide a seamless workflow and consistent results.
Help save time and improve efficiency
Compared to manual methods or process, automation helps reduce the time and labor required for plasmid isolation, allowing a 100% hands-free process with no centrifugation or pipetting.
Uses prefilled cartridges
Prefilled cartridges contain all necessary reagents. The KingFisher PlasmidPro system follows standardized protocols, minimizing variations and helping ensure reliable, consistent plasmid DNA quality and purity, suitable for a variety of applications.
Scale up production
Automation enables the generation of large quantities of high-quality plasmid DNA, essential for applications like recombinant protein production, therapeutic agents, or DNA vaccines.
Easy setup and operation
The system is easy to plug in, ready to use immediately, and suitable for users of all skill levels. Simply pour the culture and press the “Start run” button for easy plasmid purification.
"We are pioneering the development of programmable mRNA therapeutics, which hold the promise of more personalized and targeted treatments. Accelerating research and discovery is crucial for advancing the field of genetic medicine and ensuring that therapies are both effective and safe for specific tissue and cell types.
The Applied Biosystems MagMAX Pro HT NoSpin Plasmid MiniPrep Kit has been instrumental in our research due to its high throughput capabilities and consistent, reliable yields that have helped significantly streamline our plasmid DNA purification process. Not only has this kit enhanced our workflow efficiency, but also has empowered our team to focus on the innovative aspects of our research, accelerating our journey towards delivering groundbreaking mRNA therapies."
- Verified customer
Enhancing plasmid purification
Click image to enlarge
Plasmid DNA isolation is advancing towards automation. Thermo Fisher Scientific is helping lead this shift, enhancing purification with both automated and improved manual techniques. Our infographic explains how.
Simple automated plasmid purification workflow
The KingFisher PlasmidPro system offers a streamlined and user-friendly workflow for plasmid purification. Its simple design and intuitive interface make it easy to operate, even for users with minimal training. With automated steps and precise control, the KingFisher PlasmidPro system can help simplify the entire process, from sample loading to elution, helping ensure reliable and efficient plasmid purification every time.
Pour the culture
Insert the cartridge into the instrument and press "start"
Collect the tube with purified plasmid DNA in less than 75 minutes
Magnetic bead-based miniprep plasmid purification
Automated maxi-scale plasmid purification videos
Introducing the KingFisher PlasmidPro Maxi Processor
Unboxing the KingFisher PlasmidPro instrument
How to operate the KingFisher PlasmidPro system
How to collect pDNA and clean the KingFisher PlasmidPro instrument
Take a self-guided tour of the KingFisher PlasmidPro instrument
Virtually navigate the KingFisher PlasmidPro instrument to clearly see its features, reagents, and more!
Pre-filled cartridges for automated plasmid prep
The KingFisher PlasmidPro system helps provide users advanced transfection-grade pre-filled cartridges based on their specific downstream applications. This enables users to achieve the desired level of endotoxin removal, tailored to their requirements.
The endotoxin-free cartridges have a limit of <0.1 EU/µg pDNA.
Low volume automated plasmid isolation
The Applied Biosystems MagMAX Pro HT NoSpin Plasmid Miniprep Kit enables fast and scalable purification of transfection-grade plasmid DNA. Accelerate your plasmid purification process while helping reduce time and labor costs with this high‑throughput, advanced magnetic bead–based technology. Compatible for use with the versatile KingFisher sample purification systems, its features include:
- Fast—high-quality purified plasmid DNA in less than 35 minutes
- Efficient—yields up to 20 μg of high-copy pDNA per 1 mL of bacterial culture
- Easy to use—minimal hands-on steps utilizing dual magnetic beads
- High purity—delivers transfection-grade purification with very low endotoxin levels; no centrifugation or vacuum filtration required
- Automation-friendly—easily integrated into liquid handlers and compatible with the Thermo Scientific KingFisher Apex, Flex, and Presto systems
Analyze your pDNA with nanodrop technology
Analyze plasmid yield and purity with precision and speed using the Thermo Scientific NanoDrop Ultra Spectrophotometer and Fluorometer. With the inclusion of red and blue solid-state light-emitting diodes (LEDs), the NanoDrop Ultra FL and NanoDrop UltraC FL are capable of both micro-volume absorbance and fluorescence measurements.
KingFisher PlasmidPro processor performance data
Automated vs. manual plasmid DNA isolation and purification
The KingFisher PlasmidPro system consistently achieves high pDNA concentrations, exceptional elution volumes, and expected total yields, helping ensure reliable and reproducible results. Additionally, the system produces desired A260/A280 and A260/A230 purity ratio values, and preserves a high percentage of supercoiled form, indicating the integrity of the purified DNA. With its exceptional performance, the automated system is an excellent solution for researchers seeking efficient and high-quality maxi-scale plasmid DNA purification.
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` Click image to enlarge
Figure 1. Gel image and supercoiled determination of purified pDNA. (A) Representative samples from Plasmid C (6.6 kb) in lanes 1–15 were purified using the KingFisher PlasmidPro instrument and compared to pDNA from a manual column-based kit in lanes 16, 17, and 18 run in 1% agarose gel. L1 is an Invitrogen linearized dsDNA ladder and L2 is the supercoiled pDNA ladder. The gel image shows that the supercoiled pDNA is the predominant form at 6.6 kb. (B) Densitometry analysis was performed with purified pDNA samples run on an agarose gel using the iBright Imaging and Analysis Software. Data represents the percent supercoiled pDNA from a subset of samples analyzed using the KingFisher PlasmidPro Maxi Processor. The box plot represents the distribution of data. Each dot represents an individual purification process. N is the number of purified samples from each plasmid size. The results show an average supercoiled pDNA at >80% for all three plasmid sizes. The percent supercoiled is comparable to the manual process for each plasmid B, C, and D at 88%.
Common applications for maxi-scale plasmid purification
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| Applications | Protein expression Serves as a vector for expression and production of desired proteins | Viral vector Required for production of recombinant viral vectors (AAVs and LVs) | mRNA synthesis Utilized for development of mRNA therapeutics (IVT) | Cell and gene therapy Development of molecular tests to detect genes and mutations | Cloning A fundamental tool for gene cloning, gene expression, and genetic manipulation |
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| Product offerings |
GeneArt Gene and Protein Synthesis Services
From sequence to DNA or protein without lifting a pipette.
Automated plasmid purification resources
Resources
Nucleic Acid Purification and Analysis Support Center
Find tips, troubleshooting help, and resources for your nucleic acid purification & analysis applications.
- Protocol Videos—Videos to help you isolate nucleic acids using a variety of techniques.
- DNA & RNA Selection Guide—Find the right purification products.
- Videos—View our library of DNA & RNA purification and analysis videos.
- Application Notes—Explore our application notes from scientists sharing data for isolation products
For Research Use Only. Not for use in diagnostic procedures.
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