Prep sizes and purity grades for every application
Choose from our wide range of high-performing, cost-effective plasmid purification technologies designed to help you overcome common plasmid prep issues, such as low recovery and the presence of impurities.
Robust purification of plasmid DNA, in the amount and purity required for the downstream application of interest, is the key step of protein expression workflow. Learn more about how you can obtain transfection grade plasmid and use them with Gibco ExpiCHO and Expi293 transient mammalian expression systems.
Which plasmid DNA isolation technology is right for you?
|Rapid isolation of molecular biology quality||Gold standard for ultrapure,
|Enhanced purity for sensitive applications|
|Purity grade||Molecular||Transfection||Advanced transfection|
|Endotoxin||Standard (>10 EU/µg)||Low endotoxin (0.1–1 EU/µg)||Endotoxin-free
(< 0.1 EU/µg)
|Plasmid DNA purification kit||GeneJET||PureLink HiPure||PureLink Fast||PureLinkExpi
|Total protocol time||15–60 min||30–120 min||30 min||90–120 min|
|Prep size||Mini–Maxi||Mini–Giga||Midi, Maxi||Maxi, Mega, Giga|
|Yield up to||20 µg–1 mg||20 µg–15 mg||0.4 mg (midi prep) or 1.5 mg (maxi prep)||1.5–15 mg|
|Technology||Silica membrane||Anion exchange||Advanced silica||Anion exchange membrane|
|Order now||Order now||Order now||Order now|
How to do an endotoxin-free maxiprep
Watch this video on how to purify plasmid DNA using PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit. It will show you how to get fast, high quality, endotoxin-free plasmid DNA yields for sensitive applications like primary cell transfection.
How to purify molecular grade plasmid DNA
Learn how to purify molecular grade plasmid DNA in 15 minutes with the Thermo Scientific GeneJET Plasmid Miniprep Kit. Molecular grade plasmid DNA is suitable for cloning, nucleic acid labeling, PCR, sequencing and transformation.
How to do a megaprep plasmid DNA purification
Isolate transfection-grade plasmid DNA in as little as 90 minutes with the Invitrogen PureLink HiPure Expi Plasmid Megaprep Kit. The novel, proprietary anion-exchange resin in these kits combines large capacity with fast flow rate, high resolution, and efficiency.
How to purify transfection grade plasmid DNA
Learn how to purify transfection grade plasmid DNA with the Invitrogen PureLink HiPure Plasmid Filter Maxiprep Kit. Transfection grade plasmid DNA is suitable for all molecular-grade applications, as well as in vitro transcription and transfection.
Nucleic Acid Purification and Analysis Support Center
Find tips, troubleshooting help, and resources for your nucleic acid purification & analysis applications.
DNA purification and analysis guide
RNA isolation and purification guide
Advanced plasmid DNA purification: from transfectiongrade to ultraclean endotoxin-free preparations
PureLink Expi Endotoxin-Free Plasmid Purification Kits with new maxi size
For Research Use Only. Not for use in diagnostic procedures.