Plasmid Isolation

Isolate Plasmid DNA for Every Application

Our Invitrogen plasmid isolation kits offer options to eliminate the challenges raised by multi sample processing. We design our plasmid DNA extraction products to isolate plasmid DNA at the purity and scale you need. Choose from our wide range of high-performing, cost-effective plasmid purification technologies designed to help you overcome common plasmid prep issues, such as low recovery and the presence of impurities. Learn about how to purify plasmid DNA with Invitrogen PureLink and Thermo Scientific GeneJET extraction kits with our plasmid isolation protocol videos.

Robust purification of plasmid DNA, in the amount and purity required for the downstream application of interest, is the key step of protein expression workflow. Learn more about how you can obtain transfection grade plasmid and use them with Gibco ExpiCHO and Expi293 transient mammalian expression systems.

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Which plasmid DNA isolation and purification kit is right for you?

 Rapid isolation of molecular biology qualityGold standard for ultrapure,
high quality
Enhanced purity for sensitive applications
Purity gradeMolecularTransfectionAdvanced transfection
EndotoxinStandard (>10 EU/µg)Low endotoxin (0.1–1 EU/µg)Endotoxin-free
(< 0.1 EU/µg)
Plasmid DNA purification kitGeneJET
PureLink HiPure
PureLink HiPure
PureLink Fast
PureLink Fast
PureLinkExpi Endotoxin-Free
Total protocol time15–60 min30–120 min30 min90–120 min
Prep sizeMini–MaxiMini–GigaMidi, MaxiMaxi, Mega, Giga
Yield up to20 µg–1 mg20 µg–15 mg0.4 mg (midi prep) or 1.5 mg (maxi prep)1.5–15 mg
TechnologySilica membraneAnion exchangeAdvanced silicaAnion exchange membrane
ApplicationsNot sensitive
  • PCR
  • Cloning (digestion, ligation)
  • Sequencing
  • Transfection of standard, robust cell lines
  • All molecular biology applications
Very sensitive
  • Primary and stem cell transfection
  • Gene therapy and vaccine (in vivo) research
  • All standard transfection applications
  • All molecular biology applications
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Plasmid DNA Preparation Sizes

Overnight bacterial culture volume1–5 mL25–100 mL100–500 mL500 mL–2.5 L2.5–5 L
Approximate yieldUp to 40 µgUp to 400 µgup to 1.5 mgUp to 5 mgUp to 15 mg

Extract Plasmid DNA with PureLink and GeneJet

How to purify endotoxin-free plasmid DNA

Watch this video to learn how to purify plasmid DNA using the PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit. This video shows you how to isolate and purify high-quality, endotoxin-free plasmid DNA for sensitive applications like primary cell transfection.

How to purify molecular-grade plasmid DNA

This video shows you how to purify molecular-grade plasmid DNA in 15 minutes using our GeneJET Plasmid Miniprep Kit. Molecular-grade plasmid DNA is suitable for cloning, nucleic acid labeling, PCR, DNA sequencing, and transformation.

How to purify plasmid DNA using our megaprep kit

Isolate transfection-grade plasmid DNA in as little as 90 minutes with the Invitrogen PureLink HiPure Expi Plasmid Megaprep Kit. This innovative proprietary anion-exchange resin in these kits combines large capacity with fast flow rate, high resolution, and efficiency.

How to purify transfection grade plasmid DNA

Learn how to purify transfection grade plasmid DNA with the Invitrogen PureLink HiPure Plasmid Filter Maxiprep Kit. Transfection grade plasmid DNA is suitable for all molecular-grade applications, as well as in vitro transcription and transfection.

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For Research Use Only. Not for use in diagnostic procedures.