Plasmid Isolation

Prep sizes and purity grades for every application

Choose from our wide range of high-performing, cost-effective plasmid purification technologies designed to help you overcome common plasmid prep issues, such as low recovery and the presence of impurities.

Robust purification of plasmid DNA, in the amount and purity required for the downstream application of interest, is the key step of protein expression workflow. Learn more about how you can obtain transfection grade plasmid and use them with Gibco ExpiCHO and Expi293 transient mammalian expression systems.

Download DNA guideDownload Application note

Download DNA guide

Learn about all the DNA isolation technologies and solutions we offer.

Download DNA guide

Download DNA guide

Use this selection guide to choose the right plasmid DNA isolation kit.

Download Selection guide

Download DNA guide

See how our plasmid isolation kits and Gibco ExpiCHO/Expi293 platforms work together.

Download Application note

Which plasmid DNA isolation technology is right for you?

  Rapid isolation of molecular biology quality Gold standard for ultrapure,
high quality
Enhanced purity for sensitive applications
Purity grade Molecular Transfection Advanced transfection
Endotoxin Standard (>10 EU/µg) Low endotoxin (0.1–1 EU/µg) Endotoxin-free
(< 0.1 EU/µg)
Plasmid DNA purification kit GeneJET PureLink HiPure PureLink Fast PureLinkExpi
Endotoxin-Free
Total protocol time 15–60 min 30–120 min 30 min 90–120 min
Prep size Mini–Maxi Mini–Giga Midi, Maxi Maxi, Mega, Giga
Yield up to 20 µg–1 mg 20 µg–15 mg 0.4 mg (midi prep) or 1.5 mg (maxi prep) 1.5–15 mg
Technology Silica membrane Anion exchange Advanced silica Anion exchange membrane
Applications Not sensitive
  • PCR
  • Cloning (digestion, ligation)
  • Sequencing
Sensitive 
  • Transfection of standard, robust cell lines
  • All molecular biology applications
Very sensitive
  • Primary and stem cell transfection
  • Gene therapy and vaccine (in vivo) research
  • All standard transfection applications
  • All molecular biology applications
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Prep sizes

  Miniprep Midiprep Maxiprep Megaprep Gigaprep
Overnight bacterial culture volume 1–5 mL 25–100 mL 100–500 mL 500 mL–2.5 L 2.5–5 L
Approximate yield Up to 40 µg Up to 400 µg up to 1.5 mg Up to 5 mg Up to 15 mg

Videos

How to do an endotoxin-free maxiprep

Watch this video on how to purify plasmid DNA using PureLink Expi Endotoxin-Free Maxi Plasmid Purification Kit. It will show you how to get fast, high quality, endotoxin-free plasmid DNA yields for sensitive applications like primary cell transfection.

How to purify molecular grade plasmid DNA

Learn how to purify molecular grade plasmid DNA in 15 minutes with the Thermo Scientific GeneJET Plasmid Miniprep Kit. Molecular grade plasmid DNA is suitable for cloning, nucleic acid labeling, PCR, sequencing and transformation.

How to do a megaprep plasmid DNA purification

Isolate transfection-grade plasmid DNA in as little as 90 minutes with the Invitrogen PureLink HiPure Expi Plasmid Megaprep Kit. The novel, proprietary anion-exchange resin in these kits combines large capacity with fast flow rate, high resolution, and efficiency.

How to purify transfection grade plasmid DNA

Learn how to purify transfection grade plasmid DNA with the Invitrogen PureLink HiPure Plasmid Filter Maxiprep Kit. Transfection grade plasmid DNA is suitable for all molecular-grade applications, as well as in vitro transcription and transfection.

Resources