Environment Testing Solutions

Clearly, at this critical time, product safety and HACCP are as important as they have ever been, and in the current pandemic, food and beverage companies, retailers, public health authorities and other businesses are seeking solutions to monitor the presence of SARS-CoV-2 in their environments.

Studies have shown that in some conditions SARS-CoV-2 (the virus that causes COVID-19) can remain viable on common surfaces, such as that used for food packaging, for up to 28 days1. The virus may be transmitted when a healthy person touches contaminated food or environmental contact surfaces (including packaging materials), and then shortly afterwards, touches their eyes, mouth, or nose.

Proper cleaning, surveillance, and the prevention of cross-contamination are critical in the control of foodborne illnesses. The application of sound principles of environmental sanitation, personal hygiene and established food safety practices will reduce the likelihood that harmful pathogens including SARS-CoV-2 will threaten the safety of the food supply, regardless of how the food is sourced.

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Detection of SAR-CoV-2 from food packaging and environmental monitoring

Environment testing solutions

Use/Purpose

Description

Format

Order code

Surface sampling

 

Thermo Scientific™ MicroTest™ Flocked Swab Kits

Pack of 100 swabs

R12542

Swabs with synthetic tip and aluminum or plastic shaft (preferably large macrofoam tipped applicator)

Various

 

Go to fisherscientific.com

Transport medium

Thermo Scientific™ Phosphate Buffered Saline

24 x 20 mL BO0971G
100 tablets, each for 100 mL BR0014G

Use/Purpose

Description

Format

Order code

Medium to high throughput automated sample preparation

Thermo Scientific™ KingFisher™ Flex Purification System, with 96 Deep-well Head

Instrument

 

A32681

 

KingFisher™ Deep-Well 96 Plate, V-bottom 50 x 96-well plates 95040450
KingFisher™ Flex 96 Deep-Well Heating Block 1 block 24075430
KingFisher™ 96 tip comb for DW magnets Case of 100 97002534

Low to medium throughout automated sample preparation

Thermo Scientific™ KingFisher™ mL System Instrument 5400050C
Applied Biosystems™ KingFisher™ mL Food Protection Purification System Tubes & Tips 240 tests 15951
KingFisher™ mL Food Protection Purification System Tube Rack 1 rack 15952

Manual sample preparation

Invitrogen™ DynaMag™-2 Magnet

1 each

12321D

Nucleic acid extraction reagents for use with automated and manual extraction methods

Applied Biosystems™ PrepSEQ™ Nucleic Acid Extraction Kit for Food and Environmental Testing

300 preparations 4428176
100 preparations 4480466

Use/Purpose

Description

Format

Order code

Master Mix

Thermo Scientific™ RNA UltraSense™ One-Step Quantitative RT-PCR System

100 reactions

11732927

Total RNA Control (Human) 100 µL 4307281
Use/Purpose-TaqMan® PCR Assay TaqMan 2019nCoV Assay Kit v 1:   A47532
2019-nCoV (ORF1ab Assay, FAM dye, 20X 75 µL
2019-nCoV (S protein) Assay, FAM dye, 20X 75 µL
2019-nCoV (N protein) Assay, FAM dye, 20X 75 µL
RNase P Assay, VIC dye, 20X 250 µL
Synthetic positive control for assay performance verification TaqMan® 2019-nCoV Control Kit v1 2019nCoV DNA Control (covering Gene Orf-1ab, Gene S protein and Gene N protein, RNase P) 50 µL
(concentration 1 x 104 copies /µL)
A47533

Real-Time PCR and data analysis

Applied Biosystems™ QuantStudio™ 5 Food Safety Real-Time PCR System (96-well, 0.1mL Block)

Includes: Applied Biosystems™ QuantStudio™ Design and Analysis Software v1.5.1 or later and Thermo Scientific™ RapidFinder™ Analysis Software v.1.0 or later

System with laptop computer

A36328

Applied Biosystems™ 7500 Fast Food Safety Real-Time PCR System

Includes: Applied Biosystems™ 7500 Fast Instrument, RapidFinder™ Express v2.0 and SDS 1.4 or later software

System with laptop computer

A30299

The QuantStudio 5 and 7500 Food Safety Fast Real-Time PCR Systems recommended for use in the detection of SARS-CoV-2 when testing samples from food, packaging and the environment are also suitable for running tests for a broad range of other targets important to food safety and environmental testing. Detect foodborne pathogens using the Thermo Scientific™ SureTect™ Food Pathogen Detection System and benefit from simple, streamlined workflows and increased accuracy to confidently target Salmonella, Listeria, E. coli O157 and other STEC, Campylobacter, Cronobacter, Vibrio and Staphylococcus aureus.

Users will also have the capability to run other real-time PCR tests, including those in our portfolio for GMOs, animal species, Legionella and bio-threat organisms.

References

1.       Riddell, S., Goldie, S., Hill, A. et al. The effect of temperature on persistence of SARS-CoV-2 on common surfaces. Virol J 17, 145 (2020). https://doi.org/10.1186/s12985-020-01418-7 

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