Clearly, at this critical time, product safety and HACCP are as important as they have ever been, and in the current pandemic, food and beverage companies, retailers, public health authorities and other businesses are seeking solutions to monitor the presence of SARS-CoV-2 in their environments.
Studies have shown that in some conditions SARS-CoV-2 (the virus that causes COVID-19) can remain viable on common surfaces, such as that used for food packaging, for up to 28 days1. The virus may be transmitted when a healthy person touches contaminated food or environmental contact surfaces (including packaging materials), and then shortly afterwards, touches their eyes, mouth, or nose.
Proper cleaning, surveillance, and the prevention of cross-contamination are critical in the control of foodborne illnesses. The application of sound principles of environmental sanitation, personal hygiene and established food safety practices will reduce the likelihood that harmful pathogens including SARS-CoV-2 will threaten the safety of the food supply, regardless of how the food is sourced.
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Originally hosted by Select Science on April 20, 2021.
Listen to Danièle Sohier, senior manager, global scientific affairs – food production at Thermo Fisher Scientific, and Pat Bird, technical consultant at AOAC International, as they discuss the methods available to monitor SARS-CoV-2 in food production environments.
Thermo Scientific™ MicroTest™ Flocked Swab Kits
Pack of 100 swabs
Swabs with synthetic tip and aluminum or plastic shaft (preferably large macrofoam tipped applicator)
Go to fisherscientific.com
Thermo Scientific™ Phosphate Buffered Saline
|24 x 20 mL||BO0971G|
|100 tablets, each for 100 mL||BR0014G|
Medium to high throughput automated sample preparation
Thermo Scientific™ KingFisher™ Flex Purification System, with 96 Deep-well Head
|KingFisher™ Deep-Well 96 Plate, V-bottom||50 x 96-well plates||95040450|
|KingFisher™ Flex 96 Deep-Well Heating Block||1 block||24075430|
|KingFisher™ 96 tip comb for DW magnets||Case of 100||97002534|
Low to medium throughout automated sample preparation
|Thermo Scientific™ KingFisher™ mL System||Instrument||5400050C|
|Applied Biosystems™ KingFisher™ mL Food Protection Purification System Tubes & Tips||240 tests||15951|
|KingFisher™ mL Food Protection Purification System Tube Rack||1 rack||15952|
Manual sample preparation
Invitrogen™ DynaMag™-2 Magnet
Nucleic acid extraction reagents for use with automated and manual extraction methods
Applied Biosystems™ PrepSEQ™ Nucleic Acid Extraction Kit for Food and Environmental Testing
Thermo Scientific™ RNA UltraSense™ One-Step Quantitative RT-PCR System
|Total RNA Control (Human)||100 µL||4307281|
|Use/Purpose-TaqMan® PCR Assay||TaqMan 2019nCoV Assay Kit v 1:||A47532|
|2019-nCoV (ORF1ab Assay, FAM dye, 20X||75 µL|
|2019-nCoV (S protein) Assay, FAM dye, 20X||75 µL|
|2019-nCoV (N protein) Assay, FAM dye, 20X||75 µL|
|RNase P Assay, VIC dye, 20X||250 µL|
|Synthetic positive control for assay performance verification||TaqMan® 2019-nCoV Control Kit v1 2019nCoV DNA Control (covering Gene Orf-1ab, Gene S protein and Gene N protein, RNase P)||50 µL|
(concentration 1 x 104 copies /µL)
Real-Time PCR and data analysis
Applied Biosystems™ QuantStudio™ 5 Food Safety Real-Time PCR System (96-well, 0.1mL Block)
Includes: Applied Biosystems™ QuantStudio™ Design and Analysis Software v1.5.1 or later and Thermo Scientific™ RapidFinder™ Analysis Software v.1.0 or later
System with laptop computer
Applied Biosystems™ 7500 Fast Food Safety Real-Time PCR System
Includes: Applied Biosystems™ 7500 Fast Instrument, RapidFinder™ Express v2.0 and SDS 1.4 or later software
System with laptop computer
The QuantStudio 5 and 7500 Food Safety Fast Real-Time PCR Systems recommended for use in the detection of SARS-CoV-2 when testing samples from food, packaging and the environment are also suitable for running tests for a broad range of other targets important to food safety and environmental testing. Detect foodborne pathogens using the Thermo Scientific™ SureTect™ Food Pathogen Detection System and benefit from simple, streamlined workflows and increased accuracy to confidently target Salmonella, Listeria, E. coli O157 and other STEC, Campylobacter, Cronobacter, Vibrio and Staphylococcus aureus.
Thermo Fisher is at the heart of the global response to the COVID-19 pandemic. We are working with government agencies and researchers to ensure priority access to instruments, consumables, safety supplies and other products to address the outbreak–particularly in analysis of the virus, diagnosis and personal protection.
We know you rely on us as an essential partner to support your work now more than ever. Please visit our COVID-19 customer portal for more resources to support you.
1. Riddell, S., Goldie, S., Hill, A. et al. The effect of temperature on persistence of SARS-CoV-2 on common surfaces. Virol J 17, 145 (2020). https://doi.org/10.1186/s12985-020-01418-7