Flow cytometry analysis with a green or yellow laser
With the growing availability of flow cytometers equipped with green (532 nm) and yellow (561 nm) lasers, green light–excitable and yellow light–excitable fluorophores are playing an increasingly critical role in standard multiparametric flow cytometry. The expansive Alexa Fluor dye and eFluor dye portfolios support multicolor immunophenotyping by flow cytometry and includes a variety of dyes compatible with the 532 and 561 nm spectral lines of the green and yellow lasers, respectively.
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Properties of fluorescent dyes for green and yellow lasers
The table below lists the properties of the most commonly used fluorophores for green and yellow lasers.
|Fluorophore||Fluorophore type||Excitation max /Emission max (nm)||Relative intensity* (1 = lowest, 5 = highest)||Suggested bandpass filter (nm)||Uses|
|Alexa Fluor 532||Organic dye||532/554||3||555/20||Yellow-fluorescent dye with excitation ideally suited for the 532 nm laser|
|R-Phycoerythrin (R-PE, PE)||Protein||496, 565/575||5||575/26||Widely used fluorophore with a high quantum yield that is ideal for detection of low-density antigens|
|PE-Texas Red||Tandem||496, 565/615||3||590/40||Tandem dye, resonance energy transfer from PE molecule to Texas Red dye|
|PE-Alexa Fluor 610||Tandem||496, 565/628||3||590/40||Tandem dye, resonance energy transfer from PE molecule to Alexa Fluor 610 dye|
|PE-eFluor 610||Tandem||496, 565/607||3||610/20||Tandem dye, resonance energy transfer from PE molecule to eFluor 610; can be used as a brighter alternative to PE-Texas Red dye|
|PE-Cyanine 5 (TRI-COLOR)||Tandem||496, 565/667||3||670/14||Tandem dye, resonance energy transfer from PE molecule to the Cyanine 5 dye|
|PE-Cyanine 5.5||Tandem||496, 565/694||4||695/40||Tandem dye, resonance energy transfer from PE molecule to the Cyanine 5.5 dye|
|PE-Alexa Fluor 700||Tandem||496, 565/723||3||695/40||Tandem dye, resonance energy transfer from PE molecule to Alexa Fluor 700 dye|
|PE-Cyanine 7||Tandem||496, 565/767||4||780/60||Tandem dye, resonance energy transfer from PE molecule to Cyanine 7 dye|
|* The relative intensity of any fluorophore is dependent upon cell type being studied, reagent used, instrument and instrument configuration. The relative intensities provided are to be used as a guideline; observed intensities may vary.|
This online tool guides you through flow cytometry panel design, providing a simplified, customizable experience to fit your flow cytometry panel design needs.
Contact our technical sales specialists for help choosing antibodies, fluorophores, and reagents for success on the flow cytometer you’ll be using.
For Research Use Only. Not for use in diagnostic procedures.