Primary Antibodies for the Violet Laser

Detect more with a violet laser

Invitrogen eBioscience Super Bright polymer dyes are violet laser-excitable fluorophores that expand the capabilities of multi-parametric flow cytometry analysis. This family of bright fluorophores enables the use of more markers off the violet channel and increases the detection of low-abundance antigens due to their super bright signals. This empowers you to detect more in a single sample and answer complex cell biology questions—in less time and with less sample. 

The Super Bright polymer dyes join the trusted Invitrogen Alexa Fluor dye and Invitrogen eFluor dye portfolios in providing fluorochromes compatible with the 405 nm spectral line of the violet laser.

Find primary antibody conjugates

Step 1. Search for the marker of interest (e.g., “CD4” or “CD45”).
Step 2. Narrow results by conjugate, target species and other criteria using side filters. 

Super Bright polymer dyes

Super Bright dyes are a line of bright polymer fluorochromes excited by the violet laser (405 nm) to emit ultra-bright, spectrally tight emission spectrums. All Super Bright formats are named for their emission wavelength (Figure 1). Super Bright dyes are optimized for use in flow cytometry and have been shown to allow for better discrimination of dim populations due to their brightness. Furthermore, certain Super Bright exhibit less non-specific interaction with other polymer dyes as compared to other similar competitor polymer dye reagents. Learn more about Super Bright dyes.

Super Bright polymer dyes chart
 Figure 1. Emission spectra for Super Bright dyes.
Fluorescence intensity comparison

Figure 2. Fluorescence intensity comparison.(A) Human peripheral blood cells were stained with Anti-CD19 (clone HIB19) conjugated to either Super Bright 436 (purple histogram), eFluor 450 (blue histogram), or Brilliant Violet 421 dye (orange histogram) using the manufacturer’s recommended volume per test. (B) Anti-human peripheral blood cells were stained with Anti-CD27 (clone O323) conjugated to either Super Bright 436 (purple histogram) or Brilliant Violet 421 dye (blue histogram).

The Super Bright polymer dyes are fully compatible with other commonly used fluorescent molecules, Invitrogen eBioscience buffers and fixatives, and Invitrogen UltraComp eBeads microspheres. These features, combined with our broad portfolio of biological content, easily enable dye selection for optimized flow cytometry multicolor antibody panel design and allow you to expand the utility of your violet laser.

Properties of fluorescent dyes for the violet laser

The table below lists the properties of the most commonly used fluorophores for the violet laser.

FluorophoreFluorophore typeEx/Em (nm)Relative intensity* (1 = lowest, 5 = highest)Suggested bandpass filter (nm)Uses
Alexa Fluor 405Organic dye401/4211450/50Use this conjugate for detection of high-abundance targets
Super Bright 436Polymer dye414/4364450/50Use this conjugate for easier detection of low-abundance targets; use in place of Brilliant Violet 421 dye
eFluor 450Organic dye405/4502450/50Comparable to Pacific Blue dye
Pacific BlueOrganic dye410/4553450/50Comparable to eFluor 450
Pacific GreenOrganic dye410/5002522/31Use in place of AmCyan or Horizon V500
eFluor 506Organic dye419/5061510/50Use in place of AmCyan or Horizon V500
Pacific OrangeOrganic dye400 /5511585/40Orange-fluorescent dye; conjugates of this dye are fluorescent even at neutral pH
Super Bright 600Tandem polymer dye414/6005610/20Use this conjugate for easier detection of low-abundance targets; use in place of Brilliant Violet 605
Super Bright 645Tandem polymer dye414/6455660/20Use this conjugate for easier detection of low-abundance targets; use in place of Brilliant Violet 650 dye
Super Bright 702Tandem polymer dye414/7025710/50Use this conjugate for easier detection of low-abundance targets; use in place of Brilliant Violet 711 dye
Super Bright 780Tandem polymer dye414/7804780/60Use this conjugate for easier detection of low-abundance targets; use in place of Brilliant Violet 786 dye or Brilliant Violet 785 dye
Qdot 605NanocrystalUV—561/6053605/20Additional choice for multiplexed fluorescence detection
Qdot 655NanocrystalUV—633/6553655/20Additional choice for multiplexed fluorescence detection
Qdot 705NanocrystalUV—633/7053695/40Additional choice for multiplexed fluorescence detection
Qdot 800NanocrystalUV—633/8003780/60Additional choice for multiplexed fluorescence detection
* The relative intensity of any fluorophore is dependent upon cell type being studied, reagent used, instrument and instrument configuration. The relative intensities provided are to be used as a guideline; observed intensities may vary.

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Contact our eBioscience custom services group for antibody conjugation, antibody cocktails, immunoassay development, bulk reagents, and testing services.

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.