Primary Antibodies for the Violet Laser
Detect more with a violet laser
Invitrogen eBioscience Super Bright polymer dyes are violet laser-excitable fluorophores that expand the capabilities of multi-parametric flow cytometry analysis. This family of bright fluorophores enables the use of more markers off the violet channel and increases the detection of low-abundance antigens due to their super bright signals. This empowers you to detect more in a single sample and answer complex cell biology questions—in less time and with less sample.
The Super Bright polymer dyes join the trusted Invitrogen Alexa Fluor dye and Invitrogen eFluor dye portfolios in providing fluorochromes compatible with the 405 nm spectral line of the violet laser.
Step 1. Search for the marker of interest (e.g., “CD4” or “CD45”).
Step 2. Narrow results by conjugate, target species and other criteria using side filters.
Super Bright dyes are a line of bright polymer fluorochromes excited by the violet laser (405 nm) to emit ultra-bright, spectrally tight emission spectrums. All Super Bright formats are named for their emission wavelength (Figure 1). Super Bright dyes are optimized for use in flow cytometry and have been shown to allow for better discrimination of dim populations due to their brightness. Furthermore, certain Super Bright exhibit less non-specific interaction with other polymer dyes as compared to other similar competitor polymer dye reagents. Learn more about Super Bright dyes.
Figure 2. Fluorescence intensity comparison.(A) Human peripheral blood cells were stained with Anti-CD19 (clone HIB19) conjugated to either Super Bright 436 (purple histogram), eFluor 450 (blue histogram), or Brilliant Violet 421 dye (orange histogram) using the manufacturer’s recommended volume per test. (B) Anti-human peripheral blood cells were stained with Anti-CD27 (clone O323) conjugated to either Super Bright 436 (purple histogram) or Brilliant Violet 421 dye (blue histogram).
The Super Bright polymer dyes are fully compatible with other commonly used fluorescent molecules, Invitrogen eBioscience buffers and fixatives, and Invitrogen UltraComp eBeads microspheres. These features, combined with our broad portfolio of biological content, easily enable dye selection for optimized flow cytometry multicolor antibody panel design and allow you to expand the utility of your violet laser.
The table below lists the properties of the most commonly used fluorophores for the violet laser.
|Fluorophore||Fluorophore type||Ex/Em (nm)||Relative intensity* (1 = lowest, 5 = highest)||Suggested bandpass filter (nm)||Uses|
|Alexa Fluor 405||Organic dye||401/421||1||450/50||Use this conjugate for detection of high-abundance targets|
|Super Bright 436||Polymer dye||414/436||4||450/50||Use this conjugate for easier detection of low-abundance targets; use in place of Brilliant Violet 421 dye|
|eFluor 450||Organic dye||405/450||2||450/50||Comparable to Pacific Blue dye|
|Pacific Blue||Organic dye||410/455||3||450/50||Comparable to eFluor 450|
|Pacific Green||Organic dye||410/500||2||522/31||Use in place of AmCyan or Horizon V500|
|eFluor 506||Organic dye||419/506||1||510/50||Use in place of AmCyan or Horizon V500|
|Pacific Orange||Organic dye||400 /551||1||585/40||Orange-fluorescent dye; conjugates of this dye are fluorescent even at neutral pH|
|Super Bright 600||Tandem polymer dye||414/600||5||610/20||Use this conjugate for easier detection of low-abundance targets; use in place of Brilliant Violet 605|
|Super Bright 645||Tandem polymer dye||414/645||5||660/20||Use this conjugate for easier detection of low-abundance targets; use in place of Brilliant Violet 650 dye|
|Super Bright 702||Tandem polymer dye||414/702||5||710/50||Use this conjugate for easier detection of low-abundance targets; use in place of Brilliant Violet 711 dye|
|Super Bright 780||Tandem polymer dye||414/780||4||780/60||Use this conjugate for easier detection of low-abundance targets; use in place of Brilliant Violet 786 dye or Brilliant Violet 785 dye|
|Qdot 605||Nanocrystal||UV—561/605||3||605/20||Additional choice for multiplexed fluorescence detection|
|Qdot 655||Nanocrystal||UV—633/655||3||655/20||Additional choice for multiplexed fluorescence detection|
|Qdot 705||Nanocrystal||UV—633/705||3||695/40||Additional choice for multiplexed fluorescence detection|
|Qdot 800||Nanocrystal||UV—633/800||3||780/60||Additional choice for multiplexed fluorescence detection|
|* The relative intensity of any fluorophore is dependent upon cell type being studied, reagent used, instrument and instrument configuration. The relative intensities provided are to be used as a guideline; observed intensities may vary.|
For Research Use Only. Not for use in diagnostic procedures.