Invitrogen PrimeFlow RNA Assay expands the capability of flow cytometry to measure RNA. Combine PrimeFlow with immunolabeling of both cell-surface and intracellular proteins using fluorophore-conjugated antibodies. Researchers now can reveal the dynamics of RNA transcription together with protein expression patterns in single cell resolutions
Find PrimeFlow probes for your target–including SARS-CoV-2
Ready to start the PrimeFlow RNA Assay? Check what you’ll need
|Incubator||Capable of maintaining temperature at 40 ±1°C|
|Metal heat block||For 1.5 mL microcentrifuge tube, placed inside the validated incubator|
|Temperature validation kit||The Invitrogen ViewRNA Temperature Validation Kit uses a calibrated thermometer to verify the incubator temperature|
Optional: Is your target microRNA? This buffer helps ensure you get improved signal and better sensitivity for miRNA.
Optional: Will the assay be processed in a 96-well plate?
|Expression level of target RNA||Fluorochrome label||Laser excitation wavelength (nm)||Bandpass filter (nm)||Fluorescence channel||Probe set type|
|Low||Alexa Fluor 647||633, 635||660/20||APC, Alexa Fluor 647, eFluor 660||Type 1|
|Low||Alexa Fluor 568||561||610/20||PE-eFluor 610, Alexa Fluor 568, PE-Texas Red||Type 10|
|Medium-to-high||Alexa Fluor 488||488||530/30||FITC, Alexa Fluor 488||Type 4|
|Medium-to-high||Alexa Fluor 750||633, 635||780/60||APC-Cy7, APC-eFluor 780, Alexa Fluor 750||Type 6|
Use the step-by-step ordering information above to order your kit and probes, including controls. In step 4, if you need SARS-CoV-2 probes, see the table below, and follow the ‘How to order SARS-CoV-2 probes’ instructions provided.
|Dye||Type||Scale||Cat. No.||Assay ID|
PrimeFlow probe sets were designed using the NCBI strain and sequence from Accession Number LC521925, and cover multiple regions of the viral transcript. Find in the table below the binding sites and transcript region for the probe sets included in the assay. If you need a custom probe designed for a specific RNA region, use the Custom Branched DNA Probe Set Tool.
|Position (5' to 3')||Region|
|6||28212-29152||ORF8 and N|
Fluorophore and reagent selection guide for flow cytometry
Download Flow Cytometry Protocols Handbook
Spectral Flow Cytometry Fundamentals
Invitrogen eBioscience Resources—Selection guides, Best Protocols, product performance and more.
Intracellular Staining for Flow Cytometry How-To Video—for detecting cytokines and intranuclear markers.
Flow Cytometry Learning Center—Access flow cytometry educational resources for better experiment planning and execution.
Flow Cytometry Panel Builder—Design your flow cytometry panel with this online tool for a simplified, customizable experience to fit your needs.
Flow Cytometry Support Center—Find technical support recommendations for your flow cytometry workflows, including tips for experimental setup and in-depth troubleshooting help.
Flow Cytometry Panel Design Support—Work with one of our technical sales specialists to discuss your experimental needs and guide you through the process.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
For Research Use Only. Not for use in diagnostic procedures.