cancer immunity cycle schematic showing multistep anti-tumor responses

Flow cytometry is a powerful tool in the study of many areas of biology. In this subtopic section you will find educational assets that include application notes, selection guides, videos, webinars, tutorials and other tools related to flow cytometry and its use in immunology, immuno-oncology, cancer, and stem cell research.

Areas of biology features

Research overview

drawing of immuno-oncology-related cell types

Immuno-oncology research using flow cytometry

Learn how flow cytometry analysis is used in immuno-oncology research areas, including investigations into the tumor microenvironment, immune checkpoint analysis, and CAR T characterization.

BioProbes article

mastering technical aspects of flow cytometry infographic

A comprehensive resource for state-of-the-art flow cytometry methods

This article provides a review of recently published and extensive guidelines for the use of flow cytometry and cell sorting in immunological studies, as well as an infographic giving tips for key data controls.


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Type Title Categories
Application note (2017)Flow cytometry analysis of transcription factor expression during differentiation of hPSC-derived cardiomyocytesAttune/Attune NxT Flow Cytometer, cell differentiation, flow cytometry, multicolor flow cytometry, stem cell research
Application note (2017)Validated 10-color T cell panel using the Attune NxT Flow Cytometerantibodies, Attune/Attune NxT Flow Cytometer, flow cytometry, immunology, immunophenotyping, fluorescent dyes, multicolor flow cytometry
Application note (2015) Live/dead algal analysis with the Attune NxT Flow Cytometer algae, Attune NxT, flow cytometer, flow cytometry assays, fluorescent dyes, microbiology, viability
BioProbes article (2018)Immuno-oncology: Advances in Basic Research and Translational Medicine. With a focus on immune checkpoint inhibitors and T cell immunotherapyantibodies, ELISA, flow cytometry, fluorescence microscopy/fluorescence imaging, high content analysis (HCA), immunohistochemistry (IHC), immunology, immuno-oncology, immunophenotyping, Luminex, ProCartaPlex, spheroids
BioProbes article (2018)Flow cytometric assay for simultaneous assessment of HIV RNA and Gag protein. Single-cell characterization of viral translation-competent reservoirs in HIV-infected individuals antibodies and proteins, flow cytometry, HIV, immunology, in situ hybridization, PrimeFlow, protein expression, RNA detection
BioProbes article (2018)A comprehensive resource for state-of-the-art flow cytometry methods. Guidelines for the use of flow cytometry and cell sorting in immunological studiesantibodies and proteins, cell counting, cell cycle, cell proliferation, flow cytometer calibration, flow cytometry, flow cytometry compensation, flow cytometry sample preparation, immunology, immunophenotyping, viability
BioProbes article (2017)Harness immune checkpoints to combat tumors—Immune checkpoint antibodies for flow cytometry, IHC, and functional bioassaysantibodies, cancer, flow cytometry, immunofluorescence (IF), immunohistochemistry (IHC)
BioProbes article (2017)Powering up drug discovery—Cancer biology applications using the Attune NxT Flow Cytometerapoptosis, Attune NxT, cancer, flow cytometer, fluorescence, multicolor flow cytometry, viability
BioProbes article (2016)High-speed cell counting with the Attune NxT Flow Cytometer—Accurately measure cell concentrations by flow cytometryAttune NxT, bacteria, cell concentration, cell counting, flow cytometer, immunophenotyping, microbiology, viability
BioProbes article (2016)A complete suite of reagents, from cas9 delivery tools to cell function Assays—The CRISPR-cas9 system for genome editingCRISPR, flow cytometry assays, fluorescence, fluorescence microscopy/fluorescence imaging, high content analysis, western blotting
BioProbes article (2016)Transcription factor expression during differentiation of hPSC-derived cardiomyocytes—A multiparametric approach using the Attune NxT Flow CytometerAttune NxT, flow cytometer, fluorescence, large cell analysis, rare event detection, stem cell research
BioProbes article (2015)Tools and strategies for rare-event detection using flow cytometryAttune NxT, flow cytometer, fluorescence, rare cell detection, rare event detection
BioProbes article (2009) A flow cytometry–based method for isolating neural stem cells Alexa Fluor, cell isolation, flow cytometry, fluorescent dyes, neuroscience, stem cell research
BioProbes article (2008) Putting regulatory T cells to work—Isolate and expand CD4+ CD25+ Treg cells using Dynabeads magnetic separation technology cell expansion, cell isolation, flow cytometry, fluorescent dyes, immunology, magnetic beads, reagents
BioProbes article (2007) The latest in stem cell research antibodies, antibody labeling, cell differentiation, cell expansion, flow cytometry, fluorescent dyes, growth factors, fluorescence microscopy/fluorescence imaging, stem cell research
Guided learningT cell stimulation and proliferation eLearning courseantibodies, cell proliferation, cell stimulation, flow cytometry, immunoassays, immunology, immunophenotyping, T cells
Immunology informationAdaptive immunity overviewimmunology
Immunology informationB cell activation functional assayimmunology
Immunology informationCostimulatory and inhibitory interactions control T cell responseimmunology, stimulation, T cells
Immunology informationFoxp3 overviewflow cytometry, Foxp3, intracellular staining
Immunology informationInnate immunity overviewimmunology
Immunology informationInnate lymphoid cells overviewimmunology
Immunology informationMicrobiome (Microbiota) overviewimmunology
Immunology informationNatural killer cells overviewimmunology
Immunology informationT cell activation functional assayimmunology
Immunology informationT follicular helper differentiation functional assayimmunology
Immunology informationT reg differentiation functional assayimmunology
Immunology informationT regulatory cells overviewimmunology
Immunology informationTh1 differentiation functional assayimmunology
Immunology informationTh17 cells overviewimmunology
Immunology informationTh17 differentiation functional assayimmunology
Immunology informationTh2 differentiation functional assayimmunology
Immunology informationTh22 differentiation functional assayimmunology
Immunology informationTh9 differentiation functional assayimmunology
Immunology informationToll-like receptor overviewimmunology
Molecular Probes HandbookMHC tetramer technology—Note 7.2antibodies, flow cytometry, immunology, Major Histocompatibility Complexes, T cells
PathwaysAdaptive immunity pathwaysimmunology
PathwaysInnate immunity pathwaysimmunology
Pathways Inflammatory response pathwayimmunology
PathwaysInterferon (IFN) cell signaling pathwayimmunology
Pathways T-cell receptor (TCR) cell signaling pathwayimmunology
PathwaysToll-like receptor (TLR) cell signaling pathwayimmunology
Scientific poster (2018) Induced pluripotent stem cell sorting, culture and differentiation to desired cell lineage Attune/Attune NxT, cell counting, cell sorting, Countess, EVOS, fluorescence activated cell sorting, iSort Automated Cell Sorter, imaging microscopy, stem cell research
Scientific poster (2018) Modular, defined assembly of antibody-based constructs using click chemistry Alexa Fluor, antibody labeling, Attune/Attune NxT, CellInsight, drug discovery, drug research, high content analysis (HCA), SiteClick, spheroids
Scientific poster (2018) Prospective cytometric study evaluating the alkaline phosphatase activity as a potential functional biomarker of primitive malignant cells in acute leukemia antibodies, Attune/Attune NxT, cancer, cell function, flow cytometry, immunophenotyping, stem cell research
Scientific poster (2018) Synergistic effect of curcumin in combination with proapoptotic drugs in human cell models for chronic (CLL) lymphocytic leukaemia Attune/Attune NxT Flow Cytometer, cell cycle, cancer, drug development, drug research, flow cytometry
Scientific poster (2018) Differential expression of PD-L1 on myeloid derived suppressor cells as a potential screening approach for cancer immunotherapy in multiple myeloma Attune/Attune NxT, cancer, drug research, , immunology, immuno-oncology, multicolor flow cytometry, translational research solutions
Scientific poster (2018) Fluorescent hypoxia sensor for flow cytometry apoptosis, Attune/Attune NxT, brightfield microscopy, EVOS, flow cytometry, hypoxia, immunology
Scientific poster (2011) The next step in the analysis of lymphocyte proliferationAlexa Fluor, antibodies, Attune NxT, cell proliferation, CellTrace, flow cytometry assays, fluorescent dyes, live-cell imaging, violet laser-excited reagents
Scientific poster (2010) A comparison of three techniques to induce efficient ex vivo T cell expansion Alexa Fluor, antibodies, cell proliferation, CellTrace, flow cytometry, fluorescent dyes, immunology, magnetic beads, multicolor flow cytometry, viability
Scientific poster (2010) Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry and imagingAlexa Fluor, cell proliferation, cells, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF), immunophenotyping, stem cell research, violet laser-excited reagents
Scientific poster (2009) A novel xeno-free and serum-free culture medium for the expansion of human mesenchymal stem cells cell culture, cell expansion, flow cytometry, fluorescent dyes, gene expression, immunophenotyping, stem cell research
Scientific poster (2009) Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry Alexa Fluor, cell proliferation, cells, Click-iT, flow cytometry, fluorescent dyes, fluorescent proteins, immunophenotyping, nucleic acid quantitation, stem cell research, violet laser-excited reagents
Selection guideCytokine bioassays guidecytokines, flow cytometry, immunophenotyping, intracellular staining
Selection guideHuman CD & other cellular antigensflow cytometry, immunology, immunophenotyping, extracellular staining
Selection guideMouse CD & other cellular antigensflow cytometry, immunology, immunophenotyping, extracellular staining
CYToMORE lecture video (2016) Assessing immunological aging using flow cytometry 
Presented by Victor Appay, Faculté de Médecine Pierre et Marie Curie, Pitié-Salpêtrière, France
flow cytometry, immunology, immunophenotyping
CYToMORE lecture video (2016) Asymptomatic but threatening: dissecting the consequences of cytomegalovirus infection 
Presented by Anis Larbi, Singapore Immunology Network – SigN, Singapore
flow cytometry assays, immunology
CYToMORE lecture video (2016) Cancer stem cells and multi-drug resistance by flow cytometry 
Presented by Jordi Petriz, Josep Carreras Leukaemia Research Institute, IJC, Spain
cancer, flow cytometry, multidrug resistance (MDR), stem cell research
CYToMORE lecture video (2016) Cytometric analysis of cytokines and transcription factors 
Presented by Hyun-Dong Chang, Deutsches Rheuma-Forschungszentrum, Berlin, Germany
cell signaling, flow cytometry, immunophenotyping
CYToMORE lecture video (2016) Cytometry of immunity 
Presented by Andreas Radbruch, Deutsches Rheuma-Forschungszentrum Berlin, Germany
flow cytometry, immunology
CYToMORE lecture video (2016) Cytomic analysis of oxidative stress Presented by J. Enrique O’Connor, University of Valencia, Spain
flow cytometry, oxidative stress
CYToMORE lecture video (2016) Heterogeneity of tumour-infiltrating leukocytes: implications for adoptive cell transfer immunotherapy 
Presented by Enrico Lugli, Humanitas Clinical and Research Center, Italy
flow cytometry, immunology, immunophenotyping
CYToMORE lecture video (2016) Host-microbe interaction at mucosal surfaces 
Presented by Maria Rescigno, Istituto Europeo di Oncologia – IEO, Italy
flow cytometry, immunology, microbiology
CYToMORE lecture video (2016) Mass cytometry in the phenomics era: an essential tool for gene/function studies 
Presented by Herve Luche, Centre d’Immunophénomique - CIPHE, France
mass cytometry, immunology, immunophenotyping
CYToMORE lecture video (2016) Microenvironment cytometry 
Presented by Paul Smith, Cardiff University, UK
cancer, cell cycle, flow cytometry, tumor microenvironments, drug discovery
CYToMORE lecture video (2016) Rare cells analysis: from bench to clinics 
Presented by Andrea Cossarizza, University of Modena and Reggio Emilia, Italy
Attune NxT, flow cytometer, rare cell detection, rare event detection
Technology videoThe Two Worlds of Cell Separationcell isolation, immunology
WebinarThe Complex Pharmacology of T-Cell CARs 
In this webinar, Charles Prussak, PharmD, PhD., Director of the Cell Therapy Translational Laboratory (CTTL), UCSD, discusses the generation CAR-T therapies, toxicities that are most commonly observed when employing CD-19 directed CAR-T therapies, and the next generation of CAR-T cells being created and clinically tested for the treatment of both hematological and solid tumor malignancies.
flow cytometry, immuno-oncology
WebinarFlow cytometry in microbiological research 
In recent years the application of flow cytometry in microbiological research has expanded from detection and quantification of organisms to more complex studies including analysis of host-microbe interactions and detailed spatial and temporal analysis of microbial metabolism in different environments. During this webinar we will discuss how the multi-parametric nature of flow cytometry can be applied to microbiology and the advantages of using this application over traditional microbiological methods.
microbiology, multicolor flow cytometry, viability