Due to high background, cloning blunt-end and long PCR products can be difficult and often yields a low percentage of recombinants. Our unique Zero Background technology uses the lethal ccdB gene (control of cell death) gene to enable high-efficiency cloning.
The ccdB gene is incorporated into the cloning site of Zero Background vectors.The CcdB protein poisons bacterial gyrase, causing degradation of the hose chromosome and cell death. When an insert is ligated into the vector, the ccdB gene is disrupted, enabling only recombinant colonies to grow. By eliminating high vector background, the Zero Background technology yields nearly 100% recombinants, freeing you from having to screen hundreds of background colonies.
For Research Use Only. Not for use in diagnostic procedures.