QuantiGene Plex Data Analysis Software Icon

Streamlined gene expression analysis

Analyze data generated from QuantiGene Plex (QGP) assays with two freely available software options: QuantiGene Plex Data Analysis Software and Applied Biosystems Transcriptome Analysis Console (TAC) Software. These powerful software applications provide streamlined analysis and advanced visualization so you can get the most out of your data.

Data analysis workflow

Data analysis workflow for QuantiGene Plex Gene Expression Assays

QuantiGene Plex Data Analysis Software (Thermo Fisher Connect) introduces an improved workflow that provides customization capabilities for normalization and analysis of gene expression data generated using the QuantiGene Plex assay. Data from QuantiGene Plex Data Analysis Software can be exported into Transcriptome Analysis Console (TAC) Software for advanced analysis.
 

QuantiGene Plex Data Analysis Software

QuantiGene Plex Data Analysis Software is free software available through Connect, the Thermo Fisher Scientific cloud-based platform, that provides easy-to-follow workflows to improve data analysis of QuantiGene Plex assays. Data collected from QuantiGene Plex assays can be exported to Connect and conveniently accessed via web whenever an internet connection is available. Each Connect account offers 1 TB of cloud storage at no charge.

Access software

Software capabilities:

  • Control and treatment group assignments
  • Customize QC parameters
  • Analyze data from both 96-well and 384-well plates
  • Perform linearity of dilution calculations
  • Calculation of p-values during fold change analysis
  • Rank normalization genes for your analysis
  • View and export mean and individual sample values
  • Group samples and analyze the fold change in up to five groups
  • Remove samples or targets that do not meet QC criteria
  • Proceed directly to analysis without assigning background wells
Software workspaceFunctionFeatures

Setup plate map

Plate map set up

Set up plate for analysisAssign:
  • Samples to different groups for comparison
  • Background wells 
  • Wells that should not be included in the analysis

Normalization

Normalize data

Select and edit normalization genes and calculate assay linearity
  • Normalization ranking to calculate the stability measure (M) for each selected gene
  • Calculate assay linearity and % recovery

Review and edit QC criteria

Review and edit QC criteria

View and adjust the thresholds for QC parameters for data analysis
  • Maximum background
  • Limit of Detection
  • Technical Precision (%CV)
  • Saturation
  • Minimum Bead Count

Run analysis

  Run analysis

Run and analyze normalized results of differential gene expression analysisCalculate/view:
  • Change in expression of selected targets with respect to the reference sample or reference group and view graph

Create an account today
Already have an account? Access the QuantiGene Plex application.

Learn more about Thermo Fisher Cloud data security


Software highlights

Transcriptome Analysis Console

For enhanced data visualization and analysis beyond simple identification of differential expression, data can be exported from the QuantiGene Plex Data Analysis Software platform into Applied Biosystems Transcriptome Analysis Console (TAC) Software. Find tools to create scatter and volcano plots and perform hierarchical clustering. In addition, link out to publicly available annotations.

TAC Software capabilities:

  • Perform QC and data normalization
  • Perform statistical tests for differential expression
  • Focus on genes or pathways of interest
  • Explore interactions between coding and non-coding RNA
  • Link out to publicly available annotations
  • Obtain sequence information to design validation experiments

Download the latest version of TAC Software (64-bit). This version is supported on Microsoft Windows™ 7 (SP 1) and Windows 10 Professional.

Download  Release notes

Video: How to set up an Invitrogen™ QuantiGene™ Plex Assay in the Luminex® xPONENT® Software

Video: How to use the Invitrogen™ QuantiGene™ Plex Data Analysis App on the Thermo Fisher Connect Platform

QuantiGene Plex Data Analysis Software

QuantiGene Plex Data Analysis Software is free software available through Connect, the Thermo Fisher Scientific cloud-based platform, that provides easy-to-follow workflows to improve data analysis of QuantiGene Plex assays. Data collected from QuantiGene Plex assays can be exported to Connect and conveniently accessed via web whenever an internet connection is available. Each Connect account offers 1 TB of cloud storage at no charge.

Access software

Software capabilities:

  • Control and treatment group assignments
  • Customize QC parameters
  • Analyze data from both 96-well and 384-well plates
  • Perform linearity of dilution calculations
  • Calculation of p-values during fold change analysis
  • Rank normalization genes for your analysis
  • View and export mean and individual sample values
  • Group samples and analyze the fold change in up to five groups
  • Remove samples or targets that do not meet QC criteria
  • Proceed directly to analysis without assigning background wells
Software workspaceFunctionFeatures

Setup plate map

Plate map set up

Set up plate for analysisAssign:
  • Samples to different groups for comparison
  • Background wells 
  • Wells that should not be included in the analysis

Normalization

Normalize data

Select and edit normalization genes and calculate assay linearity
  • Normalization ranking to calculate the stability measure (M) for each selected gene
  • Calculate assay linearity and % recovery

Review and edit QC criteria

Review and edit QC criteria

View and adjust the thresholds for QC parameters for data analysis
  • Maximum background
  • Limit of Detection
  • Technical Precision (%CV)
  • Saturation
  • Minimum Bead Count

Run analysis

  Run analysis

Run and analyze normalized results of differential gene expression analysisCalculate/view:
  • Change in expression of selected targets with respect to the reference sample or reference group and view graph

Create an account today
Already have an account? Access the QuantiGene Plex application.

Learn more about Thermo Fisher Cloud data security


Software highlights

Transcriptome Analysis Console

For enhanced data visualization and analysis beyond simple identification of differential expression, data can be exported from the QuantiGene Plex Data Analysis Software platform into Applied Biosystems Transcriptome Analysis Console (TAC) Software. Find tools to create scatter and volcano plots and perform hierarchical clustering. In addition, link out to publicly available annotations.

TAC Software capabilities:

  • Perform QC and data normalization
  • Perform statistical tests for differential expression
  • Focus on genes or pathways of interest
  • Explore interactions between coding and non-coding RNA
  • Link out to publicly available annotations
  • Obtain sequence information to design validation experiments

Download the latest version of TAC Software (64-bit). This version is supported on Microsoft Windows™ 7 (SP 1) and Windows 10 Professional.

Download  Release notes

Video: How to set up an Invitrogen™ QuantiGene™ Plex Assay in the Luminex® xPONENT® Software

Video: How to use the Invitrogen™ QuantiGene™ Plex Data Analysis App on the Thermo Fisher Connect Platform


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