• Isolated from mouse embryos at day E13.5
  • Expanded and inactivated before cryopreservation at passage 3 (P3)
  • Exhibits more than 80% post-thaw viability
  • ESC/iPSCs cultured on Gibco MEFs retain normal morphology and pluripotent marker expression

Morphology of ESCs grown on GIBCO® MEFs (inactivated)



Figure 1. Bright-field images of ESCs.

(A) H9 human embryonic stem cells (hESCs) cultured on a layer of MEF feeder cells. The bright-field image of the hESCs at passage 1 was obtained with a 10x objective.
(B) C57 mouse embryonic stem cells (mESCs) cultured on a layer of MEF feeder cells. Bright-field images of the mESCs were taken 2 days after plating, with a 10x objective.

ESCs cultured on GIBCO ® MEFs retain pluripotent marker expression



Figure 2. Immunostaining of H9 (hESCs) cultured on MEF
s for Oct4 expression.
(A) Green color indicates Oct4 expression.
(B) Blue color is (nuclear staining) DAPI staining of the same field. Image taken at 10x.