Studies of LRRK2 (leucine-rich repeat kinase 2) function and inhibition have been hampered by a lack of tools to express full-length LRRK2 in diverse cell types as well as high-throughput biochemical and cell-based assays.

Now, LRRK2 researchers can access full-length protein, HTS assays and BacMam particles from Life Technologies that can be used to identify compounds that inhibit kinase activity in WT-LRRK2, the Parkinson’s disease-associated G2019S and other variants.

Poster: Express Full-Length LRRK2 and Profile Inhibitors using Cell-Based and Biochemical Assays

Available LRRK2 Reagents

SKULRRK2 ReagentsAmino  AcidsTagBiochemical Activity AssaysBiochemical
Binding
Assays
Cellular AssaysOrdering Information
PV4873LRRK2 protein970-2527GST

yes

yes

 Order
A13388BacMam LRRK21 - 2527   

yes

Order

A14170 BacMam LRRK2-GFP1 - 2527GFP

 

yes

yes

Order

A14171LRRK2-GFP Lysate1 - 2527GFP 

yes

 Order
A14685 Lanthascreen LRRK2 (pSer935) Cellular assay kit1 - 2527

GFP

  

yes

Order

A15197LRRK2 WT Full Length Protein (10 µg)1-2527DYKDDDDK

yes

  Order
A15198LRRK2 WT Full Length Protein (100 µg)1-2527DYKDDDDK

yes

  Order
A15199LRRK2 WT Full Length Protein (1 mg)1-2527DYKDDDDK

yes

  Order
SKULRRK2     ReagentsAmino    AcidsTagBiochemical Activity AssaysBiochemical
Binding
Assays
Cellular AssaysOrdering Information
PV4881LRRK2 G2019S970-2527GST

yes

yes

 Order
A13389BacMam LRRK2 G2019S1 - 2527   

yes

Order

A14174BacMam LRRK2-GFP G2019S1 - 2527GFP

 

 

yes

Order

A14172LRRK2-GFP G2019S Lysate1 - 2527GFP 

yes

 Order
A14686 Lanthascreen LRRK2 G2019S (pSer935) Cellular assay kit1 - 2527GFP 

yes

 

Order

A15200LRRK2 WT Full Length Protein (10 µg)1-2527DYKDDDDK

yes

 

 

Order
A15201LRRK2 WT Full Length Protein (100 µg)1-2527DYKDDDDK

yes

  Order
A15202LRRK2 WT Full Length Protein (1 mg)1-2527 DYKDDDDK

yes

  Order
SKULRRK2 ReagentsAmino AcidsTagBiochemical Activity AssaysBiochemical Binding AssaysCellular AssaysOrdering Information
PV6051LRRK2 D1994A protein970-2527GST

 

 

Kinase "Dead"  engineered Mutation (useful as a control)

 

 Order
A13390BacMam LRRK2 D1994A1 - 2527  

yes

Order

Please inquireBacMam LRRK2-GFP D1994A 1 - 2527GFP

 

yes

Inquire

SKULRRK2 ReagentsAmino  AcidsTagBiochemical Activity AssaysBiochemical
Binding
Assays
Cellular AssaysOrdering Information
PV4873LRRK2 protein970-2527GST

yes

yes

 Order
A13388BacMam LRRK21 - 2527   

yes

Order

A14170 BacMam LRRK2-GFP1 - 2527GFP

 

yes

yes

Order

A14171LRRK2-GFP Lysate1 - 2527GFP 

yes

 Order
A14685 Lanthascreen LRRK2 (pSer935) Cellular assay kit1 - 2527

GFP

  

yes

Order

A15197LRRK2 WT Full Length Protein (10 µg)1-2527DYKDDDDK

yes

  Order
A15198LRRK2 WT Full Length Protein (100 µg)1-2527DYKDDDDK

yes

  Order
A15199LRRK2 WT Full Length Protein (1 mg)1-2527DYKDDDDK

yes

  Order
SKULRRK2     ReagentsAmino    AcidsTagBiochemical Activity AssaysBiochemical
Binding
Assays
Cellular AssaysOrdering Information
PV4881LRRK2 G2019S970-2527GST

yes

yes

 Order
A13389BacMam LRRK2 G2019S1 - 2527   

yes

Order

A14174BacMam LRRK2-GFP G2019S1 - 2527GFP

 

 

yes

Order

A14172LRRK2-GFP G2019S Lysate1 - 2527GFP 

yes

 Order
A14686 Lanthascreen LRRK2 G2019S (pSer935) Cellular assay kit1 - 2527GFP 

yes

 

Order

A15200LRRK2 WT Full Length Protein (10 µg)1-2527DYKDDDDK

yes

 

 

Order
A15201LRRK2 WT Full Length Protein (100 µg)1-2527DYKDDDDK

yes

  Order
A15202LRRK2 WT Full Length Protein (1 mg)1-2527 DYKDDDDK

yes

  Order
SKULRRK2 ReagentsAmino AcidsTagBiochemical Activity AssaysBiochemical Binding AssaysCellular AssaysOrdering Information
PV6051LRRK2 D1994A protein970-2527GST

 

 

Kinase "Dead"  engineered Mutation (useful as a control)

 

 Order
A13390BacMam LRRK2 D1994A1 - 2527  

yes

Order

Please inquireBacMam LRRK2-GFP D1994A 1 - 2527GFP

 

yes

Inquire

yes  indicates reagents have been shown to work in assays. Biochemical assays include Adapta®, LanthaScreen® activity and Eu-kinase binding assays. Cellular assays include localization and activity assays measuring Ser935 phosphorylation. For specific assay functionality details, click the linked SKU numbers.

As part of our ongoing support of MJFF, consortium members may be eligible for special pricing.  Please contact your sales representative for details.

Enlarge Image
Figure 1. LRRK2 mutations and domains: The above ideogram depicts the full-length, 2527 amino acid, LRRK2 protein and its domains: ankyrin-like repeats (ANK), leucine-rich repeats (LRR), Ras of complex protein, GTPase domain (ROC), C-terminal of ROC domain (COR), kinase domain (kinase), and WD40 repeats domain (WD40).   Also, depicted are four variants available from Life Technologies: G2019S, the most common Parkinson’s disease (PD) segregating polymorphism, which is associated with increased kinase activity; I2020T also segregates with PD, but the functional effect is unknown; D1994A is an engineered variant that renders the kinase inactive; R1441C is a natural PD segregating variant, which is suspected to decrease GTPase activity. S935, shown in grey, indicates an internal phosphorylation site, against which specific antibodies have been generated. Truncated proteins span amino acids 970-2527, and do not contain the N-terminus, ANK domain, nor the internal S935 phosphorylation site. For more information regarding LRRK2 see Nature Reviews Neuroscience (2010), v11, pp791-797.

Additional LRRK2 Reagents

Custom Assays

Don’t see what you need?  Our custom biology team can build tools to fit your requirements. Contact us to learn more.

For Research Use Only. Not for use in diagnostic procedures.

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