Hydrazides & biocytins for neuron staining

These low molecular weight polar tracers cross gap junctions and follow neuronal projections. Offered in a range of fluorescent colors, they can be multiplexed with other probes, and biocytins easily accept secondary detection for effective signal amplification.


Select hydrazides & biocytins for neuron staining

Hydrazides & Biocytins for Neuron Staining

  • Load by microinjection or iontophoresis
  • Fixable and photostable
  • Able to cross gap junctions
  • Use direct detection or signal amplification

With low molecular weights (<1,000 Da) and bright fluorescent signals, these cell-impermeant polar tracers can be employed to trace neuronal projections and investigate gap junctions. They are fixable and photostable and are offered in multiple colors for multiplexing with other fluorescent probes. Biocytins can also be detected using fluorescent streptavidin conjugates for signal amplification.

Because they cross gap junctions, biocytins and hydrazides are often used to investigate neuronal communication in conjunction with labeled dextrans, which are retained within the cell.

  Biocytins and hydrazides
Confocal image stack of a 10,000 MW Calcium Green dextran–labeled climbing fiber in a sagittal cerebellar slice, showing incoming axon and terminal arborization (in yellow). The Purkinje cell innervated by this fiber was labeled with Alexa Fluor® 568 hydrazide.

Selection Guides for Hydrazides and Biocytins

Properties

Readout Direct fluorescence imaging of cells
Tissue sections Yes
In vivo neurons Yes
Fixed tissue sections No
Cultured cells Yes
Cell loading Microinjection, iontophoresis
Staining mode Endogenous
Best for Single cells
Fixable 5 stars
Transport Bidirectional
Photobleaching resistance 5 stars
Synaptic transfer No
Gap junction transfer Yes
Bibliography Citations

 

Selection Guide

 
Carrier
Standard filter set(s)
Fluorophore
Ex/Em (nm)
Quantity
Cat. No.
Alexa Fluor® 350 Hydrazide Hydrazide, sodium salt DAPI Alexa Fluor® 350 346/442 5 mg A10439
Alexa Fluor® 488 Hydrazide FITC Alexa Fluor® 488 495/519 5 mg A10436
Alexa Fluor® 568 Hydrazide Rhodamine Alexa Fluor® 568 578/603 5 mg A10437
Alexa Fluor® 594 Hydrazide Texas Red® Alexa Fluor® 594 590/617 5 mg A10438
Alexa Fluor® 555 Hydrazide Hydrazide, Tris(triethylammonium)
salt
TRITC Alexa Fluor® 555 555/565 1 mg A20501MP
Alexa Fluor® 633 Hydrazide Texas Red® Alexa Fluor® 633 632/647 1 mg A30634
Alexa Fluor® 647 Hydrazide Cy®5 Alexa Fluor® 647 650/668 1 mg A20502

Properties

Readout
Direct fluorescence imaging of cells; biocytins easily accept secondary detection to amplify weak signals
Tissue sections Yes
In vivo neurons Yes
Fixed tissue sections No
Cultured cells Yes
Cell loading Microinjection, iontophoresis
Staining mode Endogenous
Best for Single cells
Fixable 5 stars
Transport Bidirectional
Photobleaching resistance 5 stars
Synaptic transfer No
Gap junction transfer Yes
Bibliography Citations

 

Selection Guide

  Carrier
Standard filter set(s)
Fluorophore
Ex/Em (nm)
Quantity
Cat. No.
Alexa Fluor® 488 Biocytin

Biocytin,
sodium salt

FITC Alexa Fluor® 488 495/519 250 μg A12924
Alexa Fluor® 546 Biocytin TRITC Alexa Fluor® 546 556/573 250 μg A12923
Alexa Fluor® 594 Biocytin Texas Red® Alexa Fluor® 594 590/617 250 μg A12922