Dynabeads Intact Virus Enrichment (optimized for SARS-CoV-2)

For functional studies of SARS-CoV-2, such as growth rate and infectivity, live intact virus is required. Viruses can be present in very low concentrations in samples such as cell culture media and wastewater. In order to perform downstream analyses, it is often necessary to purify and concentrate the virus from the sample matrix.
 

Methods of intact virus purification

Coronaviruses such as SARS-CoV-2 can be easily purified and concentrated into a smaller volume by either precipitation followed by centrifugation, or by using magnetic beads. Each method of virus purification has specific benefits so your choice will depend on your needs.
 

Virus purification by precipitation

Addition of Invitrogen Virus Precipitation Reagent to a dilute sample results in less-soluble components (i.e., virus) being forced out of solution. The precipitated virus is collected after a brief centrifugation—no ultracentrifugation is required. This virus purification process is simple, well-suited to a large volume of starting material, and requires only about three hours with minimal hands-on time.
 

Virus purification by magnetic beads

Magnetic bead-based methods involve exposing the virus-containing sample to micron-sized magnetic beads possessing a strong anion exchange surface; after mixing for ten minutes, the virus-bound beads are collected via magnet, washed, and eluted in a small volume to yield concentrated virus. Virus purification by Dynabeads Intact Virus Enrichment magnetic beads is a rapid, convenient, and automatable way to concentrate virus-containing solutions.

Learn more about isolating SARS-CoV-2 from wastewater samples using Dynabeads magnetic beads

Virus concentration kits for SARS-CoV-2 research

Thermo Fisher Scientific offers two products optimized for SARS-CoV-2 virus purification.

Both methods of virus purification are suitable for live or inactivated SARS-CoV-2 virus, SARS-CoV-2 virus-like particles (VLPs), other enveloped viruses and vesicles present in cell culture media, virus transport media, and wastewater samples.

Purified SARS-CoV-2 virus can be used in:

  • Functional studies
  • Protein analysis (e.g., western blot)
  • Nucleic acid research (e.g., RT-qPCR)
     

Methods of intact virus purification

 Intact Virus Precipitation Reagent (optimized for SARS-CoV-2) for enriching virus samples

Intact Virus Precipitation Reagent (optimized for SARS-CoV-2)

Dynabeads Intact Virus Enrichment (optimized for SARS-CoV-2), appropriate for use in manual and automated magnet-based isolation procedures

Dynabeads Intact Virus Enrichment (optimized for SARS-CoV-2)

Benefits
  • Simple—Three-step SARS-CoV-2 concentration; requires just a centrifuge
  • Fast—Enrichment in less than three hours with very little hands-on time
  • Volume—Dilute virus samples can be enriched from large volumes
  • Straight to PCR—No further nucleic acid extraction is needed prior to PCR
  • Automated & simple—Just push the button and walk away
  • Very fast—Isolate intact SARS-CoV-2 virus in ~20 minutes
  • Easy release—The intact virus can be released from the beads in ~10 minutes
  • High-throughput—Enrich up to 96 samples per run
Best forLarge-volume samples
  • Automated enrichment of intact virus
  • Wastewater samples
  • Mass spectrometry analysis
No. of samples/run1–24 (Determined by centrifuge size/capacity)
  • 1–12 samples with KingFisher Duo Prime instrument
  • 1–24 samples (24-well plate) with KingFisher Flex instrument
  • 1–96 samples (96-well plate) with KingFisher Flex instrument
Total processing time~3 hours (or overnight incubation, if preferred)20–30 minutes
Automation compatibilityNoYes
Cat. No.10720D10700D

 

Virus purification videos

Virus purification using Invitrogen Intact Virus Precipitation Reagent

Virus purification using Invitrogen Dynabeads Intact Virus Enrichment

KingFisher virus concentration protocols optimized for SARS-CoV-2

Ordering information for virus purification products

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Customized Dynabeads for OEM supply

Do you need to commercialize an assay or service function based on any of these virus enrichment products? Are you looking to customize a Dynabeads product for your future commercial needs? Browse our custom bead offerings with Dynabeads Plus then tell us how we can help you!

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