Dialysis Products | Thermo Fisher Scientific

Thermo Scientific dialysis units help facilitate the rapid and trouble-free dialysis of sample volumes from 10 μL to 250 mL. Unlike standard flat tubing, these innovative devices do not require knots or clips that can lead to leaking and sample loss.

Pierce 96-well Microdialysis Plates and Slide-A-Lyzer Dialysis MINI Devices are ideal for small volumes, Slide-A-Lyzer G3 Dialysis Cassettes are recommended for small to medium volumes, and Slide-A-Lyzer Dialysis Flasks are recommended for larger volumes.

Excellent sample recoveries—low-binding plastic and membrane help minimize sample loss compared to filtration and resin systems
Convenient—easy-to-grip format helps simplify sample addition and removal with syringe and/or pipette
Secure—sealed membranes help prevent leakage that can occur with dialysis tubing and homemade devices
Validated—each device is leak-tested during production

Choose the right dialysis device for your experiments


	Pierce 96-well Microdialysis Plates	Slide-A-Lyzer MINI Dialysis Devices	Slide-A-Lyzer G3 Dialysis Cassettes	Slide-A-Lyzer Dialysis Flasks
External float required?	No	No	No	Optional
Method of sample addition/removal	Pipette	Pipette	Pipette	Pipette
Volume range	10–100 μL	10 μL–2 mL	3–125 mL	150–250 mL
Number of devices with different volume capacities	1	3	5	1
Molecular weight cutoffs (MWCOs)	3.5K, 10K	2K (0.1 mL size only), 3.5K, 10K, 20K	2K, 3.5K, 10K, 20K	2K, 3.5K, 10K, 20K
Automation compatible?	Yes	No	No	No
Integrated buffer reservoir?	Yes	Yes, except 0.1 mL size	No	No
Gamma-irradiated options?	No	No	No (E-Beam irradiated available)	No
Molecular weight cutoff (MWCO) color coded?	No	Yes	Yes	Yes
	Learn more	Learn more	Learn more	Learn more

Learn more about our dialysis products

2K MWCO

Sample retention by the 2K MWCO Thermo Scientific Slide-A-Lyzer Cassette membrane. Vitamin B12, bacitracin, tyrosine kinase peptide 1, biotin-TPKs substrate, protein kinase Ce (PKCe) peptide substrate, and insulin A chain model systems (0.5-1 mg/mL) in either saline or 0.2 M carbonate bicarbonate buffer, pH 9.4 were dialyzed overnight (17 hours) at 4°C. The amount of retentate was estimated using either the Thermo Scientific Pierce BCA Protein Assay or absorption at 360 nm (for vitamin B12).

3.5K MWCO

Sample retention by the 3.5K MWCO Thermo Scientific Slide-A-Lyzer Cassette membrane. Vitamin B12, insulin B chain, and insulin (1 mg/mL) in either saline or 0.2 M carbonate bicarbonate buffer, pH 9.4 were dialyzed overnight (17 hours) at 4°C. The amount of retentate was estimated using either the Pierce BCA Protein Assay or absorption at 360 nm (for vitamin B12).

7K MWCO

Sample retention by the 7K MWCO Thermo Scientific Slide-A-Lyzer Cassette membrane. Vitamin B12, insulin B chain, insulin, and cytochrome C (1 mg/mL) in either 0.15 M sodium chloride or 0.2 M carbonate bicarbonate buffer, pH 9.4 were dialyzed overnight (17 hours) at 4°C. The amount of retentate was estimated using either the Pierce BCA Protein Assay or absorption at 360 nm (for vitamin B12).

10K MWCO

Sample retention by the 10K MWCO Thermo Scientific Slide-A-Lyzer Cassette membrane. Insulin B chain, insulin, and cytochrome C (1 mg/mL) in either 0.15 M sodium chloride or 0.2 M carbonate bicarbonate buffer, pH 9.4 were dialyzed overnight (17 hours) at 4°C. The amount of retentate was estimated using the Pierce BCA Protein Assay.

20K MWCO

Sample retention by the 20K MWCO Thermo Scientific Slide-A-Lyzer Cassette membrane. Insulin B chain, cytochrome C, lysozyme, and myoglobin were dialyzed overnight (17 hours) at 4°C in PBS, pH 7.4. The amount of retentate was estimated using the Pierce BCA Protein Assay.

Thermo Scientific Slide-A-Lyzer Cassettes protect your precious samples during dialysis and help minimize the hassles and risks of losing your samples using slippery tubing. They're available in sizes from 10 µL to 250 mL and in a full range of MWCOs.


Traditional tubing	vs.	Cassette
Slippery when wet Flat tubing is difficult to handle when wet, increasing risk of sample loss during sample addition/removal	sample handling	Easy handling and secure sample delivery Plastic frame and cap allow for secure sample addition/removal
Risk of sample loss Sample can leak out if clamp becomes loose or falls off	sample recovery	Sample protection Welded membrane and leak-proof cap helps minimize the risk of sample loss
Time consuming Non-optimized design that typically requires overnight dialysis	speed	Fast and efficient Innovative design with high surface/volume ratio allows for more rapid dialysis than conventional tubing

Comparison of Thermo Scientific Slide-A-Lyzer Cassettes with traditional dialysis tubing.

Thermo Scientific Slide-A-Lyzer Video

Other featured dialysis products

Snakeskin Dialysis Tubing

A ready-to-use form of traditional dialysis membrane tubing for 10 to 100 mL samples that does not require presoaking or boiling prior to use. SnakeSkin Dialysis Tubing is composed of regenerated cellulose and is available in 3 molecular weight cutoffs and 3 internal diameters.

Resources

For Research Use Only. Not for use in diagnostic procedures.