Protein Isolation and Purification Information

Learn about methods and technologies for efficient cell lysis, protein extraction and fractionation, targeted inhibition of unwanted protease and phosphatase activity, and convenient devices and high performance resins for the purification and clean-up of proteins and antibodies for downstream applications.

  • Cell lysis
  • Protein extraction
  • Protease & phosphatase inhibitors
  • Desalting & Dialysis
  • Sample clean-up
  • Affinity purification
  • Resins, beads, supports
  • Ligand immobilization
  • Immunoprecipitation (IP)
  • Technical handbooks
  • Protein methods articles
  • Product application notes
  • Support center resources
  • Protocols
  • Product selection guides

Protein isolation and purification features

Methods article

Affinity chromatography or purification is a powerful method whereby a protein of interest is purified from other components in a crude cell lysate or other sample by virtue of its specific binding properties to an immobilized ligand. 

Methods article

What is immunoprecipitation? IP is the small-scale affinity purification of antigens using a specific antibody that is immobilized to a solid support such as magnetic particles or agarose resin.


Protein isolation and purification learning resources

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TypeTitle
HandbookProtein Research Handbook (2016)
Methods articleThe Pierce Protein Methods Library
Methods articleAn Overview of Cell Lysis and Protein Extraction
Methods articleTraditional Methods of Cell Lysis
Methods articleDetergents for Cell Lysis and Protein Extraction
Methods articleCell Lysis Solutions
Methods articleCell Fractionation and Organelle Isolation
Methods articleProtease and Phosphatase Inhibitors
Methods articleAn Overview of Affinity Purification
Methods articleCovalent Immobilization of Affinity Ligands
Methods articleGST-tagged Proteins–Production and Purification
Methods articleHis-tagged Proteins–Production and Purification
Methods articleAn Overview of the Immunoprecipitation (IP) Technique
Methods articleAn Overview of Buffer Exchange
Methods articleDialysis Methods for Protein Research
Methods articleDesalting and Gel Filtration Chromatography
Selection guideCell Lysis and Fractionation (Selection Guides)
Selection guideDialysis Products Selection Guide
Selection guideDesalting Products Selection Guide
Selection guideProtein Purification (Selection Guides)
Selection guideImmunoprecipitation (Selection Guide)
Selection guideProtein Purification and Analytics Interactive Decision Tool
Selection guideBioprocessing and Manufacturing Protein Analytics and Production Tool
Application noteProduct Application Notes
Application noteAn improved method for highly efficient isolation of primary mouse embryonic fibroblasts. Easily isolate and culture primary mouse embryonic fibroblasts (MEF).
Application noteOne-hour procedure to isolate primary cardiomyocytes from neonatal mouse and rat hearts. Easily isolate and culture highly viable, functional primary cardiomyocytes.
Application noteBenchmarking experiments with Zeba Spin Desalting Columns. Protein recovery performance comparisons of Thermo Scientific Zeba Spin Desalting Columns to other commercially available desalting resins and columns.
Application noteCalculate the number of immobilized proteins per bead of agarose affinity supports. A consideration of relative sizes and dimensions of agarose resin beads, antibodies, proteins, chemical modification groups, and affinity ligands.
Application noteImproved, all-in-one B-PER Reagent for bacterial protein extraction. B-PER Complete Reagent provides gentle, efficient, and cost effective protein extraction from E. coli and other bacterial cells.
Application noteAn efficient method for the isolation of highly functional primary neurons. Easily and reproducibly isolate and culture primary neurons.
Application noteEfficient mammalian membrane protein extraction. Fast and simple enrichment of integral membrane proteins and membrane-associated proteins.
Application notePerformance characterization of Pierce Glutathione Superflow Agarose. Purity, dynamic binding capacity, reusability and chemical compatibility properties of a glutathione resin for FPLC purification of GST tagged fusion proteins.
Application notePerformance characterization of HisPur Ni-NTA Superflow Agarose. Purity, dynamic binding capacity, reusability and chemical compatibility properties of a nickel-charged IMAC resin for FPLC purification of His-tagged proteins.
Application notePerformance characterization of HisPur Cobalt Superflow Agarose. Purity, dynamic binding capacity, reusability and chemical compatibility properties of a cobalt-charged IMAC resin.
Application noteSeparation characteristics of dialysis membranes. Molecular weight cut-off (MWCO) specifications and rates of buffer exchange with Slide-A-Lyzer Dialysis Devices and Snakeskin Dialysis Tubing.
Application noteChromatography cartridges for desalting and affinity purification. Data using the cartridge format for automated liquid chromatography (LC) systems.
Application notePhosphoprotein enrichment from cell and tissue samples. Isolate phosphorylated proteins with an easy-to-use spin format
Application noteEliminate endotoxins from protein and antibody samples. A highly specific affinity resin enables endotoxin removal from various protein sources, sizes and charges.
Application noteRemoving endotoxins using a spin-column format. Fast and effective removal of endotoxins from protein samples.
Application noteFractionate subcellular proteins from tissue samples. Obtain proteins from five cellular components using a tissue-optimized kit.
Application noteMethod to isolate functional synaptosomes. Enrich neuronal synaptic proteins while maintaining phosphoprotein integrity.
Application noteComplete protein protection from proteases and phosphatases. Protease, phosphatase and combination inhibitor tablets provide a convenient format to protect proteins from degradation and dephosphorylation.
Application noteOptimized protein extraction reagent for neuronal tissue and primary cells. Obtain high yields of functional neuronal protein from all neuronal cell compartments.
Application noteUsing antibody-binding proteins for antibody purification. Choose a format based on the specific application and scale.
Application noteNew dimensions for low-volume sample dialysis. Self-contained devices give you the solution for high-protein recovery.
Application notePerform IP and co-IP experiments with high yield and low background. New Protein A/G magnetic beads enable manual and automated applications.
ProtocolProtein Isolation Protocols (for Dynabeads Magnetic Beads)