Protein Assays and Analysis Information

Learn about methods and technologies for identifying and measuring proteins. Understand protein quantitation reagents, ELISA and other immunoassays, protein interaction and activity assays, enzyme reporter assays, protein microarrays, and immunoprecipitation and pull-down tools.

BCA & Bradford protein assays
Fluorescent protein assays
BSA standards & A280
Immunoprecipitation (IP)
Pull-down assays
ELISA
Immunoassays
Blocking buffers
Multiplex & Microarrays

Enzyme reporter assays
Activity assays
Technical handbooks
Protein methods articles
Product application notes
Support center resources
Protocols
Product selection guides

Protein assays and analysis features

Methods article

Overview of ELISA

A review of the enzyme-linked immunsorbant assay (ELISA), including formats (sandwich), detection methods (direct, indirect), plate coating techniques, antibodies and other probes, buffers, and kits.

Methods article

Overview of Immunoprecipitation (IP) Technique

What is immunoprecipitation? IP is the small-scale affinity purification of antigens using a specific antibody that is immobilized to a solid support such as magnetic particles or agarose resin.

Protein assays and analysis learning resources

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Type	Title
Handbook	Protein Research Handbook (2016)
Handbook	Biomarker Quantitation Assay Guide to protein and RNA
Methods article	The Pierce Protein Methods Library
Methods article	An Overview of Protein Assays
Methods article	Chemistry of Protein Assays
Methods article	Protein Assay Data Analysis
Methods article	Protein-Protein Variation of Protein Assays
Methods article	An Overview of Detection Probes
Methods article	Secondary Antibodies as Probes
Methods article	Enzyme Probes
Methods article	Avidin-Biotin Interaction
Methods article	Fluorescent Probes
Methods article	Luciferase Reporters
Methods article	An Overview of ELISA
Methods article	ELISA Development and Optimization
Methods article	Spike-and-Recovery and Linearity-of-Dilution Assessment for ELISA
Methods article	Factors Affecting Signal Generation in ELISA
Methods article	ELISA Troubleshooting Guide
Methods article	ELISA Data Analysis
Methods article	An Overview of Immunohistochemistry
Methods article	Immunohistochemistry vs Immunocytochemistry
Methods article	Fixation Strategies and Formulations
Methods article	Paraffin Removal and Antigen Retrieval
Methods article	Methods to Block Endogenous Detection
Methods article	Blocking Strategies for IHC
Methods article	IHC Immunodetection
Methods article	Avidin-Biotin Complex Method for IHC Detection
Methods article	IHC Troubleshooting Guide
Methods article	Immunofluorescence Method for IHC Detection
Methods article	IHC Counterstains
Methods article	An Overview of the Immunoprecipitation (IP) Technique
Methods article	An Overview of Protein-Protein Interaction Analysis
Methods article	Co-Immunoprecipitation (Co-IP)
Methods article	Pull-Down Assays
Methods article	Crosslinking Protein Interaction Analysis
Methods article	Label Transfer Protein Interaction Analysis
Methods article	Far-Western Blot Analysis
Methods article	An Overview of Protein-Nucleic Acid Interactions
Methods article	Gel Shift Assays (EMSA)
Methods article	Methods for Detecting Protein-DNA Interactions
Methods article	Methods for Detecting Protein-RNA Interactions
Methods article	Chromatin IP (ChIP Assays)
Selection guide	Protein Assays (Selection Guide)
Selection guide	Immunoprecipitation (Selection Guide)
Selection guide	Immunoassays (Selection Guide, including Luminex Multiplex Assays)
Selection guide	Find ELISA Kits by Target (Selection Guide)
Selection guide	ELISA Enzyme Substrates Selection Guide
Selection guide	Luciferase Assay Selection Guide
Selection guide	NovaBright Reporter Gene Assay Selection Guide
Selection guide	Protein and Enzyme Activity Assay (Selection Guide)
Application note	Product Application Notes
Application note	Luciferase assays and BioTek Synergy microplate readers. Validation and comparison of single-step flash and dual-spectral luciferase reporter gene assays using the Synergy line of microplate readers.
Application note	Analysis of androgen-dependent and -independent regulation of transcriptional activity. Monitor activity using a sensitive and optimized magnetic ChIP kit.
Application note	Versatile luciferases: new tools for reporter assays. Microplate luminometers equipped with reagent dispensers and optical filters provide optimal performance for luciferase assays with Cypridina, Gaussia, Renilla and firefly luciferase reporter genes.
Application note	Activation of the antioxidant response pathway by pesticide chemicals. Simultaneous measurement of the activation of ARE- and NF-κB-driven luciferase reporters by parkinsonian neurotoxins in neuronal cells.
Application note	Monitoring neuronal differentiation using multiplexed luciferase reporters. Characterization of early neuronal differentiation markers in precursor cell lines using multiplexed luciferase reporters and immunofluorescence imaging.
Application note	Luciferase assays in hard-to-transfect Jurkat cells. Obtain strong signals in cells with low transfection efficiency.
Application note	Improved immobilization and conjugation of glycoproteins. Efficient aldehyde-hydrazide covalent coupling using a catalyst and an optimized buffer.
Application note	Perform IP and co-IP experiments with high yield and low background. New Protein A/G magnetic beads enable manual and automated applications.
Application note	Serine hydrolase active-site probes for activity-based enzyme profiling. New fluorophosphonate probes selectively label and enrich active serine hydrolases.
Application note	Detection and localization of active GTPases in neuronal cell differentiation. GTPase pull-down kits enable powerful signal transduction studies.
Application note	Enhanced immunostaining means more signal and better sensitivity. Achieve better protein detection using a fraction of your valuable primary antibody.
Application note	GTPase enrichment using a new active-site probe. Introducing a desthiobiotin nucleotide probe for studying GTPases.
Application note	Measure activation of small GTPases via their specific downstream effectors. Pull-down kits enrich active GTPases from cell or tissue lysates.

Protein Biology Resource Library

Browse protein biology learning center content by collection (resource type) — protein methods articles, product application notes, selection guides, webinars, technical handbooks, etc.