Protein Assays and Analysis Information

Learn about methods and technologies for identifying and measuring proteins. Understand protein quantitation reagents, ELISA and other immunoassays, protein interaction and activity assays, enzyme reporter assays, protein microarrays, and immunoprecipitation and pull-down tools.

  • BCA & Bradford protein assays
  • Fluorescent protein assays
  • BSA standards & A280
  • Immunoprecipitation (IP)
  • Pull-down assays
  • ELISA
  • Immunoassays
  • Blocking buffers
  • Multiplex & Microarrays
  • Enzyme reporter assays
  • Activity assays 
  • Technical handbooks
  • Protein methods articles
  • Product application notes
  • Support center resources
  • Protocols
  • Product selection guides

Protein assays and analysis features

Methods article

A review of the enzyme-linked immunsorbant assay (ELISA), including formats (sandwich), detection methods (direct, indirect), plate coating techniques, antibodies and other probes, buffers, and kits.

Methods article

What is immunoprecipitation? IP is the small-scale affinity purification of antigens using a specific antibody that is immobilized to a solid support such as magnetic particles or agarose resin.


Protein assays and analysis learning resources

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TypeTitle
HandbookProtein Research Handbook (2016)
HandbookBiomarker Quantitation Assay Guide to protein and RNA
Methods articleThe Pierce Protein Methods Library
Methods articleAn Overview of Protein Assays
Methods articleChemistry of Protein Assays
Methods articleProtein Assay Data Analysis
Methods articleProtein-Protein Variation of Protein Assays
Methods articleAn Overview of Detection Probes
Methods articleSecondary Antibodies as Probes
Methods articleEnzyme Probes
Methods articleAvidin-Biotin Interaction
Methods articleFluorescent Probes
Methods articleLuciferase Reporters
Methods articleAn Overview of ELISA
Methods articleELISA Development and Optimization
Methods articleSpike-and-Recovery and Linearity-of-Dilution Assessment for ELISA
Methods articleFactors Affecting Signal Generation in ELISA
Methods articleELISA Troubleshooting Guide
Methods articleELISA Data Analysis
Methods articleAn Overview of Immunohistochemistry
Methods articleImmunohistochemistry vs Immunocytochemistry
Methods articleFixation Strategies and Formulations
Methods articleParaffin Removal and Antigen Retrieval
Methods articleMethods to Block Endogenous Detection
Methods articleBlocking Strategies for IHC
Methods articleIHC Immunodetection
Methods articleAvidin-Biotin Complex Method for IHC Detection
Methods articleIHC Troubleshooting Guide
Methods articleImmunofluorescence Method for IHC Detection
Methods articleIHC Counterstains
Methods articleAn Overview of the Immunoprecipitation (IP) Technique
Methods articleAn Overview of Protein-Protein Interaction Analysis
Methods articleCo-Immunoprecipitation (Co-IP)
Methods articlePull-Down Assays
Methods articleCrosslinking Protein Interaction Analysis
Methods articleLabel Transfer Protein Interaction Analysis
Methods articleFar-Western Blot Analysis
Methods articleAn Overview of Protein-Nucleic Acid Interactions
Methods articleGel Shift Assays (EMSA)
Methods articleMethods for Detecting Protein-DNA Interactions
Methods articleMethods for Detecting Protein-RNA Interactions
Methods articleChromatin IP (ChIP Assays)
Selection guideProtein Assays (Selection Guide)
Selection guideImmunoprecipitation (Selection Guide)
Selection guideImmunoassays (Selection Guide, including Luminex Multiplex Assays)
Selection guideFind ELISA Kits by Target (Selection Guide)
Selection guideELISA Enzyme Substrates Selection Guide
Selection guideLuciferase Assay Selection Guide
Selection guideNovaBright Reporter Gene Assay Selection Guide
Selection guideProtein and Enzyme Activity Assay (Selection Guide)
Application noteProduct Application Notes
Application noteLuciferase assays and BioTek Synergy microplate readers. Validation and comparison of single-step flash and dual-spectral luciferase reporter gene assays using the Synergy line of microplate readers.
Application noteAnalysis of androgen-dependent and -independent regulation of transcriptional activity. Monitor activity using a sensitive and optimized magnetic ChIP kit.
Application noteVersatile luciferases: new tools for reporter assays. Microplate luminometers equipped with reagent dispensers and optical filters provide optimal performance for luciferase assays with Cypridina, Gaussia, Renilla and firefly luciferase reporter genes.
Application noteActivation of the antioxidant response pathway by pesticide chemicals. Simultaneous measurement of the activation of ARE- and NF-κB-driven luciferase reporters by parkinsonian neurotoxins in neuronal cells.
Application noteMonitoring neuronal differentiation using multiplexed luciferase reporters. Characterization of early neuronal differentiation markers in precursor cell lines using multiplexed luciferase reporters and immunofluorescence imaging.
Application noteLuciferase assays in hard-to-transfect Jurkat cells. Obtain strong signals in cells with low transfection efficiency.
Application noteImproved immobilization and conjugation of glycoproteins. Efficient aldehyde-hydrazide covalent coupling using a catalyst and an optimized buffer.
Application notePerform IP and co-IP experiments with high yield and low background. New Protein A/G magnetic beads enable manual and automated applications.
Application noteSerine hydrolase active-site probes for activity-based enzyme profiling. New fluorophosphonate probes selectively label and enrich active serine hydrolases.
Application noteDetection and localization of active GTPases in neuronal cell differentiation. GTPase pull-down kits enable powerful signal transduction studies.
Application noteEnhanced immunostaining means more signal and better sensitivity. Achieve better protein detection using a fraction of your valuable primary antibody.
Application noteGTPase enrichment using a new active-site probe. Introducing a desthiobiotin nucleotide probe for studying GTPases.
Application noteMeasure activation of small GTPases via their specific downstream effectors. Pull-down kits enrich active GTPases from cell or tissue lysates.