Protein Assays and Analysis Information

Learn about methods and technologies for identifying and measuring proteins. Understand protein quantitation reagents, ELISA and other immunoassays, protein interaction and activity assays, enzyme reporter assays, protein microarrays, and immunoprecipitation and pull-down tools.

  • BCA & Bradford protein assays
  • Fluorescent protein assays
  • BSA standards & A280
  • Immunoprecipitation (IP)
  • Pull-down assays
  • ELISA
  • Immunoassays
  • Blocking buffers
  • Multiplex & Microarrays
  • Enzyme reporter assays
  • Activity assays 
  • Technical handbooks
  • Protein methods articles
  • Product application notes
  • Support center resources
  • Protocols
  • Product selection guides

Protein assays and analysis features

Methods article

A review of the enzyme-linked immunsorbant assay (ELISA), including formats (sandwich), detection methods (direct, indirect), plate coating techniques, antibodies and other probes, buffers, and kits.

Methods article

What is immunoprecipitation? IP is the small-scale affinity purification of antigens using a specific antibody that is immobilized to a solid support such as magnetic particles or agarose resin.

Protein assays and analysis learning resources

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Type Title
Handbook Protein Research Handbook (2016)
Handbook Biomarker Quantitation Assay Guide to protein and RNA
Methods article The Pierce Protein Methods Library
Methods article An Overview of Protein Assays
Methods article Chemistry of Protein Assays
Methods article Protein Assay Data Analysis
Methods article Protein-Protein Variation of Protein Assays
Methods article An Overview of Detection Probes
Methods article Secondary Antibodies as Probes
Methods article Enzyme Probes
Methods article Avidin-Biotin Interaction
Methods article Fluorescent Probes
Methods article Luciferase Reporters
Methods article An Overview of ELISA
Methods article ELISA Development and Optimization
Methods article Spike-and-Recovery and Linearity-of-Dilution Assessment for ELISA
Methods article Factors Affecting Signal Generation in ELISA
Methods article ELISA Troubleshooting Guide
Methods article ELISA Data Analysis
Methods article An Overview of Immunohistochemistry
Methods article Immunohistochemistry vs Immunocytochemistry
Methods article Fixation Strategies and Formulations
Methods article Paraffin Removal and Antigen Retrieval
Methods article Methods to Block Endogenous Detection
Methods article Blocking Strategies for IHC
Methods article IHC Immunodetection
Methods article Avidin-Biotin Complex Method for IHC Detection
Methods article IHC Troubleshooting Guide
Methods article Immunofluorescence Method for IHC Detection
Methods article IHC Counterstains
Methods article An Overview of the Immunoprecipitation (IP) Technique
Methods article An Overview of Protein-Protein Interaction Analysis
Methods article Co-Immunoprecipitation (Co-IP)
Methods article Pull-Down Assays
Methods article Crosslinking Protein Interaction Analysis
Methods article Label Transfer Protein Interaction Analysis
Methods article Far-Western Blot Analysis
Methods article An Overview of Protein-Nucleic Acid Interactions
Methods article Gel Shift Assays (EMSA)
Methods article Methods for Detecting Protein-DNA Interactions
Methods article Methods for Detecting Protein-RNA Interactions
Methods article Chromatin IP (ChIP Assays)
Selection guide Protein Assays (Selection Guide)
Selection guide Immunoprecipitation (Selection Guide)
Selection guide Immunoassays (Selection Guide, including Luminex Multiplex Assays)
Selection guide Find ELISA Kits by Target (Selection Guide)
Selection guide ELISA Enzyme Substrates Selection Guide
Selection guide Luciferase Assay Selection Guide
Selection guide NovaBright Reporter Gene Assay Selection Guide
Selection guide Protein and Enzyme Activity Assay (Selection Guide)
Application note Product Application Notes
Application note Luciferase assays and BioTek Synergy microplate readers. Validation and comparison of single-step flash and dual-spectral luciferase reporter gene assays using the Synergy line of microplate readers.
Application note Analysis of androgen-dependent and -independent regulation of transcriptional activity. Monitor activity using a sensitive and optimized magnetic ChIP kit.
Application note Versatile luciferases: new tools for reporter assays. Microplate luminometers equipped with reagent dispensers and optical filters provide optimal performance for luciferase assays with Cypridina, Gaussia, Renilla and firefly luciferase reporter genes.
Application note Activation of the antioxidant response pathway by pesticide chemicals. Simultaneous measurement of the activation of ARE- and NF-κB-driven luciferase reporters by parkinsonian neurotoxins in neuronal cells.
Application note Monitoring neuronal differentiation using multiplexed luciferase reporters. Characterization of early neuronal differentiation markers in precursor cell lines using multiplexed luciferase reporters and immunofluorescence imaging.
Application note Luciferase assays in hard-to-transfect Jurkat cells. Obtain strong signals in cells with low transfection efficiency.
Application note Improved immobilization and conjugation of glycoproteins. Efficient aldehyde-hydrazide covalent coupling using a catalyst and an optimized buffer.
Application note Perform IP and co-IP experiments with high yield and low background. New Protein A/G magnetic beads enable manual and automated applications.
Application note Serine hydrolase active-site probes for activity-based enzyme profiling. New fluorophosphonate probes selectively label and enrich active serine hydrolases.
Application note Detection and localization of active GTPases in neuronal cell differentiation. GTPase pull-down kits enable powerful signal transduction studies.
Application note Enhanced immunostaining means more signal and better sensitivity. Achieve better protein detection using a fraction of your valuable primary antibody.
Application note GTPase enrichment using a new active-site probe. Introducing a desthiobiotin nucleotide probe for studying GTPases.
Application note Measure activation of small GTPases via their specific downstream effectors. Pull-down kits enrich active GTPases from cell or tissue lysates.

Protein Biology Resource Library

Browse protein biology learning center content by collection (resource type) — protein methods articles, product application notes, selection guides, webinars, technical handbooks, etc.

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For Research Use Only. Not for use in diagnostic procedures.