Removal of unreacted or residual small molecules in the protein sample preparation workflow is a key success factor for downstream analysis. For example, removal of free dye after a labeling reaction is essential for the accurate determination of dye-to-protein ratios and to help eliminate background noise. The Zeba Dye and Biotin Removal Spin Columns and 96-Well Filter Plates contain a ready-to-use resin that is uniquely designed for rapid removal of non-conjugated fluorescent dyes, biotin, unused reducing agents, and crosslinkers with exceptional protein recovery.
|Technology / Product||% removal (small molecule)||% recovery (protein)||No instrument required||Versatility||Sample dilution||Ease of use|
|Zeba Dye and Biotin Removal Columns and Plates|
|Size exclusion (column chromatography)|
|Size exclusion (spin) - BioRad® P30|
Thermo Scientific Zeba Dye and Biotin Removal Columns provide higher biotin removal with excellent protein recovery compared to alternative products.
Clean up your protein sample by removing unwanted dye, biotin, and crosslinkers. This tool can help you get better immunofluorescence images.
Thermo Scientific Dye and Biotin Removal Columns provide higher dye removal with excellent protein recovery compared to dialysis.
Figure 3 shows how you can achieve exceptional post-reaction protein sample cleanup result in as litle as 15 minutes.
Thermo Scientific Zeba Dye and Biotin Removal Columns provide efficient recovery of proteins of greater than 7 kDa.
Excellent dye removal and protein recovery can be achieved by using the Zeba Dye and Biotin Removal Columns.
Table 2. Protein recovery was assessed by applying 1 mg/mL samples of each protein to 5 mL columns. Protein concentrations of processed samples and starting material were quantified using Pierce Rapid Gold BCA Protein Assay Kit.
|% Recovery using 5 mL columns|
|Protein||MW (kDa)||2 mL sample|
Reducing agent removal from protein sample can be done easily and efficiently. Figure 4 compares the efficiency of removing TCEP with Scientific Zeba Dye and Biotin Removal Columns relative to comparable products from other suppliers.
Thermo Scientific Zeba Dye and Biotin Removal Columns provide higher dye removal with excellent protein recovery compared to products from other suppliers.
Figure 5 demonstrates how these columns enable a better and simpler way to remove dye from protein samples.
For Research Use Only. Not for use in diagnostic procedures.