Pierce Dye and Biotin Removal Spin Columns and 96-Well Filter Plates

Removal of unreacted or residual small molecules in the protein sample preparation workflow is a key success factor for downstream analysis. For example, removal of free dye after a labeling reaction is essential for the accurate determination of dye-to-protein ratios and to help eliminate background noise. The Zeba Dye and Biotin Removal Spin Columns and 96-Well Filter Plates contain a ready-to-use resin that is uniquely designed for rapid removal of non-conjugated fluorescent dyes, biotin, unused reducing agents, and crosslinkers with exceptional protein recovery.

Product highlights

  • Versatile—removes unreacted fluorescent dyes, biotinylation reagents, crosslinkers, and reducing agents from protein solutions
  • High recovery—low-binding resin maximizes protein recovery
  • Easy to use—no cumbersome column preparation or equilibration
  • Fast—small-molecule removal and protein recovery in less than 15 mins
  • Flexible—available in spin columns and filter spin plates for a range of needs

Applications

  • Ideal for post-reaction clean-up of samples
  • To effectively remove non-conjugated dye, biotin, reducing agents, and crosslinkers with exceptional protein recovery

Zeba Dye and Biotin Removal Columns and Plates

Format 0.5 mL spin columns 2 mL spin columns 5 mL spin columns 10 mL spin columns Filter plates, 96 well
Resin bed volume 0.5 mL 2 mL 5 mL 10 mL 420uL
Volume capacity 50–120 μL 400–700 μL 1–2 mL 2–4 mL 40–100 uL
Molecular weight cutoffs (MWCOs) 7 kDa 7 kDa 7 kDa 7 kDa 7 kDa
Automation compatible? No No No No Yes

Product comparison

Technology / Product % removal (small molecule) % recovery (protein) Time saving No instrument required Versatility Sample dilution Ease of use
Zeba Dye and Biotin Removal Columns and Plates
Size exclusion (column chromatography)
Size exclusion (spin) - BioRad® P30
Ion exchange (Pierce Dye Removal Columns)
Dialysis (Slide-a-Lyzer Cassettes)

Better small molecule removal and protein recovery performance

Thermo Scientific Zeba Dye and Biotin Removal Columns provide higher biotin removal with excellent protein recovery compared to alternative products.

Better immunofluorescence images

Clean up your protein sample by removing unwanted dye, biotin, and crosslinkers. This tool will help you get better immunofluorescence images.

Better performance and time savings

Thermo Scientific Dye and Biotin Removal Columns provide higher dye removal with excellent protein recovery compared to dialysis.

Figure 3 shows how you can achieve superior post-reaction protein sample cleanup result in 15 minutes.

Excellent protein recovery

Thermo Scientific Zeba Dye and Biotin Removal Columns provide efficient recovery of proteins of greater than 7 kDa.

Excellent dye removal and protein recovery can be achieved by using the Zeba Dye and Biotin Removal Columns.

Table 2. Protein recovery was assessed by applying 1 mg/mL samples of each protein to 5 mL columns. Protein concentrations of processed samples and starting material were quantified using Pierce Rapid Gold BCA Protein Assay Kit.

  % Recovery using 5 mL columns
Protein MW (kDa) 2 mL sample
Insulin 5.8 29%
Cytochrome C 12 91%
Chymotrypsin 25.6 97%
Protein A 34 97%
Transferrin 80 94%
Rabbit IgG 150 89%

Higher reducing agent removal efficiency

Reducing agent removal from protein sample can be done easily and efficiently. Figure 4 compares the efficiency of removing TCEP with Scientific Zeba Dye and Biotin Removal Columns relative to comparable products from other suppliers.

Better dye removal performance

Thermo Scientific Zeba Dye and Biotin Removal Columns provide higher dye removal with excellent protein recovery compared to products from other suppliers.

Figure 5 demonstrates how these columns enable a better and simpler way to remove dye from protein samples.