Removal of unreacted or residual small molecules in the protein sample preparation workflow is a key success factor for downstream analysis. For example, removal of free dye after a labeling reaction is essential for the accurate determination of dye-to-protein ratios and to help eliminate background noise. The Pierce Dye and Biotin Removal Spin Columns and 96-Well Filter Plates contain a ready-to-use resin that is uniquely designed for rapid removal of non-conjugated fluorescent dyes, biotin, unused reducing agents, and crosslinkers with exceptional protein recovery.
- Versatile—removes unreacted fluorescent dyes, biotinylation reagents, crosslinkers, and reducing agents from protein solutions
- High recovery—low-binding resin maximizes protein recovery
- Easy to use—no cumbersome column preparation or equilibration
- Fast—small-molecule removal and protein recovery in less than 15 mins
- Flexible—available in spin columns and filter spin plates for a range of needs
- Ideal for post-reaction clean-up of samples
- To effectively remove non-conjugated dye, biotin, reducing agents, and crosslinkers with exceptional protein recovery
Choose the right biotin and dye removal product for your experiment
|Format||0.5 mL spin columns||2 mL spin columns||5 mL spin columns||10 mL spin columns||Filter plates, 96 well|
|Resin bed volume||0.5 mL||2 mL||5 mL||10 mL||420uL|
|Volume capacity||50–120 μL||400–700 μL||1-2 mL||2–4 mL||40 – 100 uL|
|Molecular weight cutoffs (MWCOs)||7KDa||7KDa||7KDa||7KDa||7KDa|
Table 1. Compare available product options for small molecule removal and protein recovery
|Technology / Product||% removal (small molecule)||% recovery (protein)||Time saving||No instrument required||Versatility||Sample dilution||Ease of use|
|Pierce dye and biotin Removal columns and plates|
|Size exclusion (column chromatography)|
|Size exclusion (spin) - BioRad® P30|
|Ion exchange (Pierce Dye Removal Columns)|
|Dialysis (Slide-a-Lyzer Cassettes)|
Better small molecule removal and protein recovery performance
Thermo Scientific Pierce Dye and Biotin Removal Columns provide higher biotin removal with excellent protein recovery compared to alternative products.
Better immunofluorescence images
Clean up your protein sample by removing unwanted dye, biotin, and crosslinkers. This tool will help you get better immunofluorescence images.
Better performance and time savings
Thermo Scientific Dye and Biotin Removal Columns provide higher dye removal with excellent protein recovery compared to dialysis.
Figure 3 shows how you can achieve superior post-reaction protein sample cleanup result in 15 minutes.
Excellent protein recovery
Thermo Scientific Pierce Dye and Biotin Removal Columns (Cat. No. A44298) provide efficient recovery of proteins of greater than 7 kDa.
Excellent dye removal and protein recovery can be achieved by using the Pierce Dye and Biotin Removal Columns.
Table 2. Protein recovery was assessed by applying 1 mg/mL samples of each protein to 5 mL columns (Cat. No. A44300). Protein concentrations of processed samples and starting material were quantified using Pierce Rapid Gold BCA Protein Assay Kit (Cat. No. A53226).
|% Recovery using 5 mL columns|
|Protein||MW (kDa)||2 mL sample|
Higher reducing agent removal efficiency
Reducing agent removal from protein sample can be done easily and efficiently. Figure 4 compares the efficiency of removing TCEP with Scientific Pierce Dye and Biotin Removal Columns relative to comparable products from other suppliers.
Better dye removal performance
Thermo Scientific Pierce Dye and Biotin Removal Columns provide higher dye removal with excellent protein recovery compared to products from other suppliers.
Figure 5 demonstrates how these columns enable a better and simpler way to remove dye from protein samples.
For Research Use Only. Not for use in diagnostic procedures.