Pierce Dye and Biotin Removal Spin Columns and 96-Well Filter Plates

Removal of unreacted or residual small molecules in the protein sample preparation workflow is a key success factor for downstream analysis. For example, removal of free dye after a labeling reaction is essential for the accurate determination of dye-to-protein ratios and to help eliminate background noise. The Zeba Dye and Biotin Removal Spin Columns and 96-Well Filter Plates contain a ready-to-use resin that is uniquely designed for rapid removal of non-conjugated fluorescent dyes, biotin, unused reducing agents, and crosslinkers with exceptional protein recovery.

Product highlights

  • Versatile—removes unreacted fluorescent dyes, biotinylation reagents, crosslinkers, and reducing agents from protein solutions
  • High recovery—low-binding resin maximizes protein recovery
  • Easy to use—no cumbersome column preparation or equilibration
  • Fast—small-molecule removal and protein recovery in less than 15 mins
  • Flexible—available in spin columns and filter spin plates for a range of needs

Applications

  • Ideal for post-reaction clean-up of samples
  • To effectively remove non-conjugated dye, biotin, reducing agents, and crosslinkers with exceptional protein recovery

Zeba Dye and Biotin Removal Columns and Plates

Format0.5 mL spin columns2 mL spin columns5 mL spin columns10 mL spin columnsFilter plates, 96 well
Resin bed volume0.5 mL2 mL5 mL10 mL420uL
Volume capacity50–120 μL400–700 μL1–2 mL2–4 mL40–100 uL
Molecular weight cutoffs (MWCOs)7 kDa7 kDa7 kDa7 kDa7 kDa
Automation compatible?NoNoNoNoYes

Product comparison

Technology / Product% removal (small molecule)% recovery (protein)No instrument requiredVersatilitySample dilutionEase of use
Zeba Dye and Biotin Removal Columns and Plates
Size exclusion (column chromatography)
Size exclusion (spin) - BioRad® P30

Better small molecule removal and protein recovery performance

Thermo Scientific Zeba Dye and Biotin Removal Columns provide higher biotin removal with excellent protein recovery compared to alternative products.

Better immunofluorescence images

Clean up your protein sample by removing unwanted dye, biotin, and crosslinkers. This tool will help you get better immunofluorescence images.

Better performance and time savings

Thermo Scientific Dye and Biotin Removal Columns provide higher dye removal with excellent protein recovery compared to dialysis.

Figure 3 shows how you can achieve superior post-reaction protein sample cleanup result in 15 minutes.

Excellent protein recovery

Thermo Scientific Zeba Dye and Biotin Removal Columns provide efficient recovery of proteins of greater than 7 kDa.

Excellent dye removal and protein recovery can be achieved by using the Zeba Dye and Biotin Removal Columns.

Table 2. Protein recovery was assessed by applying 1 mg/mL samples of each protein to 5 mL columns. Protein concentrations of processed samples and starting material were quantified using Pierce Rapid Gold BCA Protein Assay Kit.

 % Recovery using 5 mL columns
ProteinMW (kDa)2 mL sample
Insulin5.829%
Cytochrome C1291%
Chymotrypsin25.697%
Protein A3497%
Transferrin8094%
Rabbit IgG15089%

Higher reducing agent removal efficiency

Reducing agent removal from protein sample can be done easily and efficiently. Figure 4 compares the efficiency of removing TCEP with Scientific Zeba Dye and Biotin Removal Columns relative to comparable products from other suppliers.

Better dye removal performance

Thermo Scientific Zeba Dye and Biotin Removal Columns provide higher dye removal with excellent protein recovery compared to products from other suppliers.

Figure 5 demonstrates how these columns enable a better and simpler way to remove dye from protein samples.

For Research Use Only. Not for use in diagnostic procedures.