pMIR-REPORT miRNA Expression Reporter Vector
- Test sequences for putative miRNA binding sites
- Test interaction between miRNAs and known miRNA binding sites
- Monitor miRNA levels with sensitive, quantitative luciferase activity
- Control for transfection efficiency with ß-galactosidase activity
The pMIR-REPORT miRNA Expression Reporter Vector can be used as a screening tool in which random or non-random sequences are inserted into the vector to identify miRNA targets. When coupled with Pre-miR™ miRNA Precursor Molecules, pMIR-REPORT vectors can be used to identify miRNAs that bind to a target sequence of interest. As part of a complete solution, a second vector expressing ß-galactosidase is provided as a control to normalize transfection efficiency (Figure 1B).
pMIR-REPORT miRNA Expression Reporter Vector is the only vector available that has been extensively validated for use as a standard reporter assay for miRNA research. With the pMIR-REPORT vector, you'll be able to quantitate miRNA functional activity.
Figure 1. (A) pMIR-REPORT™ miRNA Expression Reporter Vector. (B) pMIR-REPORT™ ß-Galactosidase Control Vector.