Most of the product literature associated with our amine-reactive dyes provides protocols for labeling proteins, typically IgG antibodies in aqueous buffers (Amine-Reactive Probe Labeling Protocol). The following protocol is a starting point for labeling peptides in organic solvents. Please note that the reaction conditions, including concentrations of the reactants and the reaction times, may require optimization. Furthermore, many peptides are not soluble in a 100% organic solution. It is very important to test the solubility of the peptide in dimethylsulfoxide (DMSO) or dimethylformamide (DMF) before attempting this procedure.
For Research Use Only. Not for use in diagnostic procedures.