RNA Analysis with Low Copy Number Templates
Together, Ambion and Applied Biosystems (AB) provide researchers with a complete family of products that takes experiments from sample preparation through target detection and analysis.
For limited samples and small sample sizes, RNA can be extracted from numerous small tissue samples with one of several nucleic acid isolation kits from Ambion (see table). Quantitative analysis of gene expression can then be assessed using RT-PCR reagents and instruments from Applied Biosystems (Figure 1).
MELT™ Total Nucleic Acid Isolation System (patent pending) provides hands-free tissue digestion that takes only minutes—no grinding or use of a Polytron® homogenizer required—making MELT ideal for processing samples for downstream high throughput amplification.
MagMAX™-96 Total RNA Isolation System is optimized for high throughput isolation of RNA from 25 cells to 2 x 10 6 cells, as well as small plant and mammalian tissue samples (up to 10 mg). This magnetic bead-based technology binds RNA more efficiently than glass fiber filter methods, resulting in higher RNA yields.
RNAqueous®-Micro Kit is optimized for the purification of total RNA from micro-sized samples such as 10 to 5 x 10 5 cultured cells, as few as 10 microdissected cells, or up to 10 mg tissue. Samples are solubilized in a powerful chaotropic solution, and RNA is purified on a glass fiber filter cartridge specifically designed for very small samples. A simple modification of the protocol allows recovery of both large and small RNA species including tRNAs, 5S rRNA, and microRNAs.
RNAqueous®-4PCR Kit is designed to isolate RNA free of genomic DNA contamination from very small samples such as needle biopsies and small numbers of isolated cells (as little as 1 mg of tissue or 100 cells).
- Short-term and long-term archival storage
- Quantitative PCR
- Conversion to cRNA
(This figure is referenced in the sidebar text.)