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Dialysis Cassettes, Flasks, & Devices |
Dialysis cassettes are a secure alternative to traditional dialysis tubing and designed to be convenient. The Slide-A-Lyzer G3 dialysis cassette offers a rigid-framed, free-standing, and self-floating vessel with a secure cap closure and large surface area consisting of regenerated cellulose membranes for simple and efficient dialysis experiments. Samples can be loaded and retrieved easily using a serological pipette.
In contrast, traditional dialysis membrane tubing presents a number of challenges: the tubing bag is slippery when wet and requires knots or plastic clips to secure the sample. Leakage or contamination of the sample is likely because the membrane must be closed manually (see comparison table).
Dialysis cassette and flask selection guide
| Mini Dialysis Device (Dialysis Cups) | G3 Dialysis Cassettes | Dialysis Flasks | |
|---|---|---|---|
| Volume range | 10 μL–2 mL | 1–125 mL | 150–250 mL |
| External float required | Yes (0.1 mL size only) | No | Yes |
| Sample removal | Pipette | Pipette | Pipette, directly pouring |
| Molecular weight cutoffs (MWCOs) | 2K (0.1 mL size only), 3.5K, 10K, 20K | 2K, 3.5K, 10K, 20K | 2K, 3.5K, 10K, 20K |
Choose your Slide-A-Lyzer device by volume and MWCO
Click on the tabs below to explore dialysis devices, including cups, cassettes, and flasks.
Dialysis devices
Slide-A-Lyzer MINI dialysis devices have a cup-like design that is available in 3 volume sizes: 0.1 mL devices, which can be placed into a foam float during dialysis, and 0.5 mL and 2 mL sizes, which are capped by inserting them into the included 15 mL and 50 mL conical tubes, respectively. The tubes serve as dialysis reservoirs for easy and self-contained dialysis and are available in 2K, 3.5K, 7K, 10K, and 20K MWCOs.
Use the Slide-A-Lyzer MINI Dialysis Devices in applications including low molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, microconjugation reactions, and sample concentration.
Additional features of Slide-A-Lyzer MINI dialysis devices:
- High sample recovery—Low-binding membranes help ensure that proteins and other macromolecules are recovered.
- Ease-of-use—Disposable; no syringe adapters or other assembled equipment required.
- Chemical compatibility—Cellulose membranes are compatible with common chemicals and buffers.
Choose your MINI dialysis device based on volume and MWCO
The Slide-A-Lyzer MINI device makes dialyzing small volumes easy. Just add the sample, place the device in the conical tube (for 0.5 mL and 2 mL devices), recap, and shake for up to 4 hours or overnight. Use a pipet to recover the sample (Figure 1).
Figure 1. Slide-A-Lyzer MINI dialysis device workflow.
Table 1. Protein recovery percentage using the 2 mL MINI Dialysis Device.
| Membrane MWCO | Protein/Peptide | Recovery (%) |
|---|---|---|
| 3.5K | Insulin Chain B (3.5 kDa) | 90.13 |
| 10K | Cytochrome C (12.4 kDa) | 94.44 |
| 20K | Myoglobin (17 kDa) | 95 |
Insulin chain B, cytochrome C and myoglobin (0.25 mg/mL) in either 50 mM sodium phosphate, 75 mM NaCl at pH 7.2 or 0.2 M carbonate-bicarbonate buffer at pH 9.4 were dialyzed overnight (17 hours) at 4°C. The amount of protein in the retentate was determined using the Pierce BCA Protein Assay.
Slide-A-Lyzer MINI dialysis devices video
View the step-by-step instructional video that shows how to use the MINI dialysis devices.
Dialysis cassettes
Slide-A-Lyzer G3 dialysis cassettes accommodate volumes ranging from 1–125 mL, and are available in 2K, 3.5K, 10K, and 20K molecular weight cutoffs (MWCOs).
Use Slide-A-Lyzer G3 dialysis cassettes in various applications, including low molecular-weight contaminant and salt removal, buffer exchange, and sample concentration.
Additional features of the Slide-A-Lyzer G3 dialysis cassettes:
- Sample security—Sealed membranes prevent leakage that can occur with dialysis tubing.
- Ergonomic—A lighter and more compact frame enables easier handling, loading, and retrieval.
- Greener by design—The Slide-A-Lyzer G3 features up to a 68% reduction in plastic waste compared to previous generations while offering the same secure, high-performance dialysis. Refer to the Slide-A-Lyzer G3 green fact sheet for more information.
- Self-flotation—No external flotation device or clips are required.
Choose your G3 dialysis cassette based on volume and MWCO
The Slide-A-Lyzer G3 dialysis cassette is simple to use. Load the cassette with sample, put it into the dialysis buffer, and then retrieve the sample (Figure 2).
Figure 2. Slide-A-Lyzer G3 dialysis cassettes workflow.
Comparison of dialysis cassettes with traditional dialysis tubing
 |  | |
|---|---|---|
| Dialysis product | Traditional tubing | Cassette |
| Sample handling | Slippery when wet Flat tubing is difficult to handle when wet, increasing risk of sample loss during sample addition/removal | Easy handling and secure sample delivery Plastic frame and cap allow for secure sample addition/removal |
| Sample recovery | Risk of sample loss Sample can leak out if clamp becomes loose or falls off | Sample protection Welded membrane and leak-proof cap helps reduce the risk of sample loss |
| Speed | Time consuming Non-optimized design that typically requires overnight dialysis | Fast and efficient Innovative design with high surface/volume ratio allows for more rapid dialysis than conventional tubing |
Slide-A-Lyzer G3 dialysis cassettes video
View the step-by-step instructional video that shows how to use the G3 dialysis cassettes.
Dialysis flasks
Slide-A-Lyzer dialysis flasks facilitate simple and effective dialysis in larger sample volumes ranging from 150 mL up to 250 mL. These devices accomplish typical buffer exchange in 8–24 hours and are available in 2K, 3.5K, 10K, and 20K MWCOs. The dialysis flasks are available in distinct colors that correspond to the MWCO of the dialysis membrane: purple (20 kDa), orange (10 kDa), pink (3.5 kDa), and blue (2 kDa).
Use Slide-A-Lyzer dialysis flasks in various applications, including low molecular-weight contaminant and salt removal, buffer exchange, and sample concentration.
Additional features of Slide-A-Lyzer dialysis flasks:
- Ease-of-use—No knots or clips are needed to seal the units; simply pipette or pour sample into the flask and begin dialysis.
- High recovery—A rectangular flask design heightens recovery of the entire sample volume via the opening at the top of the flask.
Choose your dialysis flask
| MWCO | Volume | Description |
|---|---|---|
| 2K | 250 mL | Slide-A-Lyzer Dialysis Flasks, blue |
| 3.5K | 250 mL | Slide-A-Lyzer Dialysis Flasks, pink |
| 10K | 250 mL | Slide-A-Lyzer Dialysis Flasks, orange |
| 20K | 250 mL | Slide-A-Lyzer Dialysis Flasks, purple |
Slide-A-Lyzer Dialysis flasks make sample loading and recovery easy. Simply attach the supplied float-ring and hydrate membrane for 2 minutes, pour sample into device, remove air, and replace the cap. Dialyze for 8 hours to overnight. Replace buffer after 2 and 5 hours, then pour out sample to recover (Figure 3).
Figure 3. Slide-A-Lyzer dialysis flasks workflow.
Slide-A-Lyzer dialysis flasks video
View the step-by-step instructional video that shows how to use the dialysis flasks.
Dialysis devices
Slide-A-Lyzer MINI dialysis devices have a cup-like design that is available in 3 volume sizes: 0.1 mL devices, which can be placed into a foam float during dialysis, and 0.5 mL and 2 mL sizes, which are capped by inserting them into the included 15 mL and 50 mL conical tubes, respectively. The tubes serve as dialysis reservoirs for easy and self-contained dialysis and are available in 2K, 3.5K, 7K, 10K, and 20K MWCOs.
Use the Slide-A-Lyzer MINI Dialysis Devices in applications including low molecular weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, microconjugation reactions, and sample concentration.
Additional features of Slide-A-Lyzer MINI dialysis devices:
- High sample recovery—Low-binding membranes help ensure that proteins and other macromolecules are recovered.
- Ease-of-use—Disposable; no syringe adapters or other assembled equipment required.
- Chemical compatibility—Cellulose membranes are compatible with common chemicals and buffers.
Choose your MINI dialysis device based on volume and MWCO
The Slide-A-Lyzer MINI device makes dialyzing small volumes easy. Just add the sample, place the device in the conical tube (for 0.5 mL and 2 mL devices), recap, and shake for up to 4 hours or overnight. Use a pipet to recover the sample (Figure 1).
Figure 1. Slide-A-Lyzer MINI dialysis device workflow.
Table 1. Protein recovery percentage using the 2 mL MINI Dialysis Device.
| Membrane MWCO | Protein/Peptide | Recovery (%) |
|---|---|---|
| 3.5K | Insulin Chain B (3.5 kDa) | 90.13 |
| 10K | Cytochrome C (12.4 kDa) | 94.44 |
| 20K | Myoglobin (17 kDa) | 95 |
Insulin chain B, cytochrome C and myoglobin (0.25 mg/mL) in either 50 mM sodium phosphate, 75 mM NaCl at pH 7.2 or 0.2 M carbonate-bicarbonate buffer at pH 9.4 were dialyzed overnight (17 hours) at 4°C. The amount of protein in the retentate was determined using the Pierce BCA Protein Assay.
Slide-A-Lyzer MINI dialysis devices video
View the step-by-step instructional video that shows how to use the MINI dialysis devices.
Dialysis cassettes
Slide-A-Lyzer G3 dialysis cassettes accommodate volumes ranging from 1–125 mL, and are available in 2K, 3.5K, 10K, and 20K molecular weight cutoffs (MWCOs).
Use Slide-A-Lyzer G3 dialysis cassettes in various applications, including low molecular-weight contaminant and salt removal, buffer exchange, and sample concentration.
Additional features of the Slide-A-Lyzer G3 dialysis cassettes:
- Sample security—Sealed membranes prevent leakage that can occur with dialysis tubing.
- Ergonomic—A lighter and more compact frame enables easier handling, loading, and retrieval.
- Greener by design—The Slide-A-Lyzer G3 features up to a 68% reduction in plastic waste compared to previous generations while offering the same secure, high-performance dialysis. Refer to the Slide-A-Lyzer G3 green fact sheet for more information.
- Self-flotation—No external flotation device or clips are required.
Choose your G3 dialysis cassette based on volume and MWCO
The Slide-A-Lyzer G3 dialysis cassette is simple to use. Load the cassette with sample, put it into the dialysis buffer, and then retrieve the sample (Figure 2).
Figure 2. Slide-A-Lyzer G3 dialysis cassettes workflow.
Comparison of dialysis cassettes with traditional dialysis tubing
| | |
|---|---|---|
| Dialysis product | Traditional tubing | Cassette |
| Sample handling | Slippery when wet Flat tubing is difficult to handle when wet, increasing risk of sample loss during sample addition/removal | Easy handling and secure sample delivery Plastic frame and cap allow for secure sample addition/removal |
| Sample recovery | Risk of sample loss Sample can leak out if clamp becomes loose or falls off | Sample protection Welded membrane and leak-proof cap helps reduce the risk of sample loss |
| Speed | Time consuming Non-optimized design that typically requires overnight dialysis | Fast and efficient Innovative design with high surface/volume ratio allows for more rapid dialysis than conventional tubing |
Slide-A-Lyzer G3 dialysis cassettes video
View the step-by-step instructional video that shows how to use the G3 dialysis cassettes.
Dialysis flasks
Slide-A-Lyzer dialysis flasks facilitate simple and effective dialysis in larger sample volumes ranging from 150 mL up to 250 mL. These devices accomplish typical buffer exchange in 8–24 hours and are available in 2K, 3.5K, 10K, and 20K MWCOs. The dialysis flasks are available in distinct colors that correspond to the MWCO of the dialysis membrane: purple (20 kDa), orange (10 kDa), pink (3.5 kDa), and blue (2 kDa).
Use Slide-A-Lyzer dialysis flasks in various applications, including low molecular-weight contaminant and salt removal, buffer exchange, and sample concentration.
Additional features of Slide-A-Lyzer dialysis flasks:
- Ease-of-use—No knots or clips are needed to seal the units; simply pipette or pour sample into the flask and begin dialysis.
- High recovery—A rectangular flask design heightens recovery of the entire sample volume via the opening at the top of the flask.
Choose your dialysis flask
| MWCO | Volume | Description |
|---|---|---|
| 2K | 250 mL | Slide-A-Lyzer Dialysis Flasks, blue |
| 3.5K | 250 mL | Slide-A-Lyzer Dialysis Flasks, pink |
| 10K | 250 mL | Slide-A-Lyzer Dialysis Flasks, orange |
| 20K | 250 mL | Slide-A-Lyzer Dialysis Flasks, purple |
Slide-A-Lyzer Dialysis flasks make sample loading and recovery easy. Simply attach the supplied float-ring and hydrate membrane for 2 minutes, pour sample into device, remove air, and replace the cap. Dialyze for 8 hours to overnight. Replace buffer after 2 and 5 hours, then pour out sample to recover (Figure 3).
Figure 3. Slide-A-Lyzer dialysis flasks workflow.
Slide-A-Lyzer dialysis flasks video
View the step-by-step instructional video that shows how to use the dialysis flasks.
Resources for protein dialysis
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Stylesheet for Classic Wide Template adjustments
For Research Use Only. Not for use in diagnostic procedures.