Search
Automated Nucleic Acid Extraction with MagMAX Kits |
MagMAX automated extraction kits enable nucleic acid isolation for a wide range of applications. Utilizing magnetic bead-based technology, these kits include pre-filled buffers and reagents in vials, cartridges, or plates, and are designed to be paired with liquid handlers and purification systems for automated extraction. MagMAX kits efficiently purify DNA and RNA from various biological samples and work easily with Thermo Scientific KingFisher purification systems to help streamline workflows, help enhance reproducibility, and support both low and high-throughput needs.
Advantages of magnetic beads over other nucleic acid purification methods
- Higher purity and yields due to exceptional binding capacity thorough exposure to target molecules during mixing and washing steps, allowing for efficient capture and release
- Scalability through automation compatibility and high-throughput processing, facilitating large-scale research and clinical applications
- Streamlined workflow with standardized protocols involving fewer steps, enabling consistent and reproducible results with reduced processing time
- Greater sample range across samples that have different viscosities and processing requirements
- Gentle separation without the use of columns, filters, and excessive centrifugation minimizes potential clogging and helps prevent shearing of sensitive biomolecules
- Flexibility to target nucleic acids of specific fragment sizes required for downstream analysis of choice
- Improved elution even at small volumes as low as 1 mL
Applications for magnetic bead purification
Purification by sample type
Find the right kit, reagents, and protocol for your nucleic acid isolation
Magnetic bead genomic DNA extraction
Reliable DNA extraction is crucial for the success of genomic studies, as it helps ensure the integrity and purity of the DNA used in downstream applications. MagMAX DNA isolation kits help provide high-quality genomic DNA from a wide range of sample types, delivering yields and purity that meet the stringent requirements of advanced analytical methods. These kits facilitate efficient extraction, making the DNA suitable for various applications such as qPCR, next-generation sequencing, microarray analysis, and more.
The MagMAX DNA Multi-Sample Ultra 2.0 Kit is an automatable magnetic bead-based sample preparation technology for the isolation of genomic DNA (gDNA) from 50 µL to 2 mL of whole blood, saliva, buffy coat, and buccal swabs.
The MagMAX DNA Multi-Sample Ultra Kit is optimized to isolate genomic DNA (gDNA) from a variety of samples, such as whole blood, buccal cells, saliva, urine, blood cards, mouth rinse, and tissue.
Magnetic bead high molecular weight (HMW) DNA extraction
High molecular weight (HMW) DNA is most often used for long-read sequencing in structural variation studies. Reliable isolation methods are important to minimize fragmentation, help ensure purity, and maximize yield. The MagMAX HMW DNA Kit leverages magnetic bead technology that helps provide reliable and efficient isolation of high-integrity HMW DNA with efficiency and reproducibility for use in downstream long-read sequencing applications.
Designed for long-read sequencing applications, the MagMAX HMW DNA Kit isolates high-integrity DNA with large intact fragments > 100 kb from blood, cell, and tissue samples.
Plasmid DNA isolation
Plasmid DNA (pDNA) is one of the analytes that is at the forefront of scientific advancement as it is commonly used in innovative gene therapy treatments to the development of advanced vaccines. The MagMAX Pro HT NoSpin Plasmid Miniprep kit facilitates high-throughput and centrifuge-free purification of transfection-grade plasmid DNA for use in these types of applications. Specialized dual magnetic-bead technology helps increase yield and improve purity by precisely controlling the purification steps and reagent volumes. The purified plasmid DNA is well suited for use in a wide range of downstream applications, including transfection, vector preparation, enzymatic modifications, cloning, sequencing, and in vitro transcription/translation.
MagMAX Pro HT NoSpin Miniprep Plasmid Purification Kit
Specifically designed for high-throughput plasmid isolation, the MagMAX Pro HT NoSpin Plasmid Miniprep Kit provides a scalable and ultra-rapid approach for purifying high-quality, transfection-grade plasmid DNA (pDNA) directly from bacterial culture.
Magnetic bead RNA extraction
Accurate gene expression analysis and other RNA-based techniques rely on effective RNA isolation. Magnetic bead-based extraction methods offer significant advantages over traditional methods, including reduced contamination risk, improved yields and purity, and the ability to isolate RNA from a broader range of sample types. Magnetic bead isolation increases efficiency and minimizes hazardous materials, helping make the process safer. The MagMAX mirVana Total RNA Isolation Kit utilizes magnetic bead technology to enable reproducible recovery of high-quality total RNA, helping provide reliable and consistent results for downstream analyses.
Isolate total RNA, as well as small RNAs such as microRNA (miRNA), from serum/plasma, whole blood, tissue, cell culture, and urine using a phenol-free extraction.
Perform RT-qPCR gene expression analysis directly
from cultured cells without
extracting RNA
DNA and RNA extraction for liquid biopsy and cancer research
Nucleic acids are fundamental to cancer research, helping provide insights into genetic mutations, gene expression patterns, and molecular mechanisms underlying cancer development and progression. Magnetic bead DNA and RNA isolation using MagMAX kits designed for cancer research applications helps ensure reproducible recovery of high-quality nucleic acids from biological samples, enabling efficient purification that supports reliable downstream genomic analysis.
Cell-free DNA (cfDNA) and cell-free RNA (cfRNA) isolation
Isolating cell-free total nucleic acids (cfTNA) and cell-free DNA (cfDNA) can be challenging due to low abundance in samples and potential for gDNA contamination. MagMAX kits designed for cfTNA and cfDNA isolation utilize magnetic bead technology with optimized chemistries that help provide reliable isolation of cell free nucleic acids with high yield and purity from plasma, serum, and other biological fluids, enabling development of non-invasive cancer diagnostics, liquid biopsy applications, and cancer genomics studies.
Isolate and analyze circulating cell-free total nucleic acid (cfTNA) from plasma and other cell-free samples as a non-invasive method to analyze and monitor targets associated with certain disease states such as cancer.
Isolate and analyze circulating cfDNA from plasma, serum, or urine as a non-invasive approach to analyze and monitor targets associated with certain disease states such as cancer.
Sequential nucleic acid isolation from blood and bone marrow
Isolation of nucleic acids from hematological samples offers a view into identification of cancer-causing genetic alterations. The MagMAX Sequential DNA/RNA Kit uses magnetic bead purification technology to sequentially isolate high-quality genomic DNA and total RNA from a single whole blood or bone marrow sample for use in downstream molecular applications. This comprehensive approach can help provide insights into the various types of genetic abnormalities found within hematologic malignancies.
Isolate DNA and RNA from a single blood or bone marrow sample, yielding separate ready-to-use eluates for hematological cancer studies and other molecular applications.
Nucleic acid extraction from FFPE tissue samples
Formalin-Fixed Paraffin-Embedded (FFPE) tissue samples can be demanding to work with and require special processing prior to nucleic acid extraction, making streamlined workflows that are reliable and efficient especially important. The MagMAX FFPE DNA/RNA Ultra Kit allows for sequential isolation of DNA and RNA from the same FFPE tissue sample, yielding high-quality separate eluates. The kit is compatible with the solvent-free AutoLys deparaffinization system that allows for a fast deparaffinization process while minimizing tissue loss.
Recover sequential isolations of DNA and RNA from the same formaldehyde- or paraformaldehyde-fixed paraffin-embedded (FFPE) tissue sample.
Explore solutions for liquid biopsy biomarker isolation and analysis
Viral and pathogen nucleic acid isolation
MagMAX Viral/Pathogen kits utilize magnetic bead technology for efficient extraction of high-quality nucleic acids from microbes within a wide range of sample types. These kits offer high sensitivity and automated protocols for rapid processing, enabling accurate pathogen detection and analysis for infectious disease research and diagnostic development.
Recover nucleic acid from viral, bacterial (gram positive and gram negative), fungal, and parasitic pathogens from a variety of biological sample types such as swabs in transport media, saliva, stool, urine, plasma and serum.
Recover high quality nucleic acids from virus and gram-negative bacteria in samples such as blood, swabs, urine, and viral transport media (VTM).
Recover high quality nucleic acids from virus and gram-negative bacteria in samples such as blood, swabs, urine, and viral transport media (VTM) in bulk prep format.
Recover nucleic acid from viral particles, gram negative bacteria; as well as from the tough pathogens, such as gram-positive bacteria, yeast, and fungi with this comprehensive kit.
Learn more about nucleic acid isolation for infectious disease research
Looking for diagnostic solutions?
Explore our kit for viral nucleic acid isolation from human samples for clinical use
MagMAX magnetic bead purification by sample type
Microbial DNA isolation
Microbial DNA can be isolated and enriched to help researchers better understand how the microbiome influences human health. It is of utmost importance that the nucleic acids recovered accurately reflect the representation of the diverse microbes in the sample community. Our products offer high- and low-throughput purification of high-quality nucleic acids from a wide variety of sample types, including challenging samples such as stool and soil. Recovered nucleic acids are compatible with a broad range of applications, including microarrays, real-time PCR, and next-generation sequencing.
Enables efficient high-throughput nucleic acid extraction with optimized reagents designed for microbiome samples such as stool, soil, biofluids, swabs, and more.
Enables efficient low-throughput nucleic acid extraction with optimized reagents designed for microbiome samples such as stool, soil, biofluids, swabs, and more.
Wastewater testing and surveillance for infectious agents
Wastewater surveillance has recently gained significant attention as a useful tool for monitoring the regional spread of infectious disease, including the novel coronavirus and other airborne pathogens. MagMAX Wastewater Ultra Nucleic Acid Isolation Kits offer a whole host of advantages over traditional wastewater surveillance methods. They are easy to use, and you can isolate nucleic acids from concentrated wastewater in as little as 45 minutes. Our viral enrichment protocol using magnetic beads offers an efficient concentration and nucleic acid isolation.
High-throughput recovery of high-quality nucleic acids from wastewater samples compatible with a broad range of applications, including real-time PCR and next-generation sequencing.
High-throughput virus enrichment and nucleic acid extraction from wastewater samples compatible with a broad range of applications, including real-time PCR and next-generation sequencing.
DNA extraction from saliva
Saliva can be collected non-invasively and is a common source of DNA for research. Without compromising yield and purity, the MagMAX Saliva gDNA Isolation Kit uses a single step to minimize the time and effort typically required by other methods which may use multiple laborious sample processing steps. Process a broad range of sample volume inputs simultaneously without sample normalization or reagent volume adjustments for hassle-free sample prep.
Isolate genomic DNA from fresh and stabilized saliva ideal for applications such as qPCR, next-generation sequencing, and microarray analysis.
Guide for saliva collection and testing
Learn more about saliva-based research in our “How-To” Guide for Saliva Collection and Testing. This complex biofluid plays a critical role in human physiology and disease pathophysiology.
HIV-1 RNA isolation from dried blood spots
HIV-1 RNA can be isolated now from dried blood spots (DBS), a sample type that requires no specialized equipment for transport and can be shipped at ambient temperatures. DBS sampling is a cost-effective solution, with easy transportation protocols, that can extend the reach of global health and surveillance in remote populations. The MagMAX Viral/Pathogen Nucleic Acid Isolation Kit for HIV-1 Dried Blood Spots isolates and purifies nucleic acid from dried blood spots (DBS) or plasma. The MagMAX Viral/Pathogen Nucleic Acid Isolation Kit for HIV-1 Dried Blood Spots is compatible with KingFisher automated purification systems and downstream assays such as the HIV-1 Genotyping Kit with Integrase.
Recover high-quality HIV-1 viral RNA from dried blood spots (DBS) and plasma for HIV-1 drug resistance testing compatible with downstream applications such as RT-PCR and Sanger sequencing.
Genotyping workflow used to detect HIV drug resistance
Click image to enlarge
Steps in a typical genotyping workflow include specimen processing, RNA extraction, RT-PCR, nested PCR, confirmation, purification, cycle sequencing, sequencing clean-up, sequence detection, and data analysis.
Plant cell RNA and DNA extraction
Steps to extract DNA and RNA from plant samples includes the following: Disrupt the tissue and homogenize the plant sample, bind nucleic acid with target magnetic beads, wash and elute sample to purify DNA using a magnetic particle processing instrument or manually using microcentrifuge tubes.
Challenges of extracting DNA and RNA from plants can be difficult and require kits specifically designed to mitigate carbohydrates, phenolics, and other compounds found in plant tissues. Cell walls can be difficult to disrupt, and lysates often contain inhibiting compounds such as tannins, phenolics, and complex polysaccharides which may hinder DNA quality, affecting downstream reactions.
For automated high-throughput extraction or manual purification, the MagMAX Plant DNA Isolation Kit is designed to reduce these challenges and effectively extract DNA from a wide variety of plant species. The kit uses MagMAX magnetic bead technology and eliminates the need for phenol/chloroform extraction or alcohol precipitation. The procedures are optimized for the isolation of DNA from 10–100 mg plant samples. DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, age of tissue sample, and storage conditions.
Automated high-throughput or manual purification of DNA from a wide variety of plant species for use in a wide range of downstream applications such as PCR, qPCR, or next-generation sequencing.
Automated high-throughput or manual purification of total RNA from wide variety of plant species and tissue types for use in a wide range of downstream applications, such as RT-PCR, RT-qPCR, and other enzymatic reactions.
Microbial DNA isolation
Microbial DNA can be isolated and enriched to help researchers better understand how the microbiome influences human health. It is of utmost importance that the nucleic acids recovered accurately reflect the representation of the diverse microbes in the sample community. Our products offer high- and low-throughput purification of high-quality nucleic acids from a wide variety of sample types, including challenging samples such as stool and soil. Recovered nucleic acids are compatible with a broad range of applications, including microarrays, real-time PCR, and next-generation sequencing.
Enables efficient high-throughput nucleic acid extraction with optimized reagents designed for microbiome samples such as stool, soil, biofluids, swabs, and more.
Enables efficient low-throughput nucleic acid extraction with optimized reagents designed for microbiome samples such as stool, soil, biofluids, swabs, and more.
Wastewater testing and surveillance for infectious agents
Wastewater surveillance has recently gained significant attention as a useful tool for monitoring the regional spread of infectious disease, including the novel coronavirus and other airborne pathogens. MagMAX Wastewater Ultra Nucleic Acid Isolation Kits offer a whole host of advantages over traditional wastewater surveillance methods. They are easy to use, and you can isolate nucleic acids from concentrated wastewater in as little as 45 minutes. Our viral enrichment protocol using magnetic beads offers an efficient concentration and nucleic acid isolation.
High-throughput recovery of high-quality nucleic acids from wastewater samples compatible with a broad range of applications, including real-time PCR and next-generation sequencing.
High-throughput virus enrichment and nucleic acid extraction from wastewater samples compatible with a broad range of applications, including real-time PCR and next-generation sequencing.
DNA extraction from saliva
Saliva can be collected non-invasively and is a common source of DNA for research. Without compromising yield and purity, the MagMAX Saliva gDNA Isolation Kit uses a single step to minimize the time and effort typically required by other methods which may use multiple laborious sample processing steps. Process a broad range of sample volume inputs simultaneously without sample normalization or reagent volume adjustments for hassle-free sample prep.
Isolate genomic DNA from fresh and stabilized saliva ideal for applications such as qPCR, next-generation sequencing, and microarray analysis.
Guide for saliva collection and testing
Learn more about saliva-based research in our “How-To” Guide for Saliva Collection and Testing. This complex biofluid plays a critical role in human physiology and disease pathophysiology.
HIV-1 RNA isolation from dried blood spots
HIV-1 RNA can be isolated now from dried blood spots (DBS), a sample type that requires no specialized equipment for transport and can be shipped at ambient temperatures. DBS sampling is a cost-effective solution, with easy transportation protocols, that can extend the reach of global health and surveillance in remote populations. The MagMAX Viral/Pathogen Nucleic Acid Isolation Kit for HIV-1 Dried Blood Spots isolates and purifies nucleic acid from dried blood spots (DBS) or plasma. The MagMAX Viral/Pathogen Nucleic Acid Isolation Kit for HIV-1 Dried Blood Spots is compatible with KingFisher automated purification systems and downstream assays such as the HIV-1 Genotyping Kit with Integrase.
Recover high-quality HIV-1 viral RNA from dried blood spots (DBS) and plasma for HIV-1 drug resistance testing compatible with downstream applications such as RT-PCR and Sanger sequencing.
Genotyping workflow used to detect HIV drug resistance
Click image to enlarge
Steps in a typical genotyping workflow include specimen processing, RNA extraction, RT-PCR, nested PCR, confirmation, purification, cycle sequencing, sequencing clean-up, sequence detection, and data analysis.
Plant cell RNA and DNA extraction
Steps to extract DNA and RNA from plant samples includes the following: Disrupt the tissue and homogenize the plant sample, bind nucleic acid with target magnetic beads, wash and elute sample to purify DNA using a magnetic particle processing instrument or manually using microcentrifuge tubes.
Challenges of extracting DNA and RNA from plants can be difficult and require kits specifically designed to mitigate carbohydrates, phenolics, and other compounds found in plant tissues. Cell walls can be difficult to disrupt, and lysates often contain inhibiting compounds such as tannins, phenolics, and complex polysaccharides which may hinder DNA quality, affecting downstream reactions.
For automated high-throughput extraction or manual purification, the MagMAX Plant DNA Isolation Kit is designed to reduce these challenges and effectively extract DNA from a wide variety of plant species. The kit uses MagMAX magnetic bead technology and eliminates the need for phenol/chloroform extraction or alcohol precipitation. The procedures are optimized for the isolation of DNA from 10–100 mg plant samples. DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, age of tissue sample, and storage conditions.
Automated high-throughput or manual purification of DNA from a wide variety of plant species for use in a wide range of downstream applications such as PCR, qPCR, or next-generation sequencing.
Automated high-throughput or manual purification of total RNA from wide variety of plant species and tissue types for use in a wide range of downstream applications, such as RT-PCR, RT-qPCR, and other enzymatic reactions.
Automate MagMAX kits using KingFisher instruments
Benefits of KingFisher instruments include
- Various throughputs—Process 6–96 samples per run depending on the instrument model
- Interchangeable formats—Process a wide range of input volumes
- Protocol customization—Easily edit, modify, or create new protocols
- Optimized reagents—Compatible with multiple magnetic-bead reagents
Explore: KingFisher instruments
Download: Validated software automation scripts for KingFisher purification instruments
Resources
Support
For Research Use Only. Not for use in diagnostic procedures.
Stylesheet for Classic Wide Template adjustments