GeneArt Seamless Cloning & Gibson Assembly®

Tools for assembling multiple DNA Fragments to build large and seamless clones

GeneArt Seamless Cloning and GeneArt Gibson Assembly Kits allow for the simultaneous assembly of up to 15 DNA fragments to create precise, very large constructs with no unwanted sequences in highly efficient reactions. These cloning methods circumvent the need for multiple rounds of restriction enzyme analysis and digestion, DNA end-repair, de-phosphorylation, ligation, enzyme inactivation and clean-up, and loss of precious DNA saving 3-4 weeks versus traditional RE cloning methods.

Which seamless cloning kit is right for you?

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  Assemble up to 4 fragments Assemble up to 8 fragments Assemble up to 15 fragments
  GeneArt Seamless Cloning Enzyme Mix GeneArt Seamless Cloning & Assembly Kit GeneArt Seamless PLUS Cloning and Assembly Kit GeneArt Type IIs Assembly kits GeneArt Gibson Assembly HiFi and EX Kits
Cloning efficiency >90%
  • 90% - single 5 kb DNA
  • 70% - 4 Fragments, 1 kb each
  • 40% - 4 DNA Fragments, 2 kb each
>90%
  • 90% - 5 fragments up to 2 kb each (direct)
  • > 90% - 8 fragments totaling 10 kb (pre-cloned)
>90%
All-in-one enzyme/buffer mix          
Number of fragments cloned simultaneously Up to 4 Up to 4 Up to 4 Up to 8 Up to 15
Maximum assembled product size Up to 13 kb (with vector) Up to 13 kb (with vector) Up to 40 kb (with vector)
  • Up to 13 kb (with vector)
  • Up to 20 kb to lower efficiency
Up to 100 kb+ (with vector)
Time to clone multiple fragments Typically in half a day 1 day Typically in half a day Typically in half a day Typically in half a day
Assemble fragments w/o end sequence homology w/o PCR          

For assembly using S. cerevisae MaV203 see the GeneArt High-Order Genetic Assembly System.

Gibson Assembly® is a registered trademark of SGI-DNA, Inc. used under permission and license.