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MagMAX DNA Multi-Sample Ultra Kit
Extract high-quality DNA that is compatible with sequencing or array platforms, from a range of sample types
- Process 6 to 96 samples in 45 minutes with 5 minutes hands-on-time, 15 minute incubation and 25 minute run with KingFisher systems.
- Samples can also be processed manually using a magnetic stand.
- Choose from two protocols depending on your input volume; no need to adjust reagent ratios.
- Isolate gDNA from 50 µL–2 mL of sample using a simple protocol in SBS microtiter plates.
- Skip sample and buffer volume normalization steps for hassle-free processing.
- Download validated protocols for KingFisher purification instruments.
- Stand-alone MagMAX DNA Multi-Sample Ultra 2.0 reagent bottle options.
- Buy Ultra 2.0 pre-dispensed in sealed KingFisher plates for easier, faster, and more consistent DNA extractions.
| Sample type | Multiple, including whole blood, swabs, tissue, and more |
| Sample input volume | 50 µL–2 mL |
| Yield |
|
| Elution volume | 50 µL–1 mL |
| Total processing time | 6–96 samples in 30–45 min depending on sample type |
| Reactions (rxn) | 1 kit = 100 rxn of 50–400 µL volume or 200 rxn of <200 µL volume; bulk reagents sold separately |
| Shipping conditions | Room temperature |
| Cat. No. | A36570 |
Automate MagMax workflows with KingFisher systems
Use this kit with KingFisher automation to extract high-quality DNA that is compatible with sequencing or array platforms, from a range of sample types. Choose from multiple protocols, but only two options for either large or small volume inputs, without normalization.
Extract genomic DNA from a range of sample types with the MagMAX DNA Multi-Sample Ultra 2.0 Kit
Extract gDNA from 50 µL–2 mL of fresh and stabilized saliva, whole blood, buccal swabs, EDTA/Streck cfDNA tube buffy coat, and other biological sample types. Maximize gDNA yields with the MagMAX DNA Multi-Sample Ultra 2.0 Kit by treating samples with proteinase K (PK) and PK-enhancer followed by an effective lysis/binding solution to simultaneously lyse cells, denature proteins, and capture gDNA on the magnetic binding beads. Remove proteins, detergents, and other impurities from the bound nucleic acid with the Ultra 2.0 Wash I solution. This releases the gDNA from the beads using a low-TE elution solution.
Magnetic bead-based gDNA extraction with MagMAX
The MagMAX DNA Multi-Sample Ultra 2.0 Kit uses magnetic bead-based workflows and combines multiple sample processing steps together to reduce the effort traditionally required by other methods of nucleic acid extraction. Simplify your sample preparation by processing a wide range of sample volume inputs simultaneously without sample normalization or reagent volume adjustments. Simply match your sample volumes to one of three standard input workflows (50 µL to 400 µL, up to 1 mL, up to 2 mL) and follow the instructions for either semi-automated sample or manual or preparation.
Designed to integrate with KingFisher Purification System
Optimize your use of the MagMAX DNA Multi-Sample Ultra 2.0 Kit by using them with KingFisher systems. For moderate-throughput use with the KingFisher Duo Prime and for higher-throughput use the KingFisher Flex. Process up to 400 µL of sample volume in SBS 96-well deep-well microtiter plates using the KingFisher Flex system. For smaller sample batches, process 12 samples using the Duo Prime system. 1 to 2 mL sample input volumes are purified in SBS 24-well deep-well microtiter plates useable on either KingFisher Flex or Duo Prime systems. BindIt software scripts for automation on both Flex and Duo Prime systems can be downloaded and installed easily (see Protocols tab/section).
MagMAX reagents for larger high-throughput sample processing
MagMAX DNA Multi-Sample Ultra 2.0 Kit reagents are available as stand-alone bottled reagents and are perfect for larger high-throughput sample processing. The reagents are individually bottled in larger volumes. If purchasing a set of 6 stand-alone reagents, users will have sufficient volume of MagMAX DNA Multi-Sample Ultra 2.0 reagents to process two thousand 50-200 uL samples or one thousand 200–400 uL samples.
Performance data
Click image to enlarge
Figure 1. Linear recovery of gDNA from saliva. Genomic DNA (gDNA) was isolated from 150 µL, 500 µL, 2 mL, and 4 mL of freshly collected saliva from from two independent donors using the MagMAX DNA Multi-Sample Ultra 2.0 kit on the KingFisher Flex Magnetic Particle Handler. A NanoDrop 8000 spectrophotometer was used to calculate total gDNA yield (A), A260/A280 ratio, and A260/A230 ratio (B).
Click image to enlarge
Figure 2. Linear recovery of gDNA from buffy coat. Genomic DNA (gDNA) was isolated from 50 µL, 100 µL, and 200 µL of buffy coat from a K2-EDTA blood collection tube or a Streck cfDNA blood collection tube using the MagMAX DNA Multi-Sample Ultra 2.0 kit on the KingFisher Flex Magnetic Particle Handler. A NanoDrop 8000 spectrophotometer was used to calculate total gDNA yield (A), A260/A280 ratio, and A260/A230 ratio (B).
Click image to enlarge
Figure 3. Linear recovery of gDNA from whole blood. Genomic DNA (gDNA) was isolated from 50 µL, 100 µL, 200 µL, 400 µL, 1 mL, and 2 mL of whole blood from two independent donors using the MagMAX DNA Multi-Sample Ultra 2.0 kit on the KingFisher Flex Magnetic Particle Handler. A NanoDrop 8000 spectrophotometer was used to calculate total gDNA yield (A), A260/A280 ratio, and A260/A230 ratio (B). Inset diagram shows the linear recovery of total gDNA from smaller input volumes (50–400 µL) of whole blood from donor 1 and donor 2.
Protocol files
| Protocol | KingFisher Duo | KingFisher Flex |
|---|---|---|
| 1 mL script | MagMAX_Ultra2_1mL_V2_Duo.bdz | MagMAX_Ultra2_1mL_V2_Flex.bdz |
| 2 mL script | MagMAX_Ultra2_2mL_V2_Duo.bdz | MagMAX_Ultra2_2mL_V2_Flex.bdz |
| 400 µL script | MagMAX_Ultra2_400uL_V2_Duo.bdz | MagMAX_Ultra2_400uL_V2_Flex.bdz |
| Buffy coat script | MagMAX_Ultra2_buffy coat_V2_Duo.bdz | MagMAX_Ultra2_buffy coat_V2_Flex.bdz |
| 200 µL script | MagMAX_Ultra2_200uL_V2_Duo.bdz | MagMAX_Ultra2_200uL_V2_Flex.bdz |
| Direct script | MagMAX_Ultra2_Direct_V2_Duo.bdz | MagMAX_Ultra2_Direct_V2_Flex.bdz |
Choose between two automated, magnetic bead-based DNA kits
| MagMAX DNA Multi-Sample Ultra 2.0 Kit Optimized for blood, saliva, buffy coat, | MagMAX DNA Multi-Sample Ultra Kit Covers range of sample types | |
 |  | |
| Sample volumes |
|
|
| Elution volumes | 50 µL–1 mL | 50 µL–1 mL |
| Time per run |
|
|
| Automation/HT |
|
|
| Purification | PCR, NGS-ready purified gDNA | PCR, NGS-ready gDNA |
| Order now | Order now |
| Sample type | Multiple, including whole blood, swabs, tissue, and more |
| Sample input volume | 50 µL–2 mL |
| Yield |
|
| Elution volume | 50 µL–1 mL |
| Total processing time | 6–96 samples in 30–45 min depending on sample type |
| Reactions (rxn) | 1 kit = 100 rxn of 50–400 µL volume or 200 rxn of <200 µL volume; bulk reagents sold separately |
| Shipping conditions | Room temperature |
| Cat. No. | A36570 |
Automate MagMax workflows with KingFisher systems
Use this kit with KingFisher automation to extract high-quality DNA that is compatible with sequencing or array platforms, from a range of sample types. Choose from multiple protocols, but only two options for either large or small volume inputs, without normalization.
Extract genomic DNA from a range of sample types with the MagMAX DNA Multi-Sample Ultra 2.0 Kit
Extract gDNA from 50 µL–2 mL of fresh and stabilized saliva, whole blood, buccal swabs, EDTA/Streck cfDNA tube buffy coat, and other biological sample types. Maximize gDNA yields with the MagMAX DNA Multi-Sample Ultra 2.0 Kit by treating samples with proteinase K (PK) and PK-enhancer followed by an effective lysis/binding solution to simultaneously lyse cells, denature proteins, and capture gDNA on the magnetic binding beads. Remove proteins, detergents, and other impurities from the bound nucleic acid with the Ultra 2.0 Wash I solution. This releases the gDNA from the beads using a low-TE elution solution.
Magnetic bead-based gDNA extraction with MagMAX
The MagMAX DNA Multi-Sample Ultra 2.0 Kit uses magnetic bead-based workflows and combines multiple sample processing steps together to reduce the effort traditionally required by other methods of nucleic acid extraction. Simplify your sample preparation by processing a wide range of sample volume inputs simultaneously without sample normalization or reagent volume adjustments. Simply match your sample volumes to one of three standard input workflows (50 µL to 400 µL, up to 1 mL, up to 2 mL) and follow the instructions for either semi-automated sample or manual or preparation.
Designed to integrate with KingFisher Purification System
Optimize your use of the MagMAX DNA Multi-Sample Ultra 2.0 Kit by using them with KingFisher systems. For moderate-throughput use with the KingFisher Duo Prime and for higher-throughput use the KingFisher Flex. Process up to 400 µL of sample volume in SBS 96-well deep-well microtiter plates using the KingFisher Flex system. For smaller sample batches, process 12 samples using the Duo Prime system. 1 to 2 mL sample input volumes are purified in SBS 24-well deep-well microtiter plates useable on either KingFisher Flex or Duo Prime systems. BindIt software scripts for automation on both Flex and Duo Prime systems can be downloaded and installed easily (see Protocols tab/section).
MagMAX reagents for larger high-throughput sample processing
MagMAX DNA Multi-Sample Ultra 2.0 Kit reagents are available as stand-alone bottled reagents and are perfect for larger high-throughput sample processing. The reagents are individually bottled in larger volumes. If purchasing a set of 6 stand-alone reagents, users will have sufficient volume of MagMAX DNA Multi-Sample Ultra 2.0 reagents to process two thousand 50-200 uL samples or one thousand 200–400 uL samples.
Performance data
Click image to enlarge
Figure 1. Linear recovery of gDNA from saliva. Genomic DNA (gDNA) was isolated from 150 µL, 500 µL, 2 mL, and 4 mL of freshly collected saliva from from two independent donors using the MagMAX DNA Multi-Sample Ultra 2.0 kit on the KingFisher Flex Magnetic Particle Handler. A NanoDrop 8000 spectrophotometer was used to calculate total gDNA yield (A), A260/A280 ratio, and A260/A230 ratio (B).
Click image to enlarge
Figure 2. Linear recovery of gDNA from buffy coat. Genomic DNA (gDNA) was isolated from 50 µL, 100 µL, and 200 µL of buffy coat from a K2-EDTA blood collection tube or a Streck cfDNA blood collection tube using the MagMAX DNA Multi-Sample Ultra 2.0 kit on the KingFisher Flex Magnetic Particle Handler. A NanoDrop 8000 spectrophotometer was used to calculate total gDNA yield (A), A260/A280 ratio, and A260/A230 ratio (B).
Click image to enlarge
Figure 3. Linear recovery of gDNA from whole blood. Genomic DNA (gDNA) was isolated from 50 µL, 100 µL, 200 µL, 400 µL, 1 mL, and 2 mL of whole blood from two independent donors using the MagMAX DNA Multi-Sample Ultra 2.0 kit on the KingFisher Flex Magnetic Particle Handler. A NanoDrop 8000 spectrophotometer was used to calculate total gDNA yield (A), A260/A280 ratio, and A260/A230 ratio (B). Inset diagram shows the linear recovery of total gDNA from smaller input volumes (50–400 µL) of whole blood from donor 1 and donor 2.
Protocol files
| Protocol | KingFisher Duo | KingFisher Flex |
|---|---|---|
| 1 mL script | MagMAX_Ultra2_1mL_V2_Duo.bdz | MagMAX_Ultra2_1mL_V2_Flex.bdz |
| 2 mL script | MagMAX_Ultra2_2mL_V2_Duo.bdz | MagMAX_Ultra2_2mL_V2_Flex.bdz |
| 400 µL script | MagMAX_Ultra2_400uL_V2_Duo.bdz | MagMAX_Ultra2_400uL_V2_Flex.bdz |
| Buffy coat script | MagMAX_Ultra2_buffy coat_V2_Duo.bdz | MagMAX_Ultra2_buffy coat_V2_Flex.bdz |
| 200 µL script | MagMAX_Ultra2_200uL_V2_Duo.bdz | MagMAX_Ultra2_200uL_V2_Flex.bdz |
| Direct script | MagMAX_Ultra2_Direct_V2_Duo.bdz | MagMAX_Ultra2_Direct_V2_Flex.bdz |
Choose between two automated, magnetic bead-based DNA kits
| MagMAX DNA Multi-Sample Ultra 2.0 Kit Optimized for blood, saliva, buffy coat, | MagMAX DNA Multi-Sample Ultra Kit Covers range of sample types | |
| | |
| Sample volumes |
|
|
| Elution volumes | 50 µL–1 mL | 50 µL–1 mL |
| Time per run |
|
|
| Automation/HT |
|
|
| Purification | PCR, NGS-ready purified gDNA | PCR, NGS-ready gDNA |
| Order now | Order now |
Just like all Applied Biosystems MagMAX DNA Multi-Sample Ultra kits are optimized for KingFisher instruments.
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