MagMAX gDNA Saliva Isolation Kit

Isolate DNA from stabilized and freshly collected saliva

  • Process 6 to 96 samples in 25 minutes with 5 minutes hands-on-time, with KingFisher systems
  • Samples can also be processed manually using a magnetic stand
  • Isolate gDNA from 96 (200 – 500 µL) or 24 (0.5 – 2 mL) samples of saliva using simple protocols in SBS microtiter plates
  • Skip sample and buffer volume normalization steps for hassle-free processing
  • Automation-ready, phenol-free extraction
  • Compatible with multiple devices including microarray, PCFR and NGS downstream applications
  • Download validated software automation scripts for KingFisher purification instruments

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Product details

Sample type Saliva
Sample input volume 200 µL–2 mL
Yield 100ng/µL saliva on average (yield is dependent on each individual donor)
Elution volume 50 µL
Total processing time 25 min total time (5 min hands-on time)
Reactions 100 rxn, 500 rxn
Shipping condition Room temperature
Cat. No. A39059, A39060

Automation-ready

Use this kit with KingFisher automation to speed up your processing of saliva samples for genetic testing. Recover DNA that is compatible with Next-Generation Sequencing (NGS) and array platforms, from 96 samples, in 25 minutes.

Sample workflow

A single solution for DNA extraction from saliva samples

Why purchase multiple purification kits to isolate DNA present in your saliva sample types? Instead use our MagMAX Saliva gDNA Isolation Kit as a fully scalable for the isolation of genomic DNA (gDNA) from fresh and stabilized saliva. With magnetic bead-based technology at its core, the result is high yields of purified gDNA. Post sample preparation, downstream molecular biology applications include qPCR, microarray analysis, and next-generation sequencing. All components and reagents are available for purchase separately.

DNA Isolation from stabilized and freshly collected saliva

Extract gDNA from 200 µL–2 mL of stabilized or fresh saliva properly collected using the the MagMAX Saliva gDNA Isolation Kit. The workflow is compatible with saliva collection devices like the Oragene® from Genotek, the GeneFiX™ from IsoHelix, and the SalivaGard® from Biomatrica, the MagMAX Saliva gDNA isolation kit.

Simultaneously lyse cells, denature proteins, and capture gDNA on high-capacity magnetic binding beads with an innovative lysis/binding solution in the MagMAX Saliva gDNA Isolation Kit. Remove proteins, detergents, and other impurities from the bound nucleic acid with the Saliva gDNA Wash I Solution. Released the extracted gDNA from the beads using a low-TE elution solution.

Improve your workflow with magnetic bead-based technology

Combine multiple time-consuming sample processing steps in one single step with the MagMAX Saliva gDNA Isolation Kit. Magnetic bead-based technology reduces the laborious tasks often associated with other methods of nucleic acid extraction. Process a wide range of sample volume inputs concurrently without sample normalization or reagent volume adjustments. Simply match your saliva input volumes to the standard input workflows (50 µL to 500 µL, up to 1 mL, and up to 2 mL) and follow the directions for semi-automated sample preparation using the KingFisher Duo Prime or KingFisher Flex purification systems.

Process high-throughput and low-throughput samples with KingFisher Systems

The MagMAX Saliva gDNA Isolation Kit is optimized for moderate-throughput workflows using KingFisher Duo Prime while KingFisher Flex is used for higher-throughput sample processing. Process up to 500 µL of saliva in high-throughput capacity SBS 96-well deep-well microtiter plates with KingFisher Flex system or smaller sample batches, of 12 using the Duo Prime system. Dealing with larger sample input volumes for >500 µL and 1–2 mL? Use the higher capacity 24-well deep-well SBS plates for 24 samples on the Flex or 6 samples on the Duo Prime system. BindIt software scripts for both KingFisher instrument Flex and Duo Prime can be downloaded and installed on the instruments.

Fast processing time compared to competitor kits

The MagMAX Saliva gDNA Isolation kit has a total processing time of 25 minutes, including 5 minutes hands-on time compared to other supplier kits which typically take anywhere from 30+ minutes to 50 minutes for processing.

magmax-gdna-saliva-isolation-kit-speed

Figure 1. Fast processing time compared to competitor kits.

Performance data for MagMAX Saliva gDNA kits

Figure 4. Compatibility with saliva collection devices. 500 µL of stabilized saliva collected using the Oragene™ Discover, GeneFix™, or SalivaGard™ saliva collection devices was used as sample input for the MagMAX Saliva gDNA Isolation Kit. Total yield and concentration were analyzed using the Qubit Broad Range Assay or the RNAseP Verifiation Assay to quantitate genomic copies of the human RNaseP gene.

Figure 5. Performance compared to Competitor Q saliva gDNA isolation kit. A MagMAX Saliva gDNA Isolation Kit and Supplier Q recommended saliva gDNA isolation kit were used to isolate gDNA from 500 µL of saliva from six different donors. Total gDNA yield was determined using the Qubit dsDNA Broad Range Assay. Purity was determined by calculating the A260/A280 ratio using a Nanodrop spectrophotometer.

Figure 6. Performance compared to competitor saliva gDNA isolation kits. A MagMAX Saliva gDNA Isolation Kit and Supplier P and Supplier N recommended saliva gDNA isolation kits were used to isolate gDNA from 500 µL of saliva from six different donors. Total gDNA yield was determined using the Qubit dsDNA Broad Range Assay. TaqMan Assays for human actin B (ACTB) and GAPDH genes were used to assess the ability of the gDNA to amplify in qPCR reactions. The MagMAX Saliva gDNA Isolation Kit provided the lowest CT values for GAPDH across all six donors, indicating the presence of more amplifyable gDNA in samples prepared with the MagMAX kit. Purity was determined by calculating the A260/A280 ratio using a Nanodrop spectrophotometer.

Number of preps per kit

Figure 6. Number of preps per kit. The MagMAX Saliva gDNA isolation kit will scale in proportion to the volume of saliva to be processed. The table shows the number of 500 mL preparations of saliva that can be processed with each kit format or stand-alone reagents.

Product details

Sample type Saliva
Sample input volume 200 µL–2 mL
Yield 100ng/µL saliva on average (yield is dependent on each individual donor)
Elution volume 50 µL
Total processing time 25 min total time (5 min hands-on time)
Reactions 100 rxn, 500 rxn
Shipping condition Room temperature
Cat. No. A39059, A39060

Automation-ready

Use this kit with KingFisher automation to speed up your processing of saliva samples for genetic testing. Recover DNA that is compatible with Next-Generation Sequencing (NGS) and array platforms, from 96 samples, in 25 minutes.

Sample workflow

A single solution for DNA extraction from saliva samples

Why purchase multiple purification kits to isolate DNA present in your saliva sample types? Instead use our MagMAX Saliva gDNA Isolation Kit as a fully scalable for the isolation of genomic DNA (gDNA) from fresh and stabilized saliva. With magnetic bead-based technology at its core, the result is high yields of purified gDNA. Post sample preparation, downstream molecular biology applications include qPCR, microarray analysis, and next-generation sequencing. All components and reagents are available for purchase separately.

DNA Isolation from stabilized and freshly collected saliva

Extract gDNA from 200 µL–2 mL of stabilized or fresh saliva properly collected using the the MagMAX Saliva gDNA Isolation Kit. The workflow is compatible with saliva collection devices like the Oragene® from Genotek, the GeneFiX™ from IsoHelix, and the SalivaGard® from Biomatrica, the MagMAX Saliva gDNA isolation kit.

Simultaneously lyse cells, denature proteins, and capture gDNA on high-capacity magnetic binding beads with an innovative lysis/binding solution in the MagMAX Saliva gDNA Isolation Kit. Remove proteins, detergents, and other impurities from the bound nucleic acid with the Saliva gDNA Wash I Solution. Released the extracted gDNA from the beads using a low-TE elution solution.

Improve your workflow with magnetic bead-based technology

Combine multiple time-consuming sample processing steps in one single step with the MagMAX Saliva gDNA Isolation Kit. Magnetic bead-based technology reduces the laborious tasks often associated with other methods of nucleic acid extraction. Process a wide range of sample volume inputs concurrently without sample normalization or reagent volume adjustments. Simply match your saliva input volumes to the standard input workflows (50 µL to 500 µL, up to 1 mL, and up to 2 mL) and follow the directions for semi-automated sample preparation using the KingFisher Duo Prime or KingFisher Flex purification systems.

Process high-throughput and low-throughput samples with KingFisher Systems

The MagMAX Saliva gDNA Isolation Kit is optimized for moderate-throughput workflows using KingFisher Duo Prime while KingFisher Flex is used for higher-throughput sample processing. Process up to 500 µL of saliva in high-throughput capacity SBS 96-well deep-well microtiter plates with KingFisher Flex system or smaller sample batches, of 12 using the Duo Prime system. Dealing with larger sample input volumes for >500 µL and 1–2 mL? Use the higher capacity 24-well deep-well SBS plates for 24 samples on the Flex or 6 samples on the Duo Prime system. BindIt software scripts for both KingFisher instrument Flex and Duo Prime can be downloaded and installed on the instruments.

Fast processing time compared to competitor kits

The MagMAX Saliva gDNA Isolation kit has a total processing time of 25 minutes, including 5 minutes hands-on time compared to other supplier kits which typically take anywhere from 30+ minutes to 50 minutes for processing.

magmax-gdna-saliva-isolation-kit-speed

Figure 1. Fast processing time compared to competitor kits.

Performance data for MagMAX Saliva gDNA kits

Figure 4. Compatibility with saliva collection devices. 500 µL of stabilized saliva collected using the Oragene™ Discover, GeneFix™, or SalivaGard™ saliva collection devices was used as sample input for the MagMAX Saliva gDNA Isolation Kit. Total yield and concentration were analyzed using the Qubit Broad Range Assay or the RNAseP Verifiation Assay to quantitate genomic copies of the human RNaseP gene.

Figure 5. Performance compared to Competitor Q saliva gDNA isolation kit. A MagMAX Saliva gDNA Isolation Kit and Supplier Q recommended saliva gDNA isolation kit were used to isolate gDNA from 500 µL of saliva from six different donors. Total gDNA yield was determined using the Qubit dsDNA Broad Range Assay. Purity was determined by calculating the A260/A280 ratio using a Nanodrop spectrophotometer.

Figure 6. Performance compared to competitor saliva gDNA isolation kits. A MagMAX Saliva gDNA Isolation Kit and Supplier P and Supplier N recommended saliva gDNA isolation kits were used to isolate gDNA from 500 µL of saliva from six different donors. Total gDNA yield was determined using the Qubit dsDNA Broad Range Assay. TaqMan Assays for human actin B (ACTB) and GAPDH genes were used to assess the ability of the gDNA to amplify in qPCR reactions. The MagMAX Saliva gDNA Isolation Kit provided the lowest CT values for GAPDH across all six donors, indicating the presence of more amplifyable gDNA in samples prepared with the MagMAX kit. Purity was determined by calculating the A260/A280 ratio using a Nanodrop spectrophotometer.

Number of preps per kit

Figure 6. Number of preps per kit. The MagMAX Saliva gDNA isolation kit will scale in proportion to the volume of saliva to be processed. The table shows the number of 500 mL preparations of saliva that can be processed with each kit format or stand-alone reagents.

Ordering information

KingFisher Instruments

Just like all Applied Biosystems MagMAX kits, the gDNA saliva kits are optimized for KingFisher instruments.

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