MagMAX mirVana Total RNA Isolation Kit

Scalable isolation of RNA and microRNA from a variety of samples

  • Process 6 to 96 samples at once with KingFisher systems
  • Samples can also be processed manually using a magnetic stand
  • Isolate total RNA, as well as small RNAs such as microRNA (miRNA), from serum/plasma, whole blood, tissue, cell culture and urine using a phenol-free extraction (no organic extraction)
  • Streamline protocols for numerous non-invasive biological samples as well as tissues and cell cultures
  • Recover pure miRNA compatible with miRNA-Seq and qRT-PCR methods such as the TaqMan Advanced miRNA cDNA Synthesis Kit and other TaqMan advanced miRNA assays
  • Download validated protocols for KingFisher purification instruments
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Product details

Sample type Multiple, including plasma/serum, whole blood, tissue, cell culture, urine
Sample input volume
  • 50 µL of blood
  • up to 50 mg of tissue
  • 1 x 106 cells
  • 100 µL of plasma or serum
  • 250 µL of urine
Yield
  • 100 ng RNA from whole blood (50 µL) 
  • 600 ng from 5 mg mouse brain 
  • 2.5 µg from 5 mg mouse liver
Elution volume 50 µL
Total processing time 1.5–2 hr total time (20 min hands-on time)
Reactions (rxn) 96 rxn
Shipping Condition Room Temperature
Kit contents:  Box 1 (store at -5 to -30°C):
  • 1 x 0.48 mL Proteinase K, 50 mg/mL
  • 1 x 0.96 mL Lysis/Binding Enhancer
  • 1 x 0.2 mL TURBO DNase™, 20U/uL
Box 2 (store at room temperature):
  • 1 x 115 mL Lysis Buffer
  • 1 x 4.4 mL PK Digestion Buffer
  • 1 x 2 mL RNA Binding Beads
  • 1 x 20 mL Wash Solution 1 Concentrate
  • 1 x 60 mL Wash Solution 2 Concentrate
  • 1 x 4.8 mL Rebinding Buffer
  • 1 x 4.6 mL MagMAX TURBO DNase Buffer
  • 1 x 9.6 mL Elution Buffer
  • 1 Processing Plate
  • 2 Elution Plates
  • 4 Plate Covers
Cat. No. A27828

Automation-ready

Use this kit with KingFisher automation to extract and purify RNA. The kit is designed for both low- and high-throughput isolation of true total RNA that covers the full range of RNAs present in cells, from small RNAs such as microRNA, to noncoding RNA, messenger RNA, and ribosomal RNA.

Sample workflow

Extract total RNA from a wide range of sample types with MagMAX MirVana

Eliminate the headache of using “specialized” kits for each sample type by using a single solution with the MagMAX mirVana Total RNA Isolation Kit. Capable of processing a wide range of sample types, the kit is designed for both low- and high-throughput extraction of true total RNA. True total RNA includes the full range of RNAs present in cells such as microRNA, to noncoding RNA, messenger RNA or mRNA, and ribosomal RNA or rRNA. The phenol-free, magnetic bead format allows for automated RNA isolation workflows from the following samples:

Compatible Sample Types:

  • Serum/plasma
  • Whole blood
  • Tissue
  • Cell culture
  • Urine

MagMAX magnetic bead–based technology for nucleic acid extraction

MagMAX products are designed to provide consistent and dependable nucleic acid extraction using silica–magnetic particle technology. Magnetic beads offer many benefits for isolating RNA from cells, tissue, or whole blood, and viral RNA from cell-free samples.

Technology at the core of the MagMAX:

  • Offers higher RNA yields and more consistent results
  • Binds RNA with greater efficiency than silica-membrane methods
  • Elute RNA in as little as 20 µL of nuclease-free water to get more concentrated RNA

Video: How to isolate total RNA using magnetic bead–based technology

Learn how to achieve rapid, quantitative isolation of total RNA in both manual and automated workflows with the MagMAX mirVana Total RNA Isolation Kit.

Performance data

Figure 1. RNA quality on the Agilent Bioanalyzer. Total RNA, including small RNA, was isolated from 6 different tissue types (A: Mouse Brain; B: Mouse Heart; C: Mouse Liver; D: Mouse Spleen; E: HeLa cells; and F: Jurkat cells) using two different isolations methods and kits. Results shown in blue are for RNA isolated using the mirVana miRNA Isolation Kit and results shown in red are for RNA isolated using the MagMAX-96 mirVana Total RNA Isolation Kit that provides equivalent or improved results.

Figure 2. qRT-PCR results for tissue and cells. Total RNA, including small RNA, was isolated from mouse tissue and cells using the mirVana miRNA Isolation Kit or the MagMAX-96 mirVana Total RNA Isolation Kit. For the MagMAX-96 mirVana kit, RNA was isolated manually or using one of our automated instruments (MagMAX Express-96, KingFisher-Flex, or KingFisher-Duo).

For qRT-PCR analylsis, cDNA was generated using equal volumes RNA input from each sample (diluted 1:10 for miRNA) and the High-Capacity cDNA Reverse Transcription Kit for mRNA and the TaqMan MicroRNA Reverse Transcription Kit and specific primers for miRNA. qPCR reactions were then performed on 2 µL of cDNA using specific TaqMan Assays and TaqMan Universal Master Mix II (no UNG). Ct values were averaged and then graphed by target.

Figure 3. Results with TaqMan Advanced miRNA System. Total RNA, including small RNA, was isolated from mouse liver, HeLa cells, and human plasma using the mirVana miRNA Isolation Kit, mirVana PARIS miRNA Isolation Kit, or MagMAX-96 mirVana Total RNA Isolation Kit.

For qRT-PCR analylsis, cDNA was generated using equal volumes RNA input from each sample (diluted 1:10 for liver and HeLa) and the TaqMan Advanced cDNA Synthesis Kit and protocol. qPCR reactions were then performed on 2 µL of cDNA using the specific TaqMan Advanced miRNA Assays and TaqMan FAST Advanced Master Mix and fast cycling conditions. Ct values were averaged and then graphed by target.

Figure 4. qRT-PCR results for biofluids. Total RNA, including small RNA, was isolated from serum, plasma, and urine using the mirVana PARIS kit or the MagMAX-96 mirVana Total RNA Isolation Kit. For the MagMAX-96 mirVana kit, RNA was isolated manually or using one of our automated instruments (MagMAX Express-96, KingFisher-Flex, or KingFisher-Duo).

For qRT-PCR analylsis, cDNA was generated using equal volumes RNA input from each sample and the High-Capacity cDNA Reverse Transcription Kit for mRNA and the TaqMan MicroRNA Reverse Transcription Kit and specific primers for miRNA. qPCR reactions were then performed on 2 µL of cDNA using specific TaqMan Assays and TaqMan Universal Master Mix II (no UNG). Ct values were averaged and then graphed by target.

Protocols

Nucleic acid Sample type Kit name SKU Format Software protocol
RNA/
microRNA
Tissue, cells
blood, plasma, urine
MagMAX mirVana Total RNA A27828 96 preps MagMAX mirVana tissue cells
Duo PrimeFlex

MagMAX mirVana biofluids
Duo PrimeFlex

Product details

Sample type Multiple, including plasma/serum, whole blood, tissue, cell culture, urine
Sample input volume
  • 50 µL of blood
  • up to 50 mg of tissue
  • 1 x 106 cells
  • 100 µL of plasma or serum
  • 250 µL of urine
Yield
  • 100 ng RNA from whole blood (50 µL) 
  • 600 ng from 5 mg mouse brain 
  • 2.5 µg from 5 mg mouse liver
Elution volume 50 µL
Total processing time 1.5–2 hr total time (20 min hands-on time)
Reactions (rxn) 96 rxn
Shipping Condition Room Temperature
Kit contents:  Box 1 (store at -5 to -30°C):
  • 1 x 0.48 mL Proteinase K, 50 mg/mL
  • 1 x 0.96 mL Lysis/Binding Enhancer
  • 1 x 0.2 mL TURBO DNase™, 20U/uL
Box 2 (store at room temperature):
  • 1 x 115 mL Lysis Buffer
  • 1 x 4.4 mL PK Digestion Buffer
  • 1 x 2 mL RNA Binding Beads
  • 1 x 20 mL Wash Solution 1 Concentrate
  • 1 x 60 mL Wash Solution 2 Concentrate
  • 1 x 4.8 mL Rebinding Buffer
  • 1 x 4.6 mL MagMAX TURBO DNase Buffer
  • 1 x 9.6 mL Elution Buffer
  • 1 Processing Plate
  • 2 Elution Plates
  • 4 Plate Covers
Cat. No. A27828

Automation-ready

Use this kit with KingFisher automation to extract and purify RNA. The kit is designed for both low- and high-throughput isolation of true total RNA that covers the full range of RNAs present in cells, from small RNAs such as microRNA, to noncoding RNA, messenger RNA, and ribosomal RNA.

Sample workflow

Extract total RNA from a wide range of sample types with MagMAX MirVana

Eliminate the headache of using “specialized” kits for each sample type by using a single solution with the MagMAX mirVana Total RNA Isolation Kit. Capable of processing a wide range of sample types, the kit is designed for both low- and high-throughput extraction of true total RNA. True total RNA includes the full range of RNAs present in cells such as microRNA, to noncoding RNA, messenger RNA or mRNA, and ribosomal RNA or rRNA. The phenol-free, magnetic bead format allows for automated RNA isolation workflows from the following samples:

Compatible Sample Types:

  • Serum/plasma
  • Whole blood
  • Tissue
  • Cell culture
  • Urine

MagMAX magnetic bead–based technology for nucleic acid extraction

MagMAX products are designed to provide consistent and dependable nucleic acid extraction using silica–magnetic particle technology. Magnetic beads offer many benefits for isolating RNA from cells, tissue, or whole blood, and viral RNA from cell-free samples.

Technology at the core of the MagMAX:

  • Offers higher RNA yields and more consistent results
  • Binds RNA with greater efficiency than silica-membrane methods
  • Elute RNA in as little as 20 µL of nuclease-free water to get more concentrated RNA

Video: How to isolate total RNA using magnetic bead–based technology

Learn how to achieve rapid, quantitative isolation of total RNA in both manual and automated workflows with the MagMAX mirVana Total RNA Isolation Kit.

Performance data

Figure 1. RNA quality on the Agilent Bioanalyzer. Total RNA, including small RNA, was isolated from 6 different tissue types (A: Mouse Brain; B: Mouse Heart; C: Mouse Liver; D: Mouse Spleen; E: HeLa cells; and F: Jurkat cells) using two different isolations methods and kits. Results shown in blue are for RNA isolated using the mirVana miRNA Isolation Kit and results shown in red are for RNA isolated using the MagMAX-96 mirVana Total RNA Isolation Kit that provides equivalent or improved results.

Figure 2. qRT-PCR results for tissue and cells. Total RNA, including small RNA, was isolated from mouse tissue and cells using the mirVana miRNA Isolation Kit or the MagMAX-96 mirVana Total RNA Isolation Kit. For the MagMAX-96 mirVana kit, RNA was isolated manually or using one of our automated instruments (MagMAX Express-96, KingFisher-Flex, or KingFisher-Duo).

For qRT-PCR analylsis, cDNA was generated using equal volumes RNA input from each sample (diluted 1:10 for miRNA) and the High-Capacity cDNA Reverse Transcription Kit for mRNA and the TaqMan MicroRNA Reverse Transcription Kit and specific primers for miRNA. qPCR reactions were then performed on 2 µL of cDNA using specific TaqMan Assays and TaqMan Universal Master Mix II (no UNG). Ct values were averaged and then graphed by target.

Figure 3. Results with TaqMan Advanced miRNA System. Total RNA, including small RNA, was isolated from mouse liver, HeLa cells, and human plasma using the mirVana miRNA Isolation Kit, mirVana PARIS miRNA Isolation Kit, or MagMAX-96 mirVana Total RNA Isolation Kit.

For qRT-PCR analylsis, cDNA was generated using equal volumes RNA input from each sample (diluted 1:10 for liver and HeLa) and the TaqMan Advanced cDNA Synthesis Kit and protocol. qPCR reactions were then performed on 2 µL of cDNA using the specific TaqMan Advanced miRNA Assays and TaqMan FAST Advanced Master Mix and fast cycling conditions. Ct values were averaged and then graphed by target.

Figure 4. qRT-PCR results for biofluids. Total RNA, including small RNA, was isolated from serum, plasma, and urine using the mirVana PARIS kit or the MagMAX-96 mirVana Total RNA Isolation Kit. For the MagMAX-96 mirVana kit, RNA was isolated manually or using one of our automated instruments (MagMAX Express-96, KingFisher-Flex, or KingFisher-Duo).

For qRT-PCR analylsis, cDNA was generated using equal volumes RNA input from each sample and the High-Capacity cDNA Reverse Transcription Kit for mRNA and the TaqMan MicroRNA Reverse Transcription Kit and specific primers for miRNA. qPCR reactions were then performed on 2 µL of cDNA using specific TaqMan Assays and TaqMan Universal Master Mix II (no UNG). Ct values were averaged and then graphed by target.

Protocols

Nucleic acid Sample type Kit name SKU Format Software protocol
RNA/
microRNA
Tissue, cells
blood, plasma, urine
MagMAX mirVana Total RNA A27828 96 preps MagMAX mirVana tissue cells
Duo PrimeFlex

MagMAX mirVana biofluids
Duo PrimeFlex

Ordering information

KingFisher Instruments

Just like all Applied Biosystems MagMAX kits, the mirVana total RNA isolation kits are optimized for KingFisher instruments.

Resources
Support

Nucleic Acid Purification and Analysis Support Center
Find tips, troubleshooting help, and resources for your nucleic acid purification & analysis applications.