Choosing the right thermal cycler (also known as a thermocycler or PCR machine) is key to the success of your PCR experiments across many applications, including sequencing, genotyping, cloning, and mutagenesis. Applied Biosystems thermal cyclers help generate precise, consistent, reproducible results for every challenge, experiment, and budget.
Our thermocyclers are designed for reliability, accuracy, and user friendliness to fit scientists’ needs. Applied Biosystems PCR instruments are equipped with:
|ProFlex PCR System||VeritiPro Thermal Cycler||SimpliAmp Thermal Cycler||MiniAmp Thermal Cyclers||Automated Thermal Cycler (ATC)|
|Block formats||3 x 32-well 0.2 mL|
96-well 0.2 mL
2 x 96-well 0.2 mL
2 x flat block
2 x 384-well 0.02 mL
96-well 0.2 mL
384-well 0.02 mL
|96-well 0.2 mL||96-well 0.2 mL||96-well 0.2 mL|
384-well 0.02 mL
|Temperature optimization||6-zone VeriFlex Block on 96-well system|
2-zone VeriFlex Block on 32-well system
|6-zone VeriFlex Block on 96-well systems||3-zone VeriFlex Block on 96-well systems||3-zone VeriFlex Block on MiniAmp Plus Thermal Cycler||None|
|User interface||8.4 inch touch screen||8.0 inch touch screen||8.0 inch touch screen||5.0 inch touch screen||Via robotic platform or desktop software|
|768 reactions (384 x 2)||384 reactions||96 reactions||96 reactions||384 reactions|
|Max block ramp rate*||6.0°C/sec||6.0°C/sec||4.0°C/sec||3.5°C/sec||3.5°C/sec|
Applied Biosystems thermal cyclers that use VeriFlex technology are constructed with three or more separate heating/cooling elements below segmented metal blocks, each completely insulated from the others. This helps prevent heat interactions, providing the ability to set up to six different temperatures and allow for better-than-gradient control of temperature optimization.
Benefits of VeriFlex technology
Simulation mode supports easy transition and mimics ramp rates from old thermal cyclers to Applied Biosystems thermal cyclers, eliminating the need of re-optimization.
Simulation modes available for our thermal cycler models range from Bio-Rad™ T100, MJ Research PTC 200, Bio-Rad™ C1000, Bio-Rad™ MyCycler™, Eppendorf™ Mastercycler™, Applied Biosystems 9700, Applied Biosystems 2720, Applied Biosystems Veriti systems, and many more. Please visit each thermal cycler model for more details on the available simulation modes.
View and control the Applied Biosystems thermal cyclers in your facility with Applied Biosystems Thermal Cycler Fleet Control Software.
Fleet Control Software comes pre-installed on a server that sits securely behind your firewall giving you the security you need.
Your thermal cycler fleet control can be as restrictive or open as your work requires. Set up permissions in the custom rules menu for methods, users, and instruments. Restrict methods to specific instruments or users, or limit user access to specific methods or instruments.
Design protocols from anywhere; easily maintain run reports.
Develop protocols on any desktop computer or mobile device and store them in your secure personal cloud account. Once your run is prepared, start it from your instrument or remotely with Thermo Fisher Connect. After each run, the run report will be conveniently saved in your account.
Check run status and instrument availability in real-time
Download our Instrument Connect remote monitoring app to:
Discretionary access to project folders and protocols.
Share your protocols or other files with other Thermo Fisher Connect users on campus and around the world. It’s easy, convenient, and protected by the highest levels of privacy standards.
For peace of mind, the Thermo Fisher Cloud has high industry-standard security, so your work remains secure. We have partnered with Amazon Web Services (AWS) to offer a robust platform.
Applied Biosystems PCR plastics have been designed and validated to work with our thermal cyclersfor more than 25 years. Options for every format and throughput need are available to ensureconsistent, precise results for end-point PCR applications. Quickly find the right fit and compatible plastics for your desired model and block formats by using our plastics selection guide.
PCR and cDNA Synthesis Support Center
Tips and troubleshooting help
School of Molecular Biology, PCR Education
Technical content for new and experienced molecular biologists
We provide a full range of service options for all Applied Biosystems thermal cyclers.
View service plans
For Research Use Only. Not for use in diagnostic procedures.