Numerous techniques are available for the isolation of cellular proteins based on their post-translational modification (PTM) or active binding site. Protein enrichment is essential for isolating low-abundant proteins or isolating protein classes. Enrichment of specific proteins or protein complexes can most easily be accomplished using target-specific immobilized antibody or PTM specific affinity binding. The novel Thermo Scientific™ ActivX™ probes and enrichment kits enable the specific targeting of several enzyme classes, including kinases, GTPases, and serine hydrolases.
- Comprehensive—reagents and kits for multiple protein enrichment strategies
- Optimized—all reagents and kits are designed to maximize protein yield and reduce nonspecific binding
- Convenient—easy-to-use spin formats enable more rapid enrichment of proteins
- Flexible—kits can be used with either cultured mammalian cells or tissues
Choose the right protein enrichment kit for your application
protein Enrichment Kit
|Glycoprotein Enrichment Kit, ConA||Glycoprotein Enrichment Kit, WGA||Ubiqitin Enrichment Kit||Kinase Enrichment Kit with ATP Probe||ActivX™ TAMRA-FP Serine Hydrolase Probe|
|Target||Phosphorylated proteins||α-linked mannose and terminal glucose residues||N-acetyl glucosamine (GlcNAc)||Polyubiquin-ated proteins||ATP binding sites||Active serine hydrolase enzymes|
|Metal chelate affinity to phosphate groups||Lectin affinity to sugars||Lectin affinity to sugars||Antibody affinity to target||Biotinylated ATP analog to active-site lysine||TAMRA-labeled fluoro
phosphanate binds to active site serine
|Processing time||2 hr||<1 hr||<1 hr||<45 min||6.5 hr||3 hr|
|Enrichment format||IMAC-agarose||Immobilized conA-lectin-agarose||Immobilized WGA-lectin-agarose||Immobilized polyubiqitin affinity-agarose||Immobilized strepavidin-agarose||Immobilized anti-TAMRA mAb-agarose|
|89804 (10 rxn)||89805 (10 rxn)||89899 (15 rxn)||88310 (16 rxn)||88318 (6.8 µg)|
For downstream mass spectrometry analysis, peptide enrichment and/or fractionation is often necessary to detect peptides from low-abundance proteins and/or for reducing sample complexity for proteomic analysis.
Faster and higher phosphoprotein yields with the Pierce® Phosphoprotein Enrichment Kit.
|Kit||Phosphoprotein yield (ug)*||Enrichment time (hours)|
|Pierce® Phosphoprotein Enrichment Kit||300||1.5|
|Supplier Q kit||88||4.5|
|Supplier I kit||52**||3.5|
|Supplier C kit||160||3|
|Supplier E kit||Too dilute to determine||5|
**Based on maximum 1 mg load per manufacturer’s protocol.
For Research Use Only. Not for use in diagnostic procedures.