Protein Enrichment Strategies

Numerous techniques are available for the isolation of cellular proteins based on their post-translational modification (PTM) or active binding site. Protein enrichment is essential for isolating low-abundant proteins or isolating protein classes. Enrichment of specific proteins or protein complexes can most easily be accomplished using target-specific immobilized antibody or PTM specific affinity binding. The novel Thermo Scientific™ ActivX™ probes and enrichment kits enable the specific targeting of several enzyme classes, including kinases, GTPases, and serine hydrolases.

  • Comprehensive—reagents and kits for multiple protein enrichment strategies
  • Optimized—all reagents and kits are designed to maximize protein yield and reduce nonspecific binding
  • Convenient—easy-to-use spin formats enable more rapid enrichment of proteins
  • Flexible—kits can be used with either cultured mammalian cells or tissues

Choose the right protein enrichment kit for your application

protein Enrichment Kit
Glycoprotein Enrichment Kit, ConA Glycoprotein Enrichment Kit, WGA Ubiquitin Enrichment Kit Kinase Enrichment Kit with ATP Probe ActivX™ TAMRA-FP Serine Hydrolase Probe
Target Phosphorylated proteins α-linked mannose and terminal glucose residues N-acetyl glucosamine (GlcNAc) Polyubiquitin-ated proteins ATP binding sites Active serine hydrolase enzymes
labeling mechanism
Metal chelate affinity to phosphate groups Lectin affinity to sugars Lectin affinity to sugars Antibody affinity to target Biotinylated ATP analog  to active-site lysine TAMRA-labeled fluoro
phosphanate binds to active site serine
Processing time 2 hr <1 hr <1 hr <45 min 6.5 hr 3 hr
Enrichment format IMAC-agarose Immobilized conA-lectin-agarose Immobilized WGA-lectin-agarose Immobilized polyubiquitin affinity-agarose Immobilized strepavidin-agarose Immobilized anti-TAMRA mAb-agarose
(10 columns)
89804 (10 rxn) 89805 (10 rxn) 89899 (15 rxn) 88310 (16 rxn) 88318 (6.8 µg)

For downstream mass spectrometry analysis, peptide enrichment and/or fractionation is often necessary to detect peptides from low-abundance proteins and/or for reducing sample complexity for proteomic analysis.

Featured product data

Faster and higher phosphoprotein yields with the Pierce Phosphoprotein Enrichment Kit.

Kit Phosphoprotein yield (ug)* Enrichment time (hours)
Pierce Phosphoprotein Enrichment Kit 300 1.5
Supplier Q kit 88 4.5
Supplier I kit 52** 3.5
Supplier C kit 160 3
Supplier E kit Too dilute to determine 5

*From 2 mg total protein.
**Based on maximum 1 mg load per manufacturer’s protocol.