Protein Enrichment Strategies

Numerous techniques are available for the isolation of cellular proteins based on their post-translational modification (PTM) or active binding site. Protein enrichment is essential for isolating low-abundant proteins or isolating protein classes. Enrichment of specific proteins or protein complexes can most easily be accomplished using target-specific immobilized antibody or PTM specific affinity binding. The novel Thermo Scientific™ ActivX™ probes and enrichment kits enable the specific targeting of several enzyme classes, including kinases, GTPases, and serine hydrolases.

  • Comprehensive—reagents and kits for multiple protein enrichment strategies
  • Optimized—all reagents and kits are designed to maximize protein yield and reduce nonspecific binding
  • Convenient—easy-to-use spin formats enable more rapid enrichment of proteins
  • Flexible—kits can be used with either cultured mammalian cells or tissues

Choose the right protein enrichment kit for your application

 
 Phospho-
protein Enrichment Kit
Glycoprotein Enrichment Kit, ConAGlycoprotein Enrichment Kit, WGAUbiquitin Enrichment KitKinase Enrichment Kit with ATP ProbeActivX™ TAMRA-FP Serine Hydrolase Probe
TargetPhosphorylated proteinsα-linked mannose and terminal glucose residuesN-acetyl glucosamine (GlcNAc)Polyubiquitin-ated proteinsATP binding sitesActive serine hydrolase enzymes
Binding/
labeling mechanism
Metal chelate affinity to phosphate groupsLectin affinity to sugarsLectin affinity to sugarsAntibody affinity to targetBiotinylated ATP analog  to active-site lysineTAMRA-labeled fluoro
phosphanate binds to active site serine
Processing time2 hr<1 hr<1 hr<45 min6.5 hr3 hr
Enrichment formatIMAC-agaroseImmobilized conA-lectin-agaroseImmobilized WGA-lectin-agaroseImmobilized polyubiquitin affinity-agaroseImmobilized strepavidin-agaroseImmobilized anti-TAMRA mAb-agarose
 90003
(10 columns)
89804 (10 rxn)89805 (10 rxn)89899 (15 rxn)88310 (16 rxn)88318 (6.8 µg)

For downstream mass spectrometry analysis, peptide enrichment and/or fractionation is often necessary to detect peptides from low-abundance proteins and/or for reducing sample complexity for proteomic analysis.


Featured product data

Faster and higher phosphoprotein yields with the Pierce Phosphoprotein Enrichment Kit.

KitPhosphoprotein yield (ug)*Enrichment time (hours)
Pierce Phosphoprotein Enrichment Kit3001.5
Supplier Q kit884.5
Supplier I kit52**3.5
Supplier C kit1603
Supplier E kitToo dilute to determine5

*From 2 mg total protein.
**Based on maximum 1 mg load per manufacturer’s protocol.


For Research Use Only. Not for use in diagnostic procedures.