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Did you know that Applied Biosystems qPCR assays and reagents work great on Bio-Rad instruments?

It’s true. We tested Applied Biosystems qPCR assays and reagents on the Bio-Rad CFX96™ and CFX384™ real-time PCR systems**, and they performed very well.

Specifically, we conducted studies of TaqMan Gene Expression Assays with TaqMan Fast Advanced Master Mix and PowerUp SYBR Green Master Mix on the Bio-Rad CFX96 and CFX384 Real-Time PCR Detection systems.

TaqMan MGB probe-based assays don’t require optimization and are guaranteed* to perform. TaqMan Array Plate equivalents are available for the most popular Bio-Rad PrimePCR™ arrays.  We have an extremely large inventory including lncRNAs. PowerUp SYBR Green provides exceptional specificity with its proprietary dual-lock hot start mechanism.

For the TaqMan Gene Expression assays with TaqMan Fast Advanced Master Mix we looked at:

  • Singleplex vs. duplex performance
  • Reproducibility
  • Dynamic range

For PowerUp SYBR Green Master Mix we examined:

  • Reproducibility
  • Specificity
  • Dynamic range

Some highlights of the results are shown below.

TaqMan Gene Expression assays with TaqMan Fast Advanced Master Mix

From dynamic range data with 3 replicates GAPDH, GPR6, PPIA, YWHAZ.

PowerUp SYBR Green Master Mix

Benefits of TaqMan Assays:
  • Comprehensive—we’ve got you covered with over 2 million predesigned gene expression assays in nearly 30 different species. We also have a collection of >500 quality-guaranteed* fixed or flexible panels targeting specific pathways, biomarkers, or disease states.
  • Relevant content—TaqMan assays and arrays detect similar genes found on Bio-Rad™ panels, which use SYBR Green chemistry
  • Unrivaled performance—TaqMan Assays are MGB probe-based and designed using an industry-leading, verified bioinformatics pipeline, to help ensure that each assay is optimally designed and performs consistently
  • Affordable—ready-to-go format: TaqMan Gene Expression assays require no optimization and minimize the labor, expense, and bioinformatics expertise required to design qPCR assays.

If performance of a predesigned TaqMan Assay does not meet these specifications, we’ll replace it at no cost or credit your account. For more information, and to see the full terms and conditions, go to thermofisher.com/taqmanguarantee

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TaqMan Fast Advanced Master Mix offers:

  • Best-in-class performance—superior sensitivity, accuracy, dynamic range, and specificity compared to standard mixes in standard mode
  • Enhanced benchtop stability—stable at room temperature for up to 72 hr in preassembled reactions
  • Optimization for multiplexing—verified for duplexing with exogenous and endogenous internal positive control assays
  • Reduced run times on fast and standard instrumentation—optimized for fast mode on fast instruments and fast cycling conditions on standard instruments
  • Seamless transitioning to your experiments—verified with TaqMan Assays for gene expression and microRNAs, and TaqMan Array microfluidic cards

PowerUp SYBR Green Master Mix offers:

  • Exceptional specificity with dual hot-start mechanism
  • Tight reproducibility in Ct over a broad dynamic range
  • Compatibility with standard or fast cycling for results in <50 min
  • Formulation with UNG/dUTP to help prevent contamination of carryover PCR products
  • Room temperature stability for 72 hr after plates are prepared for qPCR
  • Broad instrument compatibility

Try a free sample

Interested in other qPCR applications?  Visit our Real-Time PCR Reagents and Kits section where you can request a free sample or learn more about products for gene expression, genotyping, copy number variation analysis, virus detection, and multiplexing, one-step qPCR master mixes, and general purpose reagents.

 

For more information about the TaqMan guarantee, and to see the full terms and conditions, go to thermofisher.com/taqmanguarantee

**Bio-Rad, CFX96 and CFX384 are trademarks of Bio-Rad Laboratories, Inc., which is not affiliated with Thermo Fisher. The statements herein have not been reviewed or endorsed by Bio-Rad.