TaqMan Real-Time PCR Assays

Gene expression analysis

• Assays help detect the highest number of transcript variants possible
• 2.8 million assays, >32 species
• Predesigned gene expression assays
• Custom gene expression assays

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MicroRNA analysis

• Detect targets with as few as 60 copies of input microRNA
• Compatible with tissue & biofluids including serum & plasma
• 30K assays
• Predesigned microRNA assays
• Custom microRNA assays

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Genetic variation analysis

• Predesigned assays for SNP genotyping & drug metabolism (DME); 17 million assays
• 1.8 million copy number variation (CNV) assays
• Predesigned SNP genotyping assays
• Custom SNP genotyping assays

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Mutation detection analysis

• Enhance your specificity, sensitivity, and workflow efficiency with TaqMan mutation detection assays; 900 assays
• Predesigned mutation detection assays

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qPCR Assay Design Hub

Discover tools and services for designing real-time PCR (qPCR) custom assays, including applications like gene expression, SNP genotyping, and miRNA. Access tools for primer and probe design or request custom assay designs from our bioinformatics experts.

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Custom qPCR assays

Use our TaqMan Custom Design Assay Tool to easily design your own assay based on your target sequence of interest or submit the primer/probe sequences of your own design. Each custom assay is a mix of forward primer, reverse primer, and FAM and/or VIC dye-labeled TaqMan MGB probe.

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TaqMan probes and qPCR primers

Design your own TaqMan probes and qPCR primers from your choice of dye-labeled TaqMan MGB, TAMRA, or QSY custom probes and Applied Biosystems unlabeled, sequence detection primers.

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Endogenous control assays for qPCR

Predesigned TaqMan probe and primer sets enable you to normalize the amount of sample RNA or DNA in a reaction. Controls are available for the most common human, mouse, rat, and eukaryotic genes.

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TaqMan assays & arrays for oncology biomarkers

Explore the extensive range of TaqMan assays and arrays, designed to advance your oncology research. Ideal for gene expression analysis, oncogene detection, biomarker identification, mutation analysis, and clinical research.

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Duplexing with TaqMan assays

Duplexing in qPCR is the simultaneous detection of two target sequences in a single reaction. Its key benefits come from minimizing the impact of pipetting errors and maximizing experimental efficiency.

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Multiplexing with TaqMan probes

Enable multiplexing of up to six targets in one qPCR reaction with TaqMan probes.

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Non-coding RNA assays for qPCR

TaqMan non-coding RNA assays offer a highly reliable way to measure expression of long non-coding transcripts. These assays help provide the high specificity and sensitivity you need for quantification and validation studies.

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Specialty manufacturing

If your research application requires special assay manufacturing modifications, such as a different dye or assay volume, or you would like to have your assays plated in a specific way, our custom services can help.

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Digital PCR assays and kits

TaqMan assays are now available for digital PCR (dPCR) with the QuantStudio Absolute Q System, including the Universal DNA dPCR Master Mix and AutoRun dPCR Suite. Explore assays for a wide range of applications, all backed by our performance guarantee.

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TaqMan assay plates and cards

TaqMan arrays for high-throughput gene expression analysis. Available in plates, microfluidic cards, and OpenArray formats.

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QuantStudio real-time PCR systems

Explore new scientific frontiers with our advanced QuantStudio real-time PCR systems, designed to empower your research with advanced technology and continuous innovation tailored to your strategies and budget.

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Real-time PCR reagents and kits

Choose Applied Biosystems real-time PCR master mixes for optimized qPCR analysis enabling accurate nucleic acid quantification with reliable results, backed by over 25 years of innovation from Thermo Fisher Scientific.

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