Since there is no single parameter that defines programmed cell death, a combination of techniques is recommended for the reliable detection of apoptosis. Our multiparametric apoptosis assay kits are designed to selectively differentiate apoptotic cells from living and necrotic cells in a single cell population. The kits are designed to detect various apoptotic parameters in a single flow cytometry assay, helping to dissect the early and middle stages of the apoptotic response.

Multiparametric apoptosis analysis using the Alexa Fluor 488 Annexin V/Dead Cell Apoptosis kit with PI. Jurkat cells (T-cell leukemia, human) were treated with 10 μM camptothecin for four hours (bottom panel) or left untreated as a control (top panel). Cells were then treated with the reagents in the kit and analyzed by flow cytometry. Note that the camptothecin-treated cells (bottom panel) have a higher percentage of apoptotic cells (indicated by “A”) than the basal level of apoptosis seen in the control cells (top panel). L = live cells, D = dead cells.
 Product Parameter 1 Parameter 2 Parameter 3 Excitation (nm) Cat. No.
Single Channel Annexin V/Dead Cell Apoptosis Kit, with Alexa Fluor 488 annexin V and SYTOX Green Membrane Asymmetry Necrosis 488 V13240
Alexa Fluor 488 Annexin V/Dead Cell Apoptosis Kit, with PI Membrane Asymmetry Necrosis 488 V13241
FITC Annexin V/Dead Cell Apoptosis Kit, with PI Membrane Asymmetry Necrosis 488 V13242
Membrane Permeability/Dead Cell Apoptosis Kit, with YO-PRO-1 and PI Membrane Asymmetry Necrosis 488 V13243
Chromatin Condensation Apoptosis Kit, with Hoechst 33342 and PI Chromatin Condensation Necrosis UV/488 V13244
Chromatin Condensation/Membrane Permeability/Dead Cell Apoptosis Kit, with Hoechst 33342, YO-PRO-1, and PI Chromatin Condensation Necrosis UV/488 V23201
PE Annexin V/Dead Cell Apoptosis Kit, with SYTOX Green Membrane Asymmetry Necrosis 488 V35112
APC Annexin V/Dead Cell Apoptosis Kit, with SYTOX Green Membrane Asymmetry Necrosis 488/633 V35113
Metabolic Activity/Annexin V/Dead Cell Apoptosis Kit, with C12 resazurin, APC annexin V, and SYTOX Green Mitochondrial Health Membrane Asymmetry Necrosis 488/633 V35114
Mitchondrial Membrane Potential/Annexin V Apoptosis Kit, with MitoTracker Red and Alexa Fluor 488 annexin V Mitochondrial Health Membrane Asymmetry 488 V35116
Violet Annexin V/Dead Cell Apoptosis Kit, with Pacific Blue annexin V/SYTOX AADvanced Membrane Asymmetry Necrosis 405/488 A35136
Violet Membrane Permeability/Dead Cell 1 Apoptosis Kit, with PO-PRO-1 and 7-aminoactinomycin D Membrane Asymmetry Necrosis 405/488 V35123
Violet Chromatin Condensation/Dead Cell Apoptosis Kit, with Vybrant DyeCycle Violet and SYTOX AADvanced Chromatin Condensation Necrosis 405/488 A35135

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