Flow cytometry analysis of a sample with dead, apoptotic, and live cells

There are many different apoptosis assays for flow cytometry because cell death cascades are complex and dynamic, underscoring the importance of a multi-parametric approach to the assessment of apoptosis. Since no single parameter defines programmed cell death, a combination of techniques is recommended for flow cytometry apoptosis detection.

Flow cytometry apoptosis detection assays

We offer a wide variety of apoptosis assays for flow cytometry that detect changes in the plasma and mitochondrial membranes, caspase activity, nuclear condensation, and fragmentation as possible indicators of apoptosis. Mix and match our standalone reagents or choose from our selection of multi-parametric apoptosis assay kits that are designed to selectively differentiate apoptotic cells from living and necrotic cells in a single cell population.

Selection Guide: search apoptosis assays for flow cytometry

Select flow cytometry apoptosis detection reagents based on the laser excitation source and common emission filters.

UV laser (~350 nm) reagents

Emission filter 379/28 nm 440/40 nm
Annexin V conjugates  
Membrane permeability & chromatin condensation  

Violet laser (405 nm) reagents

Yellow laser (561 nm) reagents

Emission filter 585/16 nm 620/15 nm
Annexin V conjugates  
Dynamic changes in mitochondrial membrane potential  
End point assays for mitochondrial membrane potential  

Red laser (637 nm) reagents

UV laser (~350 nm) reagents

Emission filter 379/28 nm 440/40 nm
Annexin V conjugates  
Membrane permeability & chromatin condensation  

Violet laser (405 nm) reagents

Yellow laser (561 nm) reagents

Emission filter 585/16 nm 620/15 nm
Annexin V conjugates  
Dynamic changes in mitochondrial membrane potential  
End point assays for mitochondrial membrane potential  

Red laser (637 nm) reagents


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