Applied Biosystems SYBR Green master mixes are designed for quantitative real-time PCR using a set of two PCR primers that flank the target region. The master mixes greatly simplify your experiment setup as they contain everything you need: buffer, dNTPs, thermostable hot-start DNA polymerase, and, of course, SYBR Green dye. Just add your sample and PCR primer pair and you're ready to run your qPCR.
Which SYBR Green master mix is right for you?
SYBR Green master mix formulations have improved over the years, as researchers have demanded improved sensitivity, greater specificity, enhanced reproducibility, faster time-to-results, and protection from contamination. Although we offer many choices of SYBR Green master mixes, following are the recommended and highest-performing master mixes.
| Recommended master mix | PowerTrack SYBR Green Master Mix | PowerUp SYBR Green Master Mix |
|---|---|---|
| Applications | Gene expression analysis NGS library quantification Microbe detection ChIP DNA analysis | Gene expression analysis NGS library quantification Microbe detection ChIP DNA analysis |
| Recommended primer Tm | 55–65°C | 60°C |
| Sample tracking dye (pipetting QC) | Yes | No |
| Specificity | High | High |
| Sensitivity | High | High |
| Recommended primer concentration | 300–800 nM | 300–800 nM |
| Preassembled reaction benchtop stability | Up to 8 hours | Up to 72 hours |
| Hot start | Antibody-mediated | Dual-lock hot start mechanism |
| Cycling mode | Standard (~60 min) Fast (~30 min) | Standard (~60 min) Fast (~30 min) |
| Carryover contamination control | Includes heat-labile UNG and a blend of dTTP/dUTP | Includes heat-labile UNG and a blend of dTTP/dUTP |
| Instrument compatibility | Compatible with all Applied Biosystems real-time PCR instruments, Bio-Rad iQ™5 and CFX™ (96 and 384), and Roche LightCycler™ 480 systems | Compatible with all Applied Biosystems real-time PCR instruments, Bio-Rad iQ™5 and CFX™ (96 and 384), Roche LightCycler™ 480, and Stratagene Mx3005P™ systems |
Looking for more options?
See the entire range of SYBR Green master mixes available from Thermo Fisher Scientific below.
 |  |  |  |  |  |  |  | Comments | |
| Applied Biosystems products | Wide instrument compatibility, and the best choice for Applied Biosystems instruments. All mixes include ROX dye. | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| PowerTrack SYBR Green Master Mix | • | • | • | •• | • | • | Our newest and best mix. Designed to increase your efficiency and reduce pipetting errors via sample tracking dye. Formulated for excellent specificity, sensitivity, fast or standard cycling, and wide instrument compatibility. | ||
| PowerUp SYBR Green Master Mix | • | • | • | • | •• | • | • | Formulated for tight, hot-start control of Taq polymerase, high specificity and sensitivity, fast or standard cycling, and wide instrument compatibility | |
| Power SYBR Green Master Mix | • | • | A solid SYBR Green master mix without UNG | ||||||
| SYBR Select Master Mix | • | • | • | •• | • | Our brightest mix. May be too bright for some older instruments. | |||
| Fast SYBR Green Master Mix | • | • | • | • | The original fast cycling SYBR Green master mix | ||||
| SYBR Green Master Mix | • | • | The original SYBR Green master mix | ||||||
| MeltDoctor HRM Master Mix | • | Formulated specifically for high resolution melt (HRM) analysis | |||||||
| Thermo Scientific products | Mixes optimized for specific instruments from Thermo Fisher Scientific and other suppliers. Versions with separate ROX, low ROX, and high ROX available. | ||||||||
| Luminaris Color HiGreen qPCR Master Mix | • | • | • | V | • | Recommended alternative products: PowerTrack and PowerUp SYBR Green master mixes | |||
| Maxima SYBR Green qPCR Master Mix | • | V | Recommended alternative products: PowerTrack and PowerUp SYBR Green master mixes | ||||||
| DyNAmo ColorFlash SYBR Green qPCR Kit | • | • | • | V | • | Recommended alternative products: PowerTrack and PowerUp SYBR Green master mixes | |||
| Absolute QPCR Mix, SYBR Green | V | Our only SYBR Green mix without dUTP/UNG. Recommended alternative products: PowerTrack and PowerUp SYBR Green master mixes. | |||||||
| Invitrogen products | Mixes optimized for specific instruments from Thermo Fisher Scientific and other suppliers. Versions with separate ROX, low ROX and high ROX available. | ||||||||
| SYBR GreenER qPCR SuperMix | • | • | V | Recommended alternative products: PowerTrack and PowerUp SYBR Green master mixes | |||||
| Express SYBR GreenER qPCR SuperMix | • | • | •• | V | Recommended alternative products: PowerTrack and PowerUp SYBR Green master mixes | ||||
| Platinum SYBR Green qPCR SuperMix-UDG | • | • | V | Recommended alternative products: PowerTrack and PowerUp SYBR Green master mixes | |||||
1. • = master mix includes uracil N-glycosylase (UNG); •• = master mix includes a heat-labile form of UNG, which improves master mix performance compared to the standard UNG.
2. Master mix includes ROX, a passive reference dye; V = varies, different master mix versions are available with different amounts of ROX dye, or ROX included in a separate tube.
For Research Use Only. Not for use in diagnostic procedures.
