packaging with vials for 2-mercaptoethylamine-HCl

Protein denaturing and reducing agents are available as purified powders, convenient solutions, and solid phase reagents.

Denaturing and reducing agent selection guide

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What are protein reducing agents?

Reducing agents can be used to disrupt, or reduce, disulfide bonds in peptides and proteins. Disulfide reducing agents include tris (2-carboxyethyl) phosphine hydrochloride (TCEP), beta-mercaptoethanol (BME), and dithiothreitol (DTT). Usage of these agents is important for analyzing individual proteins.

What are protein denaturing agents?

Denaturing agents can be used to unfold and alter protein structure. Additionally, they disrupt water interactions and promote hydrophobic protein and peptide solubilization, elution, and refolding. These agents include urea and guanidine hydrochloride.

Selection guide for denaturing and reducing agents

ProductFunctionThiol-freeFormCat. No.
Bond-Breaker TCEP Solution, Neutral pHStable 0.5 M solution of TCEP reducing agent for protein disulfide bonds, odor-free and suitable as a 10X stock to make reducing SDS-PAGE sample buffers.YesLiquid77720
TCEP-HClPure crystalline Tris (2-carboxyethyl) phosphine hydrochloride, (TCEP, CAS 5961-85-3) is a thiol-free reductant for protein and peptide disulfide bonds.YesSolid20490
TCEP-HCl, No-Weigh FormatPre-aliquoted format of protein disulfide reducing agent prevents the loss of reagent reactivity and contamination over timeYesA35349
TCEP-HCl, Premium Grade
Highly characterized version of a popular protein disulfide reducing agent thoroughly tested and fully documented.YesPG82090
Immobilized TCEP Disulfide Reducing Gel
Immobilized TCEP disulfide reducing gel is tris(2-carboxyethyl)phosphine covalently immobilized to agarose resin to help enable the efficient reduction of peptide and protein disulfide bonds without contaminating samples with reducing agentYesLiquid77712
Immobilized Reductant Columns
Immobilized reductant column contains resin to which a thiol-based reducing agent has been immobilized to enable the solid-phase reduction of peptide and protein disulfide bondsNoLiquid77701
2-Mercaptoethanol
Pure liquid (14 M), beta-mercaptoethanol (BME, 2BME, 2-ME, b-mer, CAS 60-24-2) is a thiol reducing agent for cleaving protein disulfide bonds (cystine).
NoLiquid35602BID
2-Mercaptoethylamine-HCl
Pure crystalline 2-aminoethanethiol (2-MEA-HCl, also called cysteamine-HCl, CAS 156-57-0), selectively reduces antibody hinge-region disulfide bonds
NoSolid20408
DithiothreitolConvenient 48-microtube tray containing dry, stable, single-use aliquots of DTTNoPowder20290
DTT (Dithiothreitol), No-Weigh Format
Convenient 48-microtube tray containing dry, stable, single-use aliquots of DTTNoDry aliquotsA39255
Hydroxylamine-HClHydroxylamine-HCl (NbOH.HCl) (CAS 7803-49-8) is used to cleave Asn-Gly peptide bonds or to deprotect sulfhydryls following S-acetylthioacetate modification with SATA reagentSolid26103
Disulfide bond reduction using TCEP
Reduction of organic disulfide bonds using TCEP.
ProductFunctionFormCat. No.
8M Guanidine-HCl SolutionAccurately formulated, highly pure, ready-to-use solution for the solubilization of hydrophobic proteins and peptidesLiquid24115
Guanidine-HClHighly pure solid that is easily dissolved and pH-adjusted for the solubilization of hydrophobic proteins and peptidesPowder24110
UreaPure, crystalline protein denaturant that increases solubility of hydrophobic molecules and acts as a general protein denaturant via unfolding and altering their 3D structuresPowder29700
ProductFunctionThiol-freeFormCat. No.
Bond-Breaker TCEP Solution, Neutral pHStable 0.5 M solution of TCEP reducing agent for protein disulfide bonds, odor-free and suitable as a 10X stock to make reducing SDS-PAGE sample buffers.YesLiquid77720
TCEP-HClPure crystalline Tris (2-carboxyethyl) phosphine hydrochloride, (TCEP, CAS 5961-85-3) is a thiol-free reductant for protein and peptide disulfide bonds.YesSolid20490
TCEP-HCl, No-Weigh FormatPre-aliquoted format of protein disulfide reducing agent prevents the loss of reagent reactivity and contamination over timeYesA35349
TCEP-HCl, Premium Grade
Highly characterized version of a popular protein disulfide reducing agent thoroughly tested and fully documented.YesPG82090
Immobilized TCEP Disulfide Reducing Gel
Immobilized TCEP disulfide reducing gel is tris(2-carboxyethyl)phosphine covalently immobilized to agarose resin to help enable the efficient reduction of peptide and protein disulfide bonds without contaminating samples with reducing agentYesLiquid77712
Immobilized Reductant Columns
Immobilized reductant column contains resin to which a thiol-based reducing agent has been immobilized to enable the solid-phase reduction of peptide and protein disulfide bondsNoLiquid77701
2-Mercaptoethanol
Pure liquid (14 M), beta-mercaptoethanol (BME, 2BME, 2-ME, b-mer, CAS 60-24-2) is a thiol reducing agent for cleaving protein disulfide bonds (cystine).
NoLiquid35602BID
2-Mercaptoethylamine-HCl
Pure crystalline 2-aminoethanethiol (2-MEA-HCl, also called cysteamine-HCl, CAS 156-57-0), selectively reduces antibody hinge-region disulfide bonds
NoSolid20408
DithiothreitolConvenient 48-microtube tray containing dry, stable, single-use aliquots of DTTNoPowder20290
DTT (Dithiothreitol), No-Weigh Format
Convenient 48-microtube tray containing dry, stable, single-use aliquots of DTTNoDry aliquotsA39255
Hydroxylamine-HClHydroxylamine-HCl (NbOH.HCl) (CAS 7803-49-8) is used to cleave Asn-Gly peptide bonds or to deprotect sulfhydryls following S-acetylthioacetate modification with SATA reagentSolid26103
Disulfide bond reduction using TCEP
Reduction of organic disulfide bonds using TCEP.
ProductFunctionFormCat. No.
8M Guanidine-HCl SolutionAccurately formulated, highly pure, ready-to-use solution for the solubilization of hydrophobic proteins and peptidesLiquid24115
Guanidine-HClHighly pure solid that is easily dissolved and pH-adjusted for the solubilization of hydrophobic proteins and peptidesPowder24110
UreaPure, crystalline protein denaturant that increases solubility of hydrophobic molecules and acts as a general protein denaturant via unfolding and altering their 3D structuresPowder29700

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For Research Use Only. Not for use in diagnostic procedures.